ABSTRACT
We studied the changes in superoxide dismutase activity in organs of Galleria mellonella larvae infected with two strains of Bacillus thuringiensis. A considerable increase in superoxide dismutase activity was observed at the initial stages of infection, later the enzyme activity decreased and this decrease was timed to cessation of feeding and development of sepsis in the infected larvae. Changes in the enzyme activity in the organs of larvae infected with a highly virulent strain of B. thuringiensis correlated with the stages of infection. Involvement of superoxide dismutase in prevention of oxidative stress in the infected larvae is discussed.
Subject(s)
Bacillus thuringiensis , Bacteremia/metabolism , Moths/metabolism , Moths/microbiology , Superoxide Dismutase/metabolism , Animals , Larva/metabolism , Larva/microbiology , Oxidative StressABSTRACT
Structural changes in hemogram of Gypsy moth larvae from a population in the phase of quantity increase are studied morphometrically and cytochemically. The counts of granulocytes and prohemocytes are increased, as are the counts of hemocytes possessing phenol oxidase activity and hemocytes reducing nitroblue tetrazolium. Atypical virus morphogenesis is observed.
Subject(s)
Blood Cell Count , Moths/virology , Nucleopolyhedroviruses/physiology , Animals , Larva/ultrastructure , Larva/virology , Microscopy, Electron , Moths/embryology , Moths/ultrastructureABSTRACT
Opisthorchis somatic, metabolic, and tegumental antigens were studied by using immunoblotting. It was shown that among several antigenic components, only the somatic protein with a molecular weight of about 100 kD failed to react with noninvased human sera.