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1.
Opt Express ; 28(26): 38942-38948, 2020 Dec 21.
Article in English | MEDLINE | ID: mdl-33379452

ABSTRACT

A novel tunable transmitter structure based on liquid crystal filter, to the best of our knowledge, is presented. The structure is designed for application to 5G fronthaul and supports 25 Gbps dense wavelength division multiplexing (WDM) transmission and tunable range of 35 nm. The design takes into account easy change of operation band over coarse WDM grid. Prototype samples are developed to test feasibility of the design.

2.
Transplant Proc ; 50(10): 3984-3987, 2018 Dec.
Article in English | MEDLINE | ID: mdl-30577301

ABSTRACT

Brain death is a rare situation after living-donor liver transplantation. However, the recipient who suffers from brain death and has functional liver graft is a potential liver donor. We report the 1st case of successful reuse of extended right living-donor liver graft after brain death of the first recipient. The first recipient, who had acute liver failure caused by hepatitis A virus, experienced brain death on the 2nd day after the transplantation. The allograft had a favorable regeneration and functional recovery. On the 7th day, the allograft was procured with a patent hepatic artery, bile duct, portal vein, and reconstructed outflow (right hepatic vein and middle hepatic vein) and successfully implanted into the second recipient. The second recipient has experienced a long-term survival without any complications.


Subject(s)
Liver Transplantation/mortality , Liver Transplantation/methods , Living Donors/supply & distribution , Tissue and Organ Procurement/methods , Adult , Brain Death , Female , Humans , Male , Treatment Outcome
3.
Clin Transl Oncol ; 20(8): 1011-1017, 2018 Aug.
Article in English | MEDLINE | ID: mdl-29256155

ABSTRACT

PURPOSE: We investigated the role of adjuvant concurrent chemoradiation therapy (CCRT) in patients with a microscopically positive resection margin (R1) after curative resection for extrahepatic cholangiocarcinoma (EHCC). METHODS/PATIENTS: A total of 84 patients treated with curative resection for EHCC were included. Fifty-two patients with negative resection margins did not receive any adjuvant treatments (R0 + S group). The remaining 32 patients with microscopically positive resection margins received either adjuvant CCRT (R1 + CCRT group, n = 19) or adjuvant radiation therapy (RT) alone (R1 + RT group, n = 13). RESULTS: During the median follow-up period of 26 months, the 2-year locoregional recurrence-free survival (LRRFS), disease-free survival (DFS), and overall survival rates (OS) were: 81.8, 62.6, and 61.5% for R0 + S group; 71.8, 57.8, and 57.9% for R1 + CCRT group; and 16.8, 9.6, and 15.4% for R1 + RT group, respectively. Multivariate analysis revealed that the R1 + CCRT group did not show any significant difference in survival rates compared with the R0 + S group. The R1 + RT group had lower LRRFS [hazard ratio (HR) 3.008; p = 0.044], DFS (HR 2.364; p = 0.022), and OS (HR 2.417; p = 0.011) when compared with the R0 + S and R1 + CCRT group. CONCLUSIONS: A lack of significant survival difference between R0 + S group and R1 + CCRT group suggests that adjuvant CCRT ameliorates the negative effect of microscopic positive resection margin. In contrast, adjuvant RT alone is appeared to be inadequate for controlling microscopically residual tumor.


