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Exp Neurol ; 347: 113902, 2022 01.
Article in English | MEDLINE | ID: mdl-34699789

ABSTRACT

Immunometabolic changes have been shown to be a key factor in determining the immune cell response in disease models. The immunometabolite, itaconate, is produced by aconitate decarboxylase 1 (Acod1) and has been shown to inhibit inflammatory signaling in macrophages. In this study, we explore the role of Acod1 and itaconate in cerebral ischemia/reperfusion injury. We assessed the effect of global Acod1 knockout (Acod1KO, loss of endogenous itaconate) in a transient ischemia/reperfusion occlusion stroke model. Mice received a transient 90-min middle cerebral artery occlusion followed with 24-h of reperfusion. Stroke lesion volume was measured by MRI analysis and brain tissues were collected for mRNA gene expression analysis. Acod1KO mice showed significant increases in lesion volume compared to control mice, however no differences in pro-inflammatory mRNA levels were observed. Cell specific knockout of Acod1 in myeloid cells (LysM-Cre), microglia cells (CX3CR1, Cre-ERT2) and Endothelial cells (Cdh5(PAC), Cre-ERT2) did not reproduce lesion volume changes seen in global Acod1KO, indicating that circulating myeloid cells, resident microglia and endothelial cell populations are not the primary contributors to the observed phenotype. These effects however do not appear to be driven by changes in inflammatory gene regulation. These data suggests that endogenous Acod1 is protective in cerebral ischemia/reperfusion injury.


Subject(s)
Brain Ischemia/enzymology , Brain Ischemia/prevention & control , Carboxy-Lyases/deficiency , Reperfusion Injury/enzymology , Reperfusion Injury/prevention & control , Animals , Brain Ischemia/genetics , Carboxy-Lyases/genetics , Cell Line , Laser-Doppler Flowmetry/methods , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Reperfusion Injury/genetics
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