Response of wastewater-based epidemiology predictor for the second wave of COVID-19 in Ahmedabad, India: A long-term data Perspective.

In this work, we present an eight-month longitudinal study of wastewater-based epidemiology (WBE) in Ahmedabad, India, where wastewater surveillance was introduced in September 2020 after the successful containment of the first wave of COVID-19 to predict the resurge of the infection during the second wave of the pandemic. The study aims to elucidate the weekly resolution of the SARS-CoV-2 RNA data for eight months in wastewater samples to predict the COVID-19 situation and identify hotspots in Ahmedabad. A total of 287 samples were analyzed for SARS-CoV-2 RNA using RT-PCR, and Spearman's rank correlation was applied to depict the early warning potential of WBE. During September 2020 to April 2021, the increasing number of positive wastewater influent samples correlated with the growing number of confirmed clinical cases. It also showed clear evidence of early detection of the second wave of COVID-19 in Ahmedabad (March 2021). 258 out of a total 287 samples were detected positive with at least two out of three SARS-CoV-2 genes (N, ORF- 1 ab, and S). Monthly variation represented a significant decline in all three gene copies in October compared to September 2020, followed by an abrupt increase in November 2020. A similar increment in the gene copies was observed in March and April 2021, which would be an indicator of the second wave of COVID-19. A lead time of 1-2 weeks was observed in the change of gene concentrations compared with clinically confirmed cases. Measured wastewater ORF- 1 ab gene copies ranged from 6.1 x 102 (October 2020) to 1.4 x 104 (November 2020) copies/mL, and wastewater gene levels typically lead to confirmed cases by one to two weeks. The study highlights the value of WBE as a monitoring tool to predict waves within a pandemic, identify local disease hotspots within a city, and guide rapid management interventions.

Resolution of conflict of reduced sludge production with EBPR by coupling OSA to A2/O process in a pilot scale SBR.

The pinnacle of all the efforts of nutrient removal is practically put-down the moment biological cells are lysed, hydrolyzed or digested causing subsequent reappearance of assimilated nitrogen and phosphorus in any biological process. While sludge reduction requires high SRT, the enhanced phosphorus assimilative uptake demands low SRT. A novel reactor configuration for enhanced sludge and phosphorus removal was put to test by incorporating a side stream anaerobic reactor to an Anaerobic-Anoxic-Aerobic (A2O) SBR with a pre-anoxic chamber and an influent receiving inlet anaerobic reactor. The reactor was operated at the average and lowest range of prevailing carbon/phosphorus (C/P) ratio of 50 and 15 in the sewage. The phosphorus enrichment was 0.0469-0.135 mgTP/mgVSS resulting in 1.76-5.05-fold increase from cellular content by virtue of maintaining sludge recycle from SBR aeration tank to side stream anaerobic reactor from 3.78 to 9.78 (average 4.4-8.2) gVSS/gVSS present in the reactor. However, the sludge was also reduced from 3% to 51% on an average basis during the same recirculation regime. This novel configuration consists of an inlet anaerobic reactor, one pre-anoxic chamber and one intermittent oxic anoxic reaction SBR and a side stream anaerobic reactor. The first anaerobic reactor at inlet followed by pre-anoxic chamber was provided for increased ortho-p released and nitrification respectively and a side stream anaerobic reactor for sludge reduction through sludge fasting mechanism. The EBPR and lesser sludge growth were two conflicting parameters reconciled to the extent that if sludge recycled up to 6.41 gVSS/gVSS the sludge growth would be reduced by 25% and phosphorus enrichment could be attained up to 3.46 times the stoichiometric value. Any further recirculation would reduce the sludge further but at the expense of enhanced phosphorus uptake as released phosphorus from side stream anaerobic reactor also recycled back to main SBR causing looping and at more than 6.41gVSSrecycled/gVSS it nullified the enhanced effect.

Evaluation of water quality using water quality index, synthetic pollution index, and GIS technique: a case study of the river Gomti, Lucknow, India.