Subject(s)
Bile Duct Neoplasms/therapy , Chemoradiotherapy, Adjuvant/mortality , Chemoradiotherapy/mortality , Cholangiocarcinoma/therapy , Neoplasm Recurrence, Local/therapy , Neoplasm, Residual/therapy , Adult , Aged , Aged, 80 and over , Bile Duct Neoplasms/pathology , Bile Duct Neoplasms/surgery , Cholangiocarcinoma/pathology , Cholangiocarcinoma/surgery , Combined Modality Therapy , Female , Follow-Up Studies , Humans , Male , Middle Aged , Neoplasm Recurrence, Local/pathology , Neoplasm, Residual/pathology , Prognosis , Retrospective Studies , Survival Rate
4.
Transplant Proc ; 49(10): 2395-2398, 2017 Dec.
Article in English | MEDLINE | ID: mdl-29198688

ABSTRACT

INTRODUCTION: Although outcomes of liver transplantation (LT) have improved as the result of progress in surgical procedures, a failure to restore sufficient graft outflow may yield fatal consequences including graft dysfunction and even graft loss to date. In particular, patients with pre-existing obliterated venous drainage, such as those with Budd-Chiari syndrome (BCS), are at high risk of having venous complications followed by conventional LT. In selected cases, the transplant surgeons are compelled to modify the surgical procedures of LT from the conventional procedure. METHODS: We describe an LT performed in a BCS patient with complete inferior vena cava (IVC) obstruction. A procedure that we named "hanging hepato-atrial anastomosis" was performed, in which 2 major modifications were made. One modification was the dissection of the lower inlet of the right atrium by use of a trans-abdominal approach and hepato-atrial anastomosis. This was performed by exposing the thoracic IVC through a trans-abdominal approach. The other modification was the manufacture of a blind pouch from the graft's infra-hepatic IVC without anastomosis. RESULTS: Modifications were made possible as the result of meticulous examination of the patient's vascular anatomy before the operation. Fortunately, the patient had a heavy network of pre-vertebral veins that drained blood from the lower extremity and both kidneys to the azygos-hemi-azygos veins. CONCLUSIONS: We learned that a meticulous assessment of vascular anatomy and complete understanding of hemodynamics are the keys to the successful LT for BCS in patients with extensive IVC abnormality. Thoracotomy may not be necessary to explore thoracic IVC when performing hepato-atrial anastomosis in LT for BCS.


Subject(s)
Budd-Chiari Syndrome/surgery , Liver Transplantation/methods , Anastomosis, Surgical/methods , Hepatic Veins/surgery , Humans , Male , Middle Aged , Vena Cava, Inferior/surgery
5.
Oncogene ; 36(3): 332-349, 2017 01 19.
Article in English | MEDLINE | ID: mdl-27345400

ABSTRACT

Studies have reported that interactions between keratins (KRTs) and other proteins initiate signaling cascades that regulate cell migration, invasion, and metastasis. In the current study, we found that expression of KRT19 was specifically high in breast cancers and significantly correlated with their invasiveness. Moreover, knockdown of KRT19 led to increased proliferation, migration, invasion, drug resistance, and sphere formation in breast cancer cells via an upregulated NOTCH signaling pathway. This was owing to reduced expression of NUMB, an inhibitory protein of the NOTCH signaling pathway. In addition, we found that KRT19 interacts with ß-catenin/RAC1 complex and enhances the nuclear translocation of ß-catenin. Concordantly, knockdown of KRT19 suppressed the nuclear translocation of ß-catenin as well as ß-catenin-mediated NUMB expression. Furthermore, modulation of KRT19-mediated regulation of NUMB and NOTCH1 expression led to the repression of the cancer stem cell properties of breast cancer patient-derived CD133high/CXCR4high/ALDH1high cancer stem-like cells (CSLCs), which showed very low KRT19 and high NOTCH1 expression. Taken together, our study suggests a novel function for KRT19 in the regulation of nuclear import of the ß-catenin/RAC1 complex, thus modulating the NUMB-dependent NOTCH signaling pathway in breast cancers and CSLCs, which might bear potential clinical implications for cancer or CSLC treatment.


