GC/MS analysis of the hydrodistilled essential oils and volatiles from the aerial parts of Cannabis sativa L.

Cannabis sativa L. has been recognised as a narcotic drug in different parts of the world. The current report deals with the screening of the chemical profiles of the essential oils (EOs) and volatiles obtained from the aerial ports of Cannabis sativa L. (APCS) using classical hydrodistillation (HD), solid phase microextraction (SPME) along with a new technique namely innovative cigarette ignition (Incigig); all in combination with GC/MS. The characterised profiles had considerable differences from quantitative and qualitative points of view, with a high prevalence of non-terpene hydrocarbons for the EO and oxygenated monoterpenes for both volatiles using SPME and Incigig approaches.

Screening of the compositions of essential oils and volatiles of Perovskia abrotanoides Karel. along with antioxidant, antibacterial and cytotoxic impacts of its methanol extract.

In the present report, a combination of classical and advanced methods, namely classical hydrodistillation (HD) and solvent free microwave extraction (SFME) have been used for the extraction of essential oils from flowers, leaves and stems of Perovskia abrotanoides Karel. as an endemic plant to Golestan Province, Iran. The volatile fractions from the same plant organs have been separated using a solid phase microextraction (SPME) fibre and characterised using a GC/MS apparatus. Moreover, oxygenated monoterpenes, for example, 1,8-cineole and borneol were found as the most constituent components of the majority of the characterised profiles. Accordingly, for flowers, leaves and stems of P. abrotanoides Karel., the relevant quantities were HD: 12.0 and 12.4, 24.0 and 17.9, 12.9 and 16.8%; SFME: 15.6 and 15.2, 20.5 and 16.2, 13.5 and 14.7%; SPME: 20.9 and 5.2, 25.5 and 9.9, 26.4 and 8.5%, respectively. Total phenolic content (TPC: 52.02 mg GAE/g), total flavonoids content (TFC: 98.46 mg QE/g) as well as antioxidant and antibacterial effects of the extract from the aerial parts of this species have been also evaluated. The cytotoxicity of Perovskia abrotanoides Karel. MeOH extract has been tested against HUVECs cell line. Moreover, our study on the in vitro anti-bladder carcinoma demonstrated that the plant extract reduced the viability of malignant bladder cell line, in a dose dependent way.

Profiling of the essential oil compositions from the flowers and leaves of Tanacetum fisherae Aitch. & Hemsl., an endemic plant in Kerman province, Iran.

Tanacetum fisherae Aitch. & Hemsl. is an endemic plant growing wild in some brackish regions of Iran. Since there are not enough reports concerning the quantitative and qualitative analyses of its essential oil, it was decided to characterise the respective water-distilled oils obtained from the flowers and leaves of this medicinal plant. Characterisation of the corresponding essential oil profiles revealed that in both of the analysed oils, oxygenated monoterpenes constituted most of the chemical profiles. In this sense, the most prevailing natural compounds in the flower oils were cis-p-2-menthen-1-ol (11.2%), trans-p-2-menthen-1-ol (10.7%), trans-piperitol (7.8%), 1,8-cineole (6.1%), cis-piperitol (3.8%), α-terpineol (3.7%) and terpinene-4-ol (2.1%), whereas the main constituent components of the leaves oils were, respectively, 1,8-cineole (16.7%), cis-p-2-menthen-1-ol (14.6%), trans-p-2-menthen-1-ol (10.4%), trans-piperitol (12.8%), α-terpineol (5.4%), cis-piperitol (2.9%), borneol (2.7%), and terpinene-4-ol (2.1%). In addition, the second rank of natural compound constituting groups was due to oxygenated sesquiterpenes, as well.[Formula: see text].