Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
J Oral Sci ; 60(1): 163, 2018.
Article in English | MEDLINE | ID: mdl-29576577

ABSTRACT

The publisher regrets that in the above-mentioned article, there was an error in the list of references. The reference numbers "29-32" should be "28-31".

2.
Sci Rep ; 8(1): 414, 2018 01 11.
Article in English | MEDLINE | ID: mdl-29323208

ABSTRACT

Frailty is gaining attention worldwide with the aging of society. Despite the potential lethality and multiple signs and symptoms in affected individuals, preclinical detection of early manifestations leading to frailty syndrome have not been established. We speculated that the composition of the oral microbiota is associated with general frailty, as well as a relationship between gut microbiota and general health condition. In the present study, we investigated the salivary microbiota composition in samples from healthy and frail elderly individuals using 16S rRNA sequencing analysis for characterization. We found a significant difference in diversity between elderly individuals living in a nursing home (EN) and healthy control (HC) subjects, as well as in the microbiota composition at the phyla level. A supervised orthogonal partial least squared discriminant analysis (OPLS-DA) revealed a significant difference in clear classification trend between the EN and HC groups, with all observations falling within the Hotellings T2 (0.95) ellipse, with model fitness parameters of R 2(cum) = 0.937 and Q 2(cum) = 0.888, respectively. In addition, the score plots by unsupervised principal component analysis (PCA) showed a clear classification trend in both groups. Our findings suggest that general frailty is associated with oral microbiota composition and formation.


Subject(s)
Bacteria/classification , Frailty/microbiology , RNA, Ribosomal, 16S/genetics , Saliva/microbiology , Sequence Analysis, DNA/methods , Aged , Aged, 80 and over , Bacteria/genetics , Bacteria/isolation & purification , Case-Control Studies , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , Female , Humans , Male , Microbiota , Nursing Homes , Phylogeny
3.
J Oral Sci ; 59(4): 549-555, 2017 Dec 27.
Article in English | MEDLINE | ID: mdl-28993578

ABSTRACT

Over 700 bacterial species have been detected in the oral cavity. Several studies have suggested that periodontitis is associated with systemic disorders such as diabetes mellitus, indicating a key role for oral microbiota in human health. However, the relationship between oral microbiota and diabetes has not been well clarified. Therefore, we conducted microbiome analysis of saliva samples obtained from 15 elderly residents (3 with type 2 diabetes mellitus [DM] and 12 without diabetes [non-DM]) at three different nursing homes, as well as 9 young healthy controls (HC). Genomic DNA was extracted from each sample, and then the V4 region of the 16S rRNA gene was amplified and sequenced. Alpha diversity, in terms of operational taxonomic unit richness, was significantly higher in samples from the non-DM group than in those from the HC group. Weighted UniFrac distance analysis showed that salivary microbial communities in the DM group were separately clustered. Furthermore, in the DM group, Actinomyces and Selenomonas showed significantly higher abundance, whereas Alloprevotella showed significantly lower abundance, relative to the non-DM group. Although our findings were limited by the small sample size, oral bacterial diversity in the DM group was clearly different from that in the non-DM group.


Subject(s)
Diabetes Mellitus, Type 2/microbiology , Microbiota , Mouth/microbiology , Nursing Homes , Saliva/microbiology , Adult , Aged , Aged, 80 and over , Case-Control Studies , Diet , Female , Health Services Accessibility , Humans , Life Style , Male , Middle Aged , Oral Hygiene , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA
4.
Int J Prosthodont ; 25(4): 368-75, 2012.
Article in English | MEDLINE | ID: mdl-22720288

ABSTRACT

PURPOSE: The aim of this study was to investigate the association of masticatory ability and dental status with intake of fruits and vegetables after adjusting for other factors in independently living elderly Japanese subjects. MATERIALS AND METHODS: The study population consisted of 1,535 community-dwelling, independent elderly subjects over the age of 60 years. Self-assessed general health, financial status, dental status, self-assessed masticatory ability by food acceptance, and frequency of food intake were evaluated from responses to a questionnaire. Multiple logistic regression analysis for shortage of food intake was carried out. RESULTS: Of the participants, 29% had natural dentitions in both the maxilla and mandible and 15% were edentulous in at least one arch and wearing a complete denture. Percentages of participants with nutrient shortages of meat, fish and seafood, green and yellow vegetables, other vegetables, and fruits were 44%, 17%, 30%, 33%, and 12%, respectively. Multiple logistic regression analysis showed that men and subjects with a poor financial status had significant associations with shortages of dietary intake. In addition, shortages of meat, green and yellow vegetables, other vegetables, and fruit were significantly related to poor masticatory ability but not to dental status. Masticatory ability was significantly associated with shortages of green and yellow vegetables. CONCLUSION: Multivariate analyses showed that after adjusting for age, sex, and financial status, self-assessed masticatory ability rather than dental status by itself was significantly associated with shortages in vegetable and fruit intake in independently living elderly Japanese subjects.


Subject(s)
Diet , Fruit , Mastication , Oral Hygiene , Vegetables , Aged , Female , Humans , Japan , Male
SELECTION OF CITATIONS
SEARCH DETAIL
...