Subject(s)
Breast Neoplasms/pathology , Keratin-19/metabolism , Membrane Proteins/metabolism , Nerve Tissue Proteins/metabolism , Receptors, Notch/metabolism , beta Catenin/metabolism , rac1 GTP-Binding Protein/metabolism , Animals , Breast Neoplasms/genetics , Breast Neoplasms/metabolism , Cell Line, Tumor , Cell Movement , Cell Nucleus/metabolism , Cell Proliferation , Female , Gene Expression Regulation, Neoplastic , Gene Knockdown Techniques , Hep G2 Cells , Humans , Keratin-19/genetics , MCF-7 Cells , Mice , Neoplasm Invasiveness , Neoplasm Transplantation , Signal Transduction , Up-Regulation
6.
Transplant Proc ; 48(3): 749-55, 2016 Apr.
Article in English | MEDLINE | ID: mdl-27234728

ABSTRACT

BACKGROUND: The CD207 molecule, also known as langerin, is known to be the first immunologic barrier expressed on Langerhans cells, which are the immature dendritic cells of the epidermis and mucosa. Inflammation could be a causal factor in the progression of chronic kidney disease. To date, convincing experimental and clinical evidence indicates that langerin plays a role in antigen uptake and processing, which is the primary function of Langerhans cells. In this study, we hypothesized that polymorphisms of the CD207 (Langerin) gene may be associated with end-stage renal disease (ESRD). METHODS: To evaluate whether an association exists between CD207 and ESRD susceptibility, we assessed the relation between 10 exonic single-nucleotide polymorphisms (SNPs) in the CD207 gene and ESRD in 90 kidney transplant patients and 545 control subjects. RESULTS: Compared with the control participants, the frequencies of the CD207 rs13421115*T and rs17718987*C alleles were significantly higher in the patients with ESRD. Genotyping analysis demonstrated that 2 SNPs (rs13421115 and rs17718987) included in the study were significantly associated with ESRD in the codominant1 (rs13421115; Fisher exact P = .027), dominant (rs13421115; Fisher exact P = .004; and rs17718987; Fisher exact P = .004), and log-additive (rs13421115; Fisher exact P = .004; and rs17718987; Fisher exact P = .004) models after adjusting for age and sex. CONCLUSIONS: We suggest that CD207 gene polymorphisms rs13421115 and rs17718987 increase the risk of development of ESRD.


Subject(s)
Antigens, CD/genetics , Genetic Predisposition to Disease , Kidney Failure, Chronic/genetics , Lectins, C-Type/genetics , Mannose-Binding Lectins/genetics , Polymorphism, Single Nucleotide , Adolescent , Adult , Aged , Alleles , Case-Control Studies , Child , Female , Genotype , Humans , Male , Middle Aged , Republic of Korea , Young Adult
8.
Int J Impot Res ; 28(1): 25-30, 2016.
Article in English | MEDLINE | ID: mdl-26510966

ABSTRACT

Udenafil is a selective phosphodiesterase type 5 inhibitor made available in recent years for the treatment of erectile dysfunction. Herein, we evaluated independent predictors of potency recovery in radical prostatectomy (RP) patients who underwent penile rehabilitation with udenafil 50 mg. One hundred and forty-three men who underwent RP were enrolled in a penile rehabilitation program using udenafil 50 mg every other day. The rate of regained potency in the study group was significantly higher compared with the recovery rate seen in patients who were not part of the penile rehabilitation program (41.3% vs 13.0%; P<0.001). On the multivariate Cox analyses, preoperative International Index of Erectile Function-5 scores (hazard ratio (HR), 1.049; P=0.040), alcohol consumption (HR, 2.043; P=0.020) and Gleason biopsy score (HR, 0.368; P=0.024) were independent preoperative predictors for potency recovery. Among post-RP variables, the use of robotic procedures (HR, 2.287; P=0.030) and pathologic stage (HR, 0.506; P=0.038) were significantly associated with potency recovery. This study identified predictive factors for the recovery of potency in patients undergoing penile rehabilitation with udenafil following RP. Our results could provide physicians with useful information for counseling RP patients and selecting optimal candidates for penile rehabilitation.


