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1.
Autophagy ; 20(2): 365-379, 2024 02.
Article in English | MEDLINE | ID: mdl-37712850

ABSTRACT

Cerebral ischemia induces massive mitochondrial damage, leading to neuronal death. The elimination of damaged mitochondria via mitophagy is critical for neuroprotection. Here we show that the level of PA2G4/EBP1 (proliferation-associated 2G4) was notably increased early during transient middle cerebral artery occlusion and prevented neuronal death by eliciting cerebral ischemia-reperfusion (IR)-induced mitophagy. Neuron-specific knockout of Pa2g4 increased infarct volume and aggravated neuron loss with impaired mitophagy and was rescued by introduction of adeno-associated virus serotype 2 expressing PA2G4/EBP1. We determined that PA2G4/EBP1 is ubiquitinated on lysine 376 by PRKN/PARKIN on the damaged mitochondria and interacts with receptor protein SQSTM1/p62 for mitophagy induction. Thus, our study suggests that PA2G4/EBP1 ubiquitination following cerebral IR-injury promotes mitophagy induction, which may be implicated in neuroprotection.Abbreviations: AAV: adeno-associated virus; ACTB: actin beta; BNIP3L/NIX: BCL2 interacting protein 3 like; CA1: Cornu Ammonis 1; CASP3: caspase 3; CCCP: carbonyl cyanide m-chlorophenyl hydrazone; DMSO: dimethyl sulfoxide; PA2G4/EBP1: proliferation-associated 2G4; FUNDC1: FUN14 domain containing 1; IB: immunoblotting; ICC: immunocytochemistry; IHC: immunohistochemistry; IP: immunoprecipitation; MCAO: middle cerebral artery occlusion; MEF: mouse embryonic fibroblast; OGD: oxygen-glucose deprivation; PRKN/PARKIN: parkin RBR E3 ubiquitin protein ligase; PINK1: PTEN induced kinase 1; RBFOX3/NeuN: RNA binding fox-1 homolog 3; SQSTM1/p62: sequestosome 1; TIMM23: translocase of inner mitochondrial membrane 23; TOMM20: translocase of outer mitochondrial membrane 20; TUBB: tubulin beta class I; WT: wild-type.


Subject(s)
Brain Ischemia , Mitophagy , Animals , Mice , Mitophagy/genetics , Sequestosome-1 Protein/metabolism , Infarction, Middle Cerebral Artery , Autophagy , Protein Kinases/metabolism , Fibroblasts/metabolism , Ubiquitination , Ubiquitin-Protein Ligases/metabolism , Membrane Proteins/metabolism , Mitochondrial Proteins/metabolism
2.
Mol Psychiatry ; 27(4): 2030-2041, 2022 04.
Article in English | MEDLINE | ID: mdl-35165395

ABSTRACT

Cerebellar deficits with Purkinje cell (PCs) loss are observed in several neurologic disorders. However, the underlying mechanisms as to how the cerebellum is affected during development remain unclear. Here we demonstrated that specific inactivation of murine Ebp1 in the central nervous system causes a profound neuropathology characterized by reduced cerebellar volume and PCs loss with abnormal dendritic development, leading to phenotypes including motor defects and schizophrenia (SZ)-like behaviors. Loss of Ebp1 leads to untimely gene expression of Fbxw7, an E3 ubiquitin ligase, resulting in aberrant protein degradation of PTF1A, thereby eliciting cerebellar defects. Reinstatement of Ebp1, but not the Ebp1-E183Ter mutant found in SZ patients, reconstituted cerebellar architecture with increased PCs numbers and improved behavioral phenotypes. Thus, our findings indicate a crucial role for EBP1 in cerebellar development, and define a molecular basis for the cerebellar contribution to neurologic disorders such as SZ.