The river Gomti, one of India's most polluted rivers, passing through Lucknow, Uttar Pradesh, India, has been selected for this study. An attempt has been made to assess its water quality status by combining the water quality index (WQI) and synthetic pollution index (SPI). Further, the data integration with the geographic information system (GIS) along with twelve water quality parameters for the seven sampling stations (S1 to S7) over 5 years (2013-2017) has been performed. The study area showed a variation of WQI from 78.993 to 249.388 and SPI from 0.868 to 2.096 in 5 years. The map interpolated through GIS revealed that the WQI falls into the category of severely polluted (76-100) and unsuitable for human consumption (> 100), while SPI lies in the category of moderately polluted (0.5-1.0) and severally polluted (1.0-3.0). The BOD and COD were found to significantly influence the WQI and SPI scores. With the constant release of waste effluents into the river, all selected parameters increased from S1 to S7. Based on the study, effective wastewater management is immediately required to improve water quality and support any sustainable river restoration plan.

Optimization of Fenton process for concurrent COD removal and lower sludge production: Process intensification and impact of reagents dosing mode.

The versatile applications of the advanced oxidation processes (AOPs) are considered promising options for wastewaters treatment. Among AOPs, the classical Fenton process (CFP) is well recognized in literature due to its notable advantages. But the drawbacks associated with the CFP are the main barrier to its extensive applications. The primary process's drawbacks are the high sludge production and high reagents requirement, making the process unsustainable. Hence, the present study aims to overcome these drawbacks and improve the process's performance. The reagents (Fe2+ and H2O2) dosage were adopted as the variable parameters and varied throughout the experiments at fixed pH 3 and a reaction time of 45 min. The experiments were performed in three different stages; stage I (S-I: single-step reagents dosing mode), stage II (S-II: two-step reagents dosing mode), and stage III (S-III: three-step reagents dosing mode) and landfill leachate was taken as a sample. The dosing mode of reagents was found to be a crucial influencing factor to improve the overall Fenton process's performance by effectively utilizing the hydroxyl radicals (•OH) and avoiding the scavenging reactions. The two-step reagents dosing mode was found better, i.e., 52 ± 2% chemical oxygen demand (COD) removal and 40 ± 2 mL sludge production compared to S-I and S-III with approximately 10-15% extra COD removal and low sludge production at fewer reagents dosage (Fe2+ dosage 798 mg/L and H2O2 dosage 2098 mg/L). The lesser sludge production reduces secondary environmental pollution, and low reagents dosage makes the process cost-effective. The optimization conditions were obtained by response surface methodology (RSM), and high coefficient of determination (R2 > 0.95) values confirm the adequacy of the models obtained. Furthermore, few experiments were performed on the sludge reusability, and valuable remarks were highlighted. Overall, the purpose of this study is to enhance the CFP's performance by overcoming its drawbacks.

A chronicle of SARS-CoV-2: Seasonality, environmental fate, transport, inactivation, and antiviral drug resistance.

In this review, we present the environmental perspectives of the viruses and antiviral drugs related to SARS-CoV-2. The present review paper discusses occurrence, fate, transport, susceptibility, and inactivation mechanisms of viruses in the environment as well as environmental occurrence and fate of antiviral drugs, and prospects (prevalence and occurrence) of antiviral drug resistance (both antiviral drug resistant viruses and antiviral resistance in the human). During winter, the number of viral disease cases and environmental occurrence of antiviral drug surge due to various biotic and abiotic factors such as transmission pathways, human behaviour, susceptibility, and immunity as well as cold climatic conditions. Adsorption and persistence critically determine the fate and transport of viruses in the environment. Inactivation and disinfection of virus include UV, alcohol, and other chemical-base methods but the susceptibility of virus against these methods varies. Wastewater treatment plants (WWTPs) are major reserviors of antiviral drugs and their metabolites and transformation products. Ecotoxicity of antiviral drug residues against aquatic organisms have been reported, however more threatening is the development of antiviral resistance, both in humans and in wild animal reservoirs. In particular, emergence of antiviral drug-resistant viruses via exposure of wild animals to high loads of antiviral residues during the current pandemic needs further evaluation.

Co-occurring indicator pathogens for SARS-CoV-2: A review with emphasis on exposure rates and treatment technologies.