Subject(s)
Erectile Dysfunction , Postoperative Complications , Prostatectomy/adverse effects , Pyrimidines/administration & dosage , Sulfonamides/administration & dosage , Aged , Erectile Dysfunction/drug therapy , Erectile Dysfunction/etiology , Erectile Dysfunction/physiopathology , Erectile Dysfunction/rehabilitation , Humans , Male , Middle Aged , Phosphodiesterase 5 Inhibitors/administration & dosage , Postoperative Complications/drug therapy , Postoperative Complications/physiopathology , Postoperative Complications/rehabilitation , Prognosis , Prostatectomy/methods , Prostatic Neoplasms/surgery , Recovery of Function/drug effects , Recovery of Function/physiology , Republic of Korea , Risk Factors , Treatment Outcome
9.
Genet Mol Res ; 14(3): 7502-18, 2015 Jul 06.
Article in English | MEDLINE | ID: mdl-26214429

ABSTRACT

Understanding genetic diversity, population structure, and linkage disequilibrium is a prerequisite for the association mapping of complex traits in a target population. In this study, the genetic diversity and population structure of 40 waxy and 40 normal inbred maize lines were investigated using 10 morphological traits and 200 simple sequence repeat (SSR) markers. Based on a population structure analysis, the 80 maize inbred lines were divided into three groups: I, II, and admixed. Significant marker-trait associations were identified between the markers and the 10 morphological traits, which were studied according to the model used to confirm the association. Using a general linear model, the lowest R(2) value (9.03) was detected in umc1139, which was associated with ear number, and the highest (43.97) was in umc1858, which was associated with plant height. Using a mixed linear model, the lowest R(2) value (18.74) was in umc1279, which was associated with ear weight; the highest (27.66) was in umc1858, which was associated with 100-kernel weight. The SSR markers identified in the present study may serve as useful molecular markers for selecting important yield and agronomic traits. These results will be useful for marker-assisted selection in maize breeding programs, to help breeders choose parental lines and markers for crosses.


Subject(s)
Chromosome Mapping , Genetic Variation , Inbreeding , Quantitative Trait, Heritable , Waxes/metabolism , Zea mays/genetics , Alleles , Cluster Analysis , Gene Frequency/genetics , Genetic Markers , Genetics, Population , Microsatellite Repeats/genetics , Models, Genetic , Phenotype , Phylogeny
10.
Clin Radiol ; 70(6): 638-43, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25824279

ABSTRACT

AIM: To evaluate the frequency of thyroglossal duct remnant (TGDR) uptake on post-therapy (131)I-scintigraphy in thyroid cancer patients, to analyse the rate of persistent TGDR uptake on follow-up combined (131)I-single-photon emission CT/CT (SPECT/CT), and to identify the differential clinical characteristics between patients with positive and negative TGDR uptake on (131)I-SPECT/CT. MATERIALS AND METHODS: A total of 179 patients treated with total thyroidectomy for thyroid cancer were enrolled in the study. At (131)I-whole-body scan (WBS), TGDR uptake was defined as an increase in radioactivity at the midline of the neck versus the thyroid bed. TGDR uptake on (131)I- SPECT/CT was defined as the presence of radioactivity at the expected pathway of the thyroglossal duct without evidence of metastatic foci. Persistent TGDR uptake was confirmed when TGDR uptake on follow-up (131)I-SPECT/CT corresponded to previous TGDR uptake detected by post-therapy (131)I-SPECT/CT. RESULTS: At SPECT/CT, TGDR uptake was noted in 86 of 179 patients. Stimulated thyroglobulin (sTg) levels were significantly higher (p = 0.02) in patients with positive TGDR uptake. Persistent TGDR uptake on follow-up (131)I-SPECT/CT was noted in 15 of 86 patients; sTg levels were significantly higher (p = 0.03) in the patients with persistent TGDR uptake. CONCLUSION: TGDR uptake is frequently visualized on post-therapy (131)I-SPECT/CT images and can be resistant to (131)I ablation. TGDR uptake has the potential to result in an elevation of serum thyroglobulin levels. (131)I-SPECT/CT clarifies TGDR uptake without additional invasive procedures or imaging studies, eliminating confusion among clinicians for managing differentiated thyroid cancer patients.


