ABSTRACT
The glutamatergic dysfunction hypothesis suggests genes involved in glutamatergic transmission as candidates for schizophrenia susceptibility genes. We screened single nucleotide polymorphisms (SNPs) in the entire coding sequence of the GluR5 kainate receptor gene, GRIK1, by polymerase chain reaction-single strand conformation polymorphism and direct sequencing. We identified six SNPs including three known ones, 522A/C (174T, synonymous), 1173C/T (391D, synonymous), and 2705C/T (902L/S), as well as three novel ones, 995C/T (332A/V), 2400C/T (800L, synonymous), and 2585A/G (862R/Q). We genotyped Japanese samples of schizophrenia (n = 193-203) and healthy controls (n = 199-215) for three SNPs those were commonly observed in our samples, 522A/C, 1173C/T, and 2705C/T. We observed no significant associations of the SNPs and their haplotypes with schizophrenia. Therefore, we conclude that GRIK1 does not play a major role in schizophrenia pathogenesis in the Japanese population.
Subject(s)
Polymorphism, Single Nucleotide , Receptors, Metabotropic Glutamate/genetics , Schizophrenia/genetics , Base Sequence , DNA Primers , Gene Frequency , Haplotypes , Heterozygote , Homozygote , Humans , Polymerase Chain Reaction , Receptor, Metabotropic Glutamate 5 , Reference ValuesABSTRACT
The motor effective sites of the fifth somatosensory cortex (SV) in the cat were mapped in detail by using unit recording and intracortical microstimulation (ICMS) techniques. The motor effective sites for facial muscle contraction were identified using stimulus currents of less than 30 microA. Of the 257 effective sites detected, 49% were activated by stimulus currents of less than 20 microA and of these, 51% responded to stimulus currents of less than 10 microA. ICMS within the facial area of the SV neuron produced contralateral eye-blinking, the lowest threshold current for which was 2 microA and ICMS within the SV neurons produced whisker movements, the minimum threshold current for which was 4 microA. Furthermore, stimulation of some SV neurons at a threshold current as low as 4 microA produced whisker movements and some responded to both visual and cutaneous stimuli. Ablation of areas 6a beta, 3a, SII, SIII and the motor cortex did not eliminate or reduce the low-threshold responses elicited by this weak stimulation of the SV. These motor effective areas receive input from the contralateral cutaneous of the surrounding muscle motor effective region. Our results suggest that the described effect is independent of motor effective areas.
