ABSTRACT
The effects of fullerene C(60) nanocomposites on human platelet aggregation induced by ADP, ristocetin, and collagen were studied. The nanocomposite containing fullerene C(60) in polyvinyl pyrrolidone solution did not change platelet aggregation, while fullerene C(60) in crown ether and Twin-80 solutions inhibited ADP-induced platelet aggregation by 20 and 30%, respectively.
Subject(s)
Blood Platelets/drug effects , Fullerenes/pharmacology , Nanocomposites/chemistry , Platelet Aggregation/drug effects , Adenosine Diphosphate/pharmacology , Cells, Cultured , Collagen/pharmacology , Crown Ethers/chemistry , Fullerenes/chemistry , Humans , Platelet Aggregation Inhibitors/pharmacology , Povidone/chemistry , Ristocetin/pharmacology , Surface-Active Agents/chemistryABSTRACT
In mammals, nucleus-free erythrocytes participate in two types of movement: a forward and a rotatory motion. The latter type brings about a centrifugal force in the red blood cells. This induces distortion of the erythrocytes, a diffusion speed of the blood components, and an unspecific sorption.
Subject(s)
Erythrocyte Membrane/metabolism , Plasma/metabolism , Absorption , Animals , Centrifugation , Erythrocyte Membrane/drug effects , Ferrosoferric Oxide , Iron/pharmacology , Magnetics , Oxides/pharmacology , Particle Size , Plasma/drug effects , Rabbits , Rotation , Suspensions , Time FactorsSubject(s)
Blood Flow Velocity/radiation effects , Electromagnetic Fields/adverse effects , Erythrocytes/radiation effects , Animals , Blood Flow Velocity/physiology , Blood Sedimentation , Convection , Erythrocytes/physiology , Humans , Microcirculation/physiology , Microcirculation/radiation effects , Microscopy, Video/instrumentation , Microscopy, Video/methods , Periodontium/blood supply , RabbitsABSTRACT
The action of constant current (0.01-3 A, 1-25 V) on human blood during varying exposures induced intensified plaque-formation in the preparations of the local hemolysis test, and transformation of red blood cells-discocytes into echino- and poikilocytes that was attended by pronounced changes in the extent, intensity of light diffusion, natural green and red luminescence of red blood cells caused by flavoproteins and metalloporphyrins. The growth of the last factor has evidenced the presence of significant destructive changes in the cells as a result of constant current direct action on red blood cells.
Subject(s)
Electricity , Erythrocytes/cytology , Erythrocyte Count , Erythrocyte Indices/physiology , Erythrocytes/physiology , Hemolysis/physiology , Humans , In Vitro Techniques , Luminescent Measurements , Male , Spectrophotometry/methods , Time FactorsABSTRACT
Experimentally, the role of photoreactivation in appearance of ultraviolet lesions of corneal epithelium has been studied in white rats. The lesions were induced by ultraviolet radiation, 0.28 mcm wavelength, and intensive (20000 lx) light of a xenon lamp with light filters was used as a reactivating light. The damaging dose as a reactivating light. The damaging dose of ultraviolet radiation by photokeratitis criterion was 11 +/- 2.4 mJ.cm-2. Simultaneous illumination of the animal eye by the damaging ultraviolet light and reactivating radiation exceeded a damaging threshold to 18 +/- 4.0 mJ.cm-2. The results obtained have convincingly shown, that photoreactivation, as a phenomenon, manifested itself in the corneal epithelium and was detected by biomicroscopy of the eye with epithelium staining by a 1% fluorescein solution.
Subject(s)
Cornea/radiation effects , Corneal Diseases/etiology , Light , Radiation Injuries, Experimental/etiology , Ultraviolet Rays/adverse effects , Animals , Dose-Response Relationship, Radiation , RatsABSTRACT
Human and animal cell transformation in the local autoimmune hemolysis and stimulated erythrodieresis is enhanced during plaque formation, which fact is parallelled by a growing number of local hemolysis sites and increase of these sites' area in the preparations. Echino-poikilospherocytic transformation and ultrastructural changes in the red cells in these tests are explained by enhanced synthesis of autoantibodies-hemolysins by autoplaque-forming cells, as well as by the osmotic processes and destabilization of the cellular membrane system, including the leukocytic membranes.
Subject(s)
Erythrocytes , Hemolytic Plaque Technique , Animals , Dogs , Erythrocytes/immunology , Erythrocytes/ultrastructure , Humans , Rabbits , RatsABSTRACT
Transformation and ultrastructure of erythrocytes have been investigated in 58 white non-inbred rats on the 1st, 2nd, 3d days after birth and at the age of 1.5, 3, 8, 20 months. The transformation index of the erythrocytes drops by the 8th month of life and further it rises in old animals. In newborn rats echino-poikiloid forms predominate; they are mainly presented as reticulocytes with remnants of nuclei, mitochondria and other organells. During first three days of life cells of the erythroid line actively free themselves from the nucleus by means of its pyknosis and chromatinolysis. In newborn rats erythrocytes have folds of plasmolemma of linear and spotted form on their surface; their number sharply decreases in mature animals. The least changes in form and ultrastructure of erythrocytes are noticed in 3-8-month-old animals.
Subject(s)
Aging/pathology , Erythrocytes/pathology , Animals , Animals, Newborn , Body Weight , Erythrocytes/ultrastructure , Hemoglobins/analysis , Rats , Reticulocytes/pathologyABSTRACT
Quantitative erythrogram, i. e. the per cent distribution of the cells of different shapes into 5 groups (discocytes, stomatocytes, echinocytes, poikilo- and schizocytes, hemolytic forms) is recommended to be taken into consideration when estimating the red cell transformation in clinical and experimental studies. The classifying morphologic signs of about forty erythrocyte forms are described, and examples of the quantitative erythrogram use are given.
Subject(s)
Erythrocytes, Abnormal/cytology , Animals , Cholecystitis/blood , Guinea Pigs , Hepatitis, Viral, Human/blood , Humans , Male , Myocardial Infarction/blood , RatsABSTRACT
Sensibilization of white rats by horse serum protein has led to quantitative and qualitative changes in internal erythrocyte structure. At the same time changes in leucocyte functional activity and immunocomplex circulation took place. Sensibilization has resulted in the rise of echinocyte, poikilocyte and schizocyte blood level. Sensibilization caused activation of spherulation and partial erythrolysis. The processes are induced by the alterations of cellular ultrastructure.