Subject(s)
Thyroid Neoplasms/diagnostic imaging , Tomography, Emission-Computed, Single-Photon/methods , Adolescent , Adult , Aged , Catheter Ablation/methods , Female , Humans , Iodine Radioisotopes , Male , Middle Aged , Neoplasm, Residual , Radiopharmaceuticals , Thyroglossal Cyst/diagnostic imaging , Thyroid Neoplasms/surgery , Thyroidectomy/methods , Young Adult
11.
Transplant Proc ; 47(1): 161-4, 2015.
Article in English | MEDLINE | ID: mdl-25645797

ABSTRACT

A 37-year-old man with moderately severe hemophilia A (factor VIII of 1.2%), who had a normal liver without liver cirrhosis or hepatocellular carcinoma, was referred to our liver transplantation (LT) team. LT was planned for sufficient coagulation factor level maintenance and prophylaxis against future hemorrhagic complications. The donor was the patient's 35-year-old wife, who was nonhemophilic. We performed an auxiliary partial orthotopic liver transplantation (APOLT) with the approval of the Institutional Ethics Committee. A left partial liver graft taken from the donor was orthotopically transplanted to the recipient after resection of the native left hemiliver while preserving the native right lobe. After surgery, the patient tolerated the procedure, and tacrolimus was used to maintained immunosuppression. In this recipient, factor VIII activity significantly increased soon after the APOLT, and has been maintained at >20% without any further bleeding episodes for the past 74 months since the procedure. This finding suggests that APOLT may be an effective alternative treatment for patients with hemophilia A.


Subject(s)
Hemophilia A/surgery , Liver Transplantation/methods , Adult , Hepatectomy , Humans , Male , Treatment Outcome
12.
Environ Monit Assess ; 187(1): 4192, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25519872

ABSTRACT

Time series analyses (autocorrelation, spectral density, and cross-correlation) and principal component analysis (PCA) were used to understand the characteristics of the selected hydrochemical parameters pH, turbidity, alkalinity, Cl, hardness, total dissolved solids (TDS), and metals Fe and Mn in the Nakdong River, South Korea. Autocorrelation and spectral density for pH, alkalinity, hardness, and Cl were very similar to TDS, whereas Fe, Mn, and turbidity showed different trends from TDS. Cross-correlograms of pH, alkalinity, hardness, and Cl versus TDS were very similar to each other. Those of Fe and turbidity represented the opposite relations with other components. Cross-correlation coefficients had the highest values at zero lag, indicating that pH, alkalinity, hardness, and Cl are controlling factors for TDS. On the other hand, Fe and turbidity showed the highest values at 6-month lag and Mn at a month lag. PCA indicated that TDS had very close relation with hardness, pH, and Cl and very small relation with Mn. Turbidity and Fe had relatively opposite relations with TDS. It was concluded that the geostatistical methods were very useful for evaluating the hydrochemical characteristics of the Nakdong River water in South Korea.


Subject(s)
Environmental Monitoring/methods , Rivers/chemistry , Water Pollutants/analysis , Water Pollution/statistics & numerical data , Principal Component Analysis , Republic of Korea
13.
Br J Radiol ; 87(1041): 20140347, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25027034