Subject(s)
Cerebellar Diseases , DNA-Binding Proteins/metabolism , RNA-Binding Proteins/metabolism , Schizophrenia , Animals , Cerebellar Diseases/metabolism , Cerebellum/pathology , Humans , Mice , Purkinje Cells/metabolism , RNA-Binding Proteins/genetics , Schizophrenia/metabolism
3.
BMB Rep ; 54(8): 413-418, 2021 Aug.
Article in English | MEDLINE | ID: mdl-33691908

ABSTRACT

ErbB3-binding protein 1 (EBP1) is a multifunctional protein associated with neural development. Loss of Ebp1 leads to upregulation of the gene silencing unit suppressor of variegation 3-9 homolog 1 (Suv39H1)/DNA (cytosine 5)-methyltransferase (DNMT1). EBP1 directly binds to the promoter region of DNMT1, repressing DNA methylation, and hence, promoting neural development. In the current study, we showed that EBP1 suppresses histone methyltransferase activity of Suv39H1 by promoting ubiquitin-proteasome system (UPS)-dependent degradation of Suv39H1. In addition, we showed that EBP1 directly interacts with Suv39H1, and this interaction is required for recruiting the E3 ligase MDM2 for Suv39H1 degradation. Thus, our findings suggest that EBP1 regulates UPS-dependent degradation of Suv39H1 to govern proper heterochromatin assembly during neural development. [BMB Reports 2021; 54(8): 413-418].


Subject(s)
Adaptor Proteins, Signal Transducing/metabolism , DNA (Cytosine-5-)-Methyltransferase 1/metabolism , Methyltransferases/metabolism , RNA-Binding Proteins/metabolism , Repressor Proteins/metabolism , Adaptor Proteins, Signal Transducing/physiology , Animals , DNA Methylation , Gene Silencing , HEK293 Cells , Histone Methyltransferases/metabolism , Histones/metabolism , Humans , Male , Methyltransferases/physiology , Mice , Mice, Inbred C57BL , Mice, Knockout , Neurogenesis/genetics , Neurons/metabolism , Promoter Regions, Genetic/genetics , Proteasome Endopeptidase Complex/metabolism , Proteolysis , Proto-Oncogene Proteins c-mdm2/metabolism , RNA-Binding Proteins/physiology , Repressor Proteins/physiology , Ubiquitin/metabolism
4.
Sci Rep ; 8(1): 2557, 2018 02 07.
Article in English | MEDLINE | ID: mdl-29416050

ABSTRACT

Neurite growth is controlled by a complex molecular signaling network that regulates filamentous actin (F-actin) dynamics at the growth cone. The evolutionarily conserved ezrin, radixin, and moesin family of proteins tether F-actin to the cell membrane when phosphorylated at a conserved threonine residue and modulate neurite outgrowth. Here we show that Akt binds to and phosphorylates a threonine 573 residue on radixin. Akt-mediated phosphorylation protects radixin from ubiquitin-dependent proteasomal degradation, thereby enhancing radixin protein stability, which permits proper neurite outgrowth and growth cone formation. Conversely, the inhibition of Akt kinase or disruption of Akt-dependent phosphorylation reduces the binding affinity of radixin to F-actin as well as lowers radixin protein levels, resulting in decreased neurite outgrowth and growth cone formation. Our findings suggest that Akt signaling regulates neurite outgrowth by stabilizing radixin interactions with F-actin, thus facilitating local F-actin dynamics.


Subject(s)
Cytoskeletal Proteins/metabolism , Membrane Proteins/metabolism , Neuronal Outgrowth/physiology , Proteasome Endopeptidase Complex/metabolism , Proteolysis , Proto-Oncogene Proteins c-akt/metabolism , Actins/metabolism , Animals , Growth Cones/physiology , HEK293 Cells , Humans , Mice , Neurogenesis , Neuronal Outgrowth/genetics , PC12 Cells , Phosphorylation , Protein Binding , Protein Stability , Proto-Oncogene Proteins c-akt/genetics , Rats , Signal Transduction
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