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1.
Antimicrob Agents Chemother ; 53(5): 1898-906, 2009 May.
Article in English | MEDLINE | ID: mdl-19223628

ABSTRACT

Menstrual toxic shock syndrome is a rare but potentially life-threatening illness manifest through the actions of Staphylococcus aureus toxic shock syndrome toxin 1 (TSST-1). Previous studies have shown that tampon additives can influence staphylococcal TSST-1 production. We report here on the TSST-1-suppressing activity of 34 compounds that are commonly used additives in the pharmaceutical, food, and perfume industries. Many of the tested chemicals had a minimal impact on the growth of S. aureus and yet were potent inhibitors of TSST-1 production. The TSST-1-reducing compounds included surfactants with an ether, amide, or amine linkage to their fatty acid moiety (e.g., myreth-3-myristate, Laureth-3, disodium lauroamphodiacetate, disodium lauramido monoethanolamido, sodium lauriminodipropionic acid, and triethanolamine laureth sulfate); aromatic compounds (e.g. phenylethyl and benzyl alcohols); and several isoprenoids and related compounds (e.g., terpineol and menthol). The membrane-targeting and -altering effects of the TSST-1-suppressing compounds led us to assess the activity of molecules that are known to inhibit fatty acid biosynthesis (e.g., cerulenin, triclosan, and hexachlorophene). These compounds also reduced S. aureus TSST-1 production. This study suggests that more additives than previously recognized inhibit the production of TSST-1.


Subject(s)
Bacterial Toxins , Benzyl Alcohols/pharmacology , Enterotoxins , Fatty Acid Synthesis Inhibitors/pharmacology , Staphylococcus aureus , Superantigens , Surface-Active Agents/pharmacology , Terpenes/pharmacology , Bacterial Toxins/biosynthesis , Benzyl Alcohols/chemistry , Culture Media , Enterotoxins/biosynthesis , Fatty Acid Synthesis Inhibitors/chemistry , Female , Humans , Shock, Septic/microbiology , Staphylococcus aureus/drug effects , Staphylococcus aureus/growth & development , Staphylococcus aureus/metabolism , Superantigens/biosynthesis , Superantigens/drug effects , Surface-Active Agents/chemistry , Terpenes/chemistry , Vagina/microbiology
2.
J Bacteriol ; 187(9): 2967-73, 2005 May.
Article in English | MEDLINE | ID: mdl-15838022

ABSTRACT

Staphylococcal polysaccharide intercellular adhesin (PIA) is important for the development of a mature biofilm. PIA production is increased during growth in a nutrient-replete or iron-limited medium and under conditions of low oxygen availability. Additionally, stress-inducing stimuli such as heat, ethanol, and high concentrations of salt increase the production of PIA. These same environmental conditions are known to repress tricarboxylic acid (TCA) cycle activity, leading us to hypothesize that altering TCA cycle activity would affect PIA production. Culturing Staphylococcus epidermidis with a low concentration of the TCA cycle inhibitor fluorocitrate dramatically increased PIA production without impairing glucose catabolism, the growth rate, or the growth yields. These data lead us to speculate that one mechanism by which staphylococci perceive external environmental change is through alterations in TCA cycle activity leading to changes in the intracellular levels of biosynthetic intermediates, ATP, or the redox status of the cell. These changes in the metabolic status of the bacteria result in the attenuation or augmentation of PIA production.


Subject(s)
Citric Acid Cycle , Polysaccharides, Bacterial/biosynthesis , Staphylococcus epidermidis/metabolism , Acetic Acid/metabolism , Aconitate Hydratase/analysis , Adaptation, Physiological , Biomass , Citrates/pharmacology , Citric Acid Cycle/drug effects , Gene Expression Regulation, Bacterial , Glucose/metabolism , Isocitrate Dehydrogenase/analysis , NAD/analysis , Oxidation-Reduction , Staphylococcus epidermidis/drug effects , Staphylococcus epidermidis/growth & development
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