ABSTRACT

OBJECTIVE: To evaluate the safety and feasibility of N-butyl cyanoacrylate (N-BCA) embolization of percutaneous transhepatic portal venous access tract and to establish an appropriate technique. METHODS: 40 consecutive patients underwent percutaneous transhepatic portal venous intervention for various reasons. Embolization of percutaneous transhepatic portal venous access tract was performed after the procedure in all of the patients using N-BCA and Lipiodol® (Lipiodol Ultra Fluide; Laboratoire Guerbet, Aulnay-sous-Bois, France) mixture. Immediate ultrasonography and fluoroscopy were performed to evaluate perihepatic haematoma formation and unintended embolization of more than one segmental portal vein. Follow-up CT was performed, and haemoglobin and haematocrit levels were checked to evaluate the presence of bleeding. RESULTS: Immediate haemostasis was achieved in all of the patients, without development of perihepatic haematoma or unintended embolization of more than one segmental portal vein. Complete embolization of percutaneous access tract was confirmed in 39 out of 40 patients by CT. Seven patients showed decreased haemoglobin and haematocrit levels. Other complications included mild pain at the site of embolization and mild fever, which resolved after conservative management. 16 patients died during the follow-up period owing to progression of the underlying disease. CONCLUSION: Embolization of percutaneous transhepatic portal vein access tract with N-BCA is feasible and technically safe. With the appropriate technique, N-BCA can be safely used as an alternate embolic material since it is easy to use and inexpensive compared with other embolic materials. ADVANCES IN KNOWLEDGE: This is the first study to investigate the efficacy of N-BCA for percutaneous transhepatic portal venous access tract embolization.


Subject(s)
Embolization, Therapeutic/methods , Enbucrilate/therapeutic use , Portal Vein , Adult , Aged , Contrast Media , Ethiodized Oil , Feasibility Studies , Female , Fluoroscopy , Hemostasis, Surgical , Humans , Male , Middle Aged , Treatment Outcome
14.
Minerva Urol Nefrol ; 66(3): 157-64, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25072130

ABSTRACT

AIM: We present the oncologic outcomes of laparoscopic nephroureterectomy management of pT3 upper urinary tract urothelial carcinoma. METHODS: Between October 2003 and January 2011, 50 patients with pT3 upper urinary tract urothelial carcinoma which had pathologically confirmed underwent laparoscopic nephroureterectomy at our institution. Demographic data, perioperative results, pathological findings and oncologic outcomes were reviewed and analyzed retrospectively. RESULTS: There were 36 patients (72%) of high grade lesion and 14 patients (28%) of low grade lesion. Lymphovascular invasion was observed in 16 patients (32%) and the surgical margin was positive in one patient. N stage was pN0 in 16 (32%), pN1 in 3 (6%), pN2 in 1 (2%) and pN3 in 1 (2%). The 5-year overall survival rate was 52.6% and the 5-year cancer-specific survival rate was 65.3%. Overall recurrence developed in 23 patients. There were 10 patients (20%) of urothelial recurrence which were all occurred in the bladder at the mean period of 13.6 months, and 7 patients of them were invasive bladder cancer. There were 16 patients (32%) of non-urothelial recurrence developed at the mean period of 9.69 months. On multivariate analyses lymphadenopathy and lymph node involvement of cancer (N+) were identified as independent predictive factors for the cancer-specific survival, and concomitant bladder tumor, grade and lymphovascular invasion were identified as independent predictive factors for the overall recurrence free survival. CONCLUSION: Laparoscopic nephroureterectomy in patients with high stage upper urinary tract urothelial carcinoma appear comparable to those of open surgery in the regard of oncologic outcomes.


Subject(s)
Carcinoma, Transitional Cell/surgery , Laparoscopy/statistics & numerical data , Nephrectomy/methods , Ureter/surgery , Urologic Neoplasms/surgery , Aged , Carcinoma, Transitional Cell/pathology , Carcinoma, Transitional Cell/secondary , Disease-Free Survival , Female , Humans , Kaplan-Meier Estimate , Lymphatic Metastasis , Male , Middle Aged , Neoplasm Grading , Neoplasm Invasiveness , Neoplasm Staging , Neoplasms, Multiple Primary/surgery , Nephrectomy/statistics & numerical data , Proportional Hazards Models , Retrospective Studies , Treatment Outcome
15.
Anim Genet ; 45(3): 442-4, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24506094

