ABSTRACT
The composition and the antibacterial activity of the essential oil obtained from Cryptomeria japonica D. Don on oral bacteria were studied. The chemical composition of the essential oil was analysed by GC and GC-MS. Sixty-eight compounds accounting for 95.82% of the oil were identified. The main compounds in the oil were alpha-pinene (6.07%), sabinene (8.86%), terpinen-4-ol (9.77%), alpha-terpineol (6.13%), elemol (11.17%) and 10(15)-cadinen-4-ol (7.16%). The essential oil and some of its major compounds were tested for antimicrobial activity against 15 different genera of oral bacteria. The essential oil of C. japonica exhibited considerable inhibitory effects against all bacteria tested (MICs, 0.025-0.05 mg/mL; MBCs, 0.025-0.1 mg/mL), while its major compounds demonstrated various degrees of growth inhibition.
Subject(s)
Anti-Bacterial Agents/pharmacology , Cryptomeria , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Phytotherapy , Plant Oils/pharmacology , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/therapeutic use , Humans , Microbial Sensitivity Tests , Mouth/microbiology , Plant Leaves , Plant Oils/administration & dosage , Plant Oils/chemistry , Plant Oils/therapeutic useABSTRACT
The chemical components of the essential oil from Artemisia feddei LEV. et VNT. were analyzed using GC-MS. Ninety-nine compounds, accounting for 96.23% of the extracted essential oil, were identified. The main oil compounds were 1,8-cineole (16.86%), chamazulene (9.04%), alpha-terpineol (8.18%), alpha-phellandrene (5.78%), alpha-thujone (5.51%), alpha-terpinyl acetate (5.07%), borneol (5.08%), beta- caryophyllene (4.71%), camphor (4.04%), and terpinen-4-ol (3.04%). The antimicrobial activity of the essential oil and some of its compounds was tested against 15 different genera of oral bacteria. The essential oil from A. feddei had a considerable inhibitory effect on all the obligate anaerobic bacteria tested (MICs, 0.025 to 0.05 mg/ml; MBCs, 0.025 to 0.1mg/ml), whereas the major compounds demonstrated different degrees of growth inhibition.
Subject(s)
Anti-Bacterial Agents/isolation & purification , Artemisia/chemistry , Oils, Volatile/chemistry , Plant Oils/chemistry , Terpenes/isolation & purification , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Gas Chromatography-Mass Spectrometry , Microbial Sensitivity Tests , Oils, Volatile/pharmacology , Optical Rotation , Plant Components, Aerial/chemistry , Plant Oils/pharmacology , Refractometry , Specific Gravity , Terpenes/chemistry , Terpenes/pharmacologyABSTRACT
The chemical components of the essential oil obtained from Artemisia lavandulaefolia DC. were analyzed by GC-MS. Ninety-nine compounds accounting for 94.9 % of the essential oil were identified. The major compounds in the essential oil were beta-caryophyllene (16.1 %), cis-chrysanthenol (7.0 %), 1,8-cineole (5.6 %), borneol (5.3 %), trans-beta-farnesene (5.1 %), camphor (4.9 %), yomogi alcohol (4.5 %), alpha-terpineol (3.9 %), and alpha-humulene oxide (3.3 %). The essential oil and some of its major compounds were tested for antimicrobial activity against 15 different genera of oral bacteria. The essential oil of A. lavandulaefolia exhibited considerable inhibitory effects against all obligate anaerobic bacteria (MIC values, 0.025 to 0.05 mg/mL; MBC values, 0.025 to 0.1 mg/mL) tested, while its major compounds demonstrated different degrees of growth inhibition.
Subject(s)
Anti-Bacterial Agents/pharmacology , Artemisia , Phytotherapy , Plant Oils/pharmacology , Anti-Bacterial Agents/chemistry , Gas Chromatography-Mass Spectrometry , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Humans , Microbial Sensitivity Tests , Plant Oils/chemistryABSTRACT
The chemical composition of the essential oils obtained from Artemisia scoparia Waldst. et Kitamura and Artemisia capillaris Thunb. was analyzed by GC/MS. The essential oil of A. scoparia was rich in camphor (11.0 %), 1,8-cineole (21.5 %), and beta-caryophyllene (6.8 %) as the major compounds, whereas A. capillaris oil was rich in beta-pinene (9.4 %), beta-caryophyllene (11.1 %), and capillene (32.7 %). The essential oils and some of their major compounds were tested for their antimicrobial activity against 15 different genera of oral bacteria. The essential oils of A. scoparia and A. capillaris exhibited considerable inhibitory effects against all oral bacteria tested, while their major components demonstrated various degrees of growth inhibition.
Subject(s)
Anti-Bacterial Agents/pharmacology , Artemisia , Phytotherapy , Plant Oils/pharmacology , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/therapeutic use , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Humans , Microbial Sensitivity Tests , Plant Components, Aerial , Plant Oils/administration & dosage , Plant Oils/chemistry , Plant Oils/therapeutic useABSTRACT
The essential oil of Chrysanthemum boreale Makino was analyzed by means of GC and GC-MS. Eighty-seven constituents were identified, representing 94.13 % of the total oil and the major components were camphor, alpha-thujone, cis-chrysanthenol, 1,8-cineole, alpha-pinene, and beta-caryophyllene. Furthermore, the essential oil exhibited antibacterial activity (MIC, more than 800 microg/mL versus 0.125 microg/mL for ampicillin) after it was tested against 6 Gram(+) bacteria and 8 Gram(-) bacteria.
Subject(s)
Anti-Bacterial Agents/pharmacology , Chrysanthemum , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Phytotherapy , Plant Oils/pharmacology , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/therapeutic use , Humans , Microbial Sensitivity Tests , Plant Oils/chemistry , Plant Oils/therapeutic useABSTRACT
The chemical composition of the essential oil from Artemisia iwayomogi Kitamura was analyzed by means of GC and GC-MS. Eighty-five constituents were identified representing 96.23 % of the total oil. Camphor (19.31 %), 1,8-cineole (19.25 %), borneol (18.96 %), camphene (4.64 %), and beta-caryophyllene (3.46 %) were found to be the major components. Furthermore, the oil exhibited antibacterial activity against six Gram-(+) and six Gram-(-) bacteria in tests using the broth dilution method.