Scientific advancements from 2002 to 2020 for coronaviruses, i.e., SARS-CoV and MERS-CoV outbreaks, could lead towards a better understanding of the exposure to a health crisis. However, data on its transmission routes and persistence in the environment is still in need of the hour. In this review, we discuss the impact of environmental matrices on dealing with the consequences of the global COVID-19 outbreak. We have compiled the most recent data on the epidemiology and pathogenesis of the diseases. The review aims to help researchers and the larger public recognize and deal with the consequences of co-occurring viral indicators for COVID-19 and provide nano-technological perspectives of possible diagnostic and treatment tools for further studies. The review highlights environmental wastes such as hospital wastewater effluents, pathogen-laden waste, pathogen-laden ground/surface water, wastewater sludge residues and discusses their potential remediation technologies, i.e., pathogen-contaminated soil disposal, municipal and medical solid waste collection, recycling, and final disposal. Finally, holistic suggestions to tackle environmental-related issues by the scientific community have been provided, where scientists, consultants may involve in a tiered assessment from the hazard to risk management in the post-COVID-19 world.

Future liasing of the lockdown during COVID-19 pandemic: The dawn is expected at hand from the darkest hour.

The lockdown during COVID-19 pandemic has converted the world into new experimental laboratories, which may reveal temporal or spatial comparative analysis data. However, some startling information is gathered in terms of reduced premature mortality cases associated with air and water quality improvement, enhanced e-learning on a broader platform, work from home, and successful e-health. The decline in vehicular density on roads and congestion leads to reduced energy consumption and associated greenhouse gases (GHG) and other pollutants emission. The lockdown has also been identified as a possible emergency measure to combat severe air pollution episodes. Similarly, industrial pollution has been recognized as one of the primary causes of water resource pollution and would, therefore, bring change in policy vis-à-vis groundwater pollution control. Our findings suggest that the results of successful e-learning and work from home would be a permanent shift from conventional modes in the near future due to a drastic reduction in socio-economic cost. Our critical analysis also highlights that with such temporary lockdown measures acute/chronic ill-effects of anthropogenic perturbations on planet earth can be effectively estimated through sociocultural, socioeconomical and socio-political/sociotechnological nexus.

Reduced sludge growth at high bulk liquor dissolved oxygen induced by increased secondary cell maintenance.

Sludge reduction by physico-chemical methods results in the buildup of chemicals, which may require further treatment. Owing these reasons various biologically sustainable methods of sludge reduction including the application of high oxygenation have been successfully tested. Experiments on actual sewage in two lab-scale sequencing batch reactors (SBRs) were conducted under normal (1.5-2.5 mgDO/L) and high dissolved oxygen (DO) (HDO: 3-6.5 mgDO/L) regimes. It was observed that microorganism allocated substrate between maintenance and growth in the form of maintenance coefficient. Which could be induced by endogenous respiration owing to high solids retention time (SRT), predation on bacteria, chemical toxicity, adverse environment, and viral attack on bacteria. The wastewater treatment process may experience one or more maintenance inducing factors; nevertheless, high SRT and prevailing environmental conditions are imminent and thus considered as primary maintenance (mp), while remaining are classified as secondary maintenance (ms). Average yield coefficient reduction at HDO was 32.7% and 28.2% compared to stoichiometric and at normal DO, respectively. The observed primary and secondary maintenance was 0.11gCOD/gVSS.d (±0.01) at an SRT of 25.2 d (±2.0) and 0.096 g 0.1 gCOD/gVSS.d (±0.045) at an SRT of 24.2 d (±3.6d), respectively. The results obtained under the study are not as precise as on pure culture and defined substrate, nevertheless, it gives an idea that how stress factors inducing maintenance need to be addressed more seriously and objectively while managing our efforts on sludge reduction.

Modified septic tank-anaerobic filter unit as a two-stage onsite domestic wastewater treatment system.