ABSTRACT

Growth-related traits are complex and economically important in the livestock industry. The aim of this study was to identify quantitative trait loci (QTL) and the associated positional candidate genes affecting growth in pigs. A genome-wide association study (GWAS) was performed using the porcine single-nucleotide polymorphism (SNP) 60K bead chip. A mixed-effects model and linear regression approach were used for the GWAS. The data used in the study included 490 purebred Landrace pigs. All experimental animals were genotyped with 39 438 SNPs located throughout the pig autosomes. We identified a strong association between a SNP marker on chromosome 16 and body weight at 71 days of age (ALGA0092396, P = 5.35 × 10(-9) , Bonferroni adjusted P < 0.05). The SNP marker was located near the genomic region containing IRX4, which encodes iroquois homeobox 4. This SNP marker could be useful in the selective breeding program after validating its effect on other populations.


Subject(s)
Genome-Wide Association Study/veterinary , Polymorphism, Single Nucleotide , Quantitative Trait Loci/genetics , Sus scrofa/growth & development , Sus scrofa/genetics , Animals , Female , Male , Oligonucleotide Array Sequence Analysis/veterinary
16.
Asian-Australas J Anim Sci ; 26(7): 1047-53, 2013 Jul.
Article in English | MEDLINE | ID: mdl-25049884

ABSTRACT

Five hundred and forty crossbred (Korean native black pig×Landrace) F2 were selected at a commercial pig farm and then divided into six different coat color groups: (A: Black, B: White, C: Red, D: White spot in black, E: Black spot in white, F: Black spot in red). Birth weight, 21st d weight, 140th d weight and carcass weight varied among the different coat color groups. D group (white spot in black coat) showed a significantly higher body weight at each weigh (birth weight, 140th d weight and carcass weight) than did the other groups, whereas the C group (red coat color) showed a significantly lower body weight at finishing stage (140th d weight and carcass weight) compared to other groups. Meat quality characteristics, shear force, cooking loss and meat color were not significantly different among the different coat color groups, whereas drip loss was significantly higher in F than in other groups. Most blood characteristics were not significantly different among the different groups, except for the red blood cells.

17.
Anim Genet ; 42(4): 451-5, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21749430

ABSTRACT

The KIT locus has been suggested to be a strong candidate region linked with whole-body roan in the F(2) population produced by intercrosses between Landrace and Korean Native pigs. In this manuscript, we report the finding of a novel alternative splicing event in the porcine KIT gene that results in the skipping of exon 5 in the I(Rn) allele. KIT mRNAs that lack exon 5 were identified in the large intestine and skin, suggesting that the mechanism responsible for the skipping of exon 5 may be tissue specific. A U(26) repeat in intron 5 showed complete linkage (LOD = 11.8) with the roan phenotype and absolute association with the black phenotype of the Korean Native pig (KNP) population samples, inferring that the repeat pattern may alter the complementary base-pairing-mediated looping-out of introns 4 and 5, which may mediate the exon 5-skipping event. Although the sample size in our study was relatively small, we speculate that the R3 allele containing the U(26) repeat is a causative element for the roan phenotype via alternative control of the exon skipping in our roan pedigree.


Subject(s)
Alternative Splicing/genetics , Exons/genetics , Hair/physiology , Pigmentation/genetics , Proto-Oncogene Proteins c-kit/genetics , RNA, Messenger/genetics , Sus scrofa/genetics , Animals , Base Sequence , Crosses, Genetic , DNA Primers/genetics , Genetic Linkage , Genetics, Population , Molecular Sequence Data , Pedigree , Pigmentation/physiology , Republic of Korea , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNA , Sus scrofa/physiology
19.
Transplant Proc ; 42(7): 2563-6, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20832544

ABSTRACT

Hepatorenal syndrome (HRS) is a reversible, functional renal failure that occurs in patients with advanced hepatic failure. However, the reported rates of complete recovery of renal function and patient survivals after orthotopic liver transplantation (OLT) are variable. The aim of this study was to compare the outcomes after OLT between patients with HRS and those without HRS (no-HRS). We established exclusion criteria to select study patients who underwent OLT in a single center between January 2005 and October 2008. The exclusion criteria included the following: (1) malignancy, (2) <18 years of age, (3) other than primary OLT, (4) ABO mismatch or hemophilia, (5) no liver cirrhosis, and (6) survival >1 month after OLT. We selected 71 subjects, including 8 HRS and 63 no-HRS patients. No significant differences were observed in the estimated glomerular filtration rate (eGFR) between the 2 groups except for a lower eGFR on the day of and 1 month after OLT in the HRS group: 108.3 ± 40.5 versus 31.4 ± 14.1 mL/min and 85.4 ± 15.0 versus 57.3 ± 12.1 mL/min (P = .000 and P = .014, respectively). The renal function of 6/7 HRS patients who survived >1 year improved. The 1-year patient survival rate after OLT in HRS patients was similar to that without HRS: 95% versus 86% (P = .37). We concluded that HRS had minimal effects on patient survival and return of acceptable renal function.


Subject(s)
Hepatorenal Syndrome/physiopathology , Liver Transplantation/adverse effects , Adult , Creatinine/blood , Female , Glomerular Filtration Rate , Hepatorenal Syndrome/mortality , Hepatorenal Syndrome/surgery , Humans , International Normalized Ratio , Kidney Transplantation/mortality , Kidney Transplantation/physiology , Liver Cirrhosis/etiology , Liver Cirrhosis/surgery , Liver Function Tests , Liver Transplantation/mortality , Male , Middle Aged , Patient Selection , Prothrombin Time , Survival Rate
20.
Cell Death Differ ; 17(8): 1254-65, 2010 Aug.
Article in English | MEDLINE | ID: mdl-20139895

ABSTRACT

Lipid rafts have been known to be platforms to initiate cellular signal transduction of insulin-like growth factor (IGF) inducing skeletal muscle differentiation and hypertrophy. Here, tripartite motif 72 (TRIM72), with a really interesting new gene (RING)-finger domain, a B-box, two coiled-coil domains, and a SPRY (SPla and RYanodine receptor) domain, was revealed to be predominantly expressed in the sarcolemma lipid rafts of skeletal and cardiac muscles. Adenoviral TRIM72 overexpression prevented but RNAi-mediated TRIM72 silencing enhanced C2C12 myogenesis by modulating the IGF-induced insulin receptor substrate-1 (IRS-1) activation through the molecular association of TRIM72 with IRS-1. Furthermore, myogenic activity was highly enhanced with increased IGF-induced Akt activation in the satellite cells of TRIM72(-/-) mice, compared to those of TRIM72+/+ mice. Because TRIM72 promoter analysis shows that two proximal E-boxes in TRIM72 promoter were essential for MyoD- and Akt-dependent TRIM72 transcription, we can conclude that TRIM72 is a novel antagonist of IRS-1, and is essential as a negative regulator of IGF-induced muscle differentiation.


Subject(s)
Carrier Proteins/metabolism , Insulin Receptor Substrate Proteins/metabolism , Muscle Development , Muscle, Skeletal/physiology , Animals , Carrier Proteins/antagonists & inhibitors , Carrier Proteins/genetics , Cell Differentiation , Cell Line , Female , Male , Membrane Microdomains/metabolism , Membrane Proteins , Mice , Proto-Oncogene Proteins c-akt/metabolism , RNA Interference , RNA, Small Interfering/metabolism , Satellite Cells, Skeletal Muscle/cytology , Satellite Cells, Skeletal Muscle/metabolism , Signal Transduction
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