This study demonstrates the performance evaluation of a uniquely designed two-stage system for onsite treatment of domestic wastewater. The system consisted of two upflow anaerobic bioreactors, a modified septic tank followed by an upflow anaerobic filter, accommodated within a single cylindrical unit. The system was started up without inoculation at 24 h hydraulic retention time (HRT). It achieved a steady-state condition after 120 days. The system was observed to be remarkably efficient in removing pollutants during steady-state condition with the average removal efficiency of 88.6 +/- 3.7% for chemical oxygen demand, 86.3 +/- 4.9% for biochemical oxygen demand and 91.2 +/- 9.7% for total suspended solids. The microbial analysis revealed a high reduction (>90%) capacity of the system for indicator organism and pathogens. It also showed a very good endurance against imposed hydraulic shock load. Tracer study showed that the flow pattern was close to plug flow reactor. Mean HRT was also found to be close to the designed value.

Effects of multi-metal toxicity on the performance of sewage treatment system during the festival of colors (Holi) in India.

The present study investigated the effects of heavy metals (Ni, Zn, Cd, Cu, and Pb) toxicity on the performance of 18 MLD activated sludge process-based sewage treatment plant (STP) during celebration of Holi (festival of colors in India). The composite sampling (n = 32) was carried out during the entire study period. The findings show a significant decrease in chemical oxygen demand removal efficiency (20%) of activated sludge system, after receiving the heavy metals laden wastewater. A significant reduction of 40% and 60% were observed in MLVSS/MLSS ratio and specific oxygen uptake rate, which eventually led to a substantial decrease in biomass growth yield (from 0.54 to 0.17). The toxic effect of metals ions was also observed on protozoan population. Out of the 12 mixed liquor species recorded, only two ciliates species of Vorticella and Epistylis exhibited the greater tolerance against heavy metals toxicity. Furthermore, activated sludge shows the highest metal adsorption affinity for Cu, followed by Zn, Pb, Ni, and Cd (Cu > Zn > Pb > Ni > Cd). Finally, this study proves the robustness of activated sludge system against the sudden increase in heavy metal toxicity since it recovered the earlier good quality performance within 5 days.

Retrospective of ecological approaches to excess sludge reduction.

The problem of excess sludge handling produced during wastewater treatment is undeniable reality of grave concern with increasingly stringent legislations. The sludge synthesis yield being 0.4-0.6 kgVSS/kgCOD (0.57-0.8 kgCODcell/kgCOD), results in high power consumption on its digestion and therefore taken considerable attention to achieve sustainable strategies. Solids reduction by physico-chemical methods results in buildup of chemicals. This may present risk to the environment and may require further treatment to remove the chemicals of concern in future. Wastewater sludge reduction upto 100% by biological, sustainable, non-hazardous, and environment friendly methods has been successfully tested at different levels. Therefore, above reasons were sufficient driving forces to confine this review to non-chemically assisted processes. Similarly, the thermally assisted processes result in high carbon footprint and excluded from the scope of this review. Enough has been reviewed on sludge reduction, as numbers of articles on the same subject with different angles have been reported, still the progress in the last few years is missing; hence, special emphasis is given herewith to highlight the efforts of the last five years.

Fate of coliforms and pathogenic parasite in four full-scale sewage treatment systems in India.

The occurrence and removal of fecal indicators (total coliforms (TC), fecal coliforms (FC), fecal streptococci (FS)) and pathogens (helminthes eggs) were studied in various municipal wastewater treatment processes (UASB + FPU, ASP, EA, WSP). The reductions in TC and FC concentrations were usually between 2.0 and 2.5 log units in up-flow anaerobic sludge blanket reactor incorporated with final polishing unit (UASB + FPU). Almost similar reduction was observed in activated sludge process system (ASP) and waste stabilization ponds system (WSP), while it was log 3.0 in extended aeration system (EA). UASB + FPU and WSP systems were observed more efficient to reduce helminthes eggs at almost 100%, whereas only 97% removal was observed in case of ASP and EA system. In addition to monitoring of indicator organisms, turbidity, suspended solids (SS), and biochemical oxygen demand (BOD) were used as indirect measure of the potential presence of microorganisms. Interrelationship of BOD, SS, and turbidity with fecal indicator bacteria concentration in influent and effluent manifest that improvement of the microbiological quality of wastewater is strongly linked to the removal of BOD and SS.