Electrochemical recovery of nutrients from human urine coupled with biogas upgrading.

This study explored the coupling of electrochemical nutrient recovery from human urine with biogas upgrading. Ammonia nitrogen-rich (≥300 mM) and alkaline (≥pH 9) hydrolyzed urine (HU) is a promising alternative CO2 solvent. Spent urine after biogas upgrading (SU), with neutralized pH and increased conductivity resulting from CO2 absorption, is advantageous over HU for recovering total ammonia nitrogen (TAN) through electro-concentration. Experiments using synthetic urine at varying applied current densities (13-77 A/m2) demonstrated effective TAN recovery from both HU and SU, with greater enrichment factors at higher currents (2.1-3.3-fold, 1.2-1.8 M TAN concentrate). Validation experiments using real urine at the optimized current density of 52 A/m2, considering energy consumption, exhibited superior TAN recovery and energy efficiency when using SU (3.7-fold enrichment, 1.6 M TAN concentrate; suitable for liquid fertilizer) compared to HU. These findings offer an advanced strategy for maximizing urine valorization, contributing to a circular economy.

Protective Role of the Toll-Like Receptor 5 Agonist KMRC011 against Murine Colitis Induced by Citrobacter rodentium and Dextran Sulfate Sodium.

This study aimed to identify the therapeutic ability of a novel toll-like receptor (TLR) 5 agonist, KMRC011, on ulcerative colitis induced by Citrobacter rodentium and dextran sulfate sodium in a C57BL/6N mouse model. Ulcerative colitis was induced in the mice by the oral administration of 1% dextran sulfate sodium in sterile drinking water for seven days ad libitum, followed by C. rodentium infection on the seventh day by intra-gastric administration (DSS-CT group). KMRC011 was administered intramuscularly at both 24 h and 15 min before (Treatment 1 group), and at both 15 min and 24 h after (Treatment 2 group) the C. rodentium infection. The length of the large intestine and histopathological counts were significantly greater and mucosal thickness was significantly thinner in the Treatment 1 group compared to the DSS-CT and Treatment 2 groups. Il-6 and Il-10 mRNA expression levels were upregulated, while Ifn-γ and Tnf-α mRNA expression levels were significantly downregulated in the Treatment 1 group, compared to the DSS-CT group. NF-κB p65 expression level was elevated due to ulcerative colitis in the DSS-CT group, but was significantly downregulated in the Treatment 1 group. Overall, KMRC011 showed protective effects against murine colitis by inhibiting NF-κB signaling.

Potential treatment of aged cow manure using spare capacity in anaerobic digesters treating a mixture of food waste and pig manure.

With the increasing production of cow manure (CM) and the continuing decrease in the demand for manure compost, CM management has become an urgent and challenging task in Korea. In most cattle farms in Korea, CM mixed with bedding materials is left in pens exposed to the open air for several months before treatment, which makes CM an unsuitable feedstock for anaerobic digestion. This study examined the co-digestion of aged CM with a mixture of food waste and pig manure as the base substrate to assess the possibility of treating and valorizing CM using spare capacity in existing anaerobic digesters dealing with other wastes. The duplicate digesters initially fed with the base substrate were subjected to the addition of increasing amounts of CM (3-10% in the feed, w/v) over nine months. Co-feeding CM up to 5% in the feed (w/v) did not compromise the methanogenic degradation of the substrates, but adding more CM led to a significant performance deterioration likely related to the buildup of inhibitory free ammonia and H2S. Adding CM substantially influenced the digester microbial communities, especially methanogenic communities, and induced a dominance shift from aceticlastic Methanothrix to hydrogenotrophic methanogens as the CM fraction increased. The overall results suggest that the CM fraction should not exceed 5% in the feed (w/v) for its stable treatment with the base substrate in the experimental digesters. Although further studies are needed, anaerobic treatment using spare capacity in existing digesters can be a useful strategy for the management of aged CM.

Rag2 Deficiency Enhances Susceptibility to Systemic Mouse Adenovirus Type 1 Infection.

INTRODUCTION: Recombination-activating gene (Rag) 1 and Rag2, which are essential in V(D)J recombination, play a crucial role in B- and T-cell maturation. METHOD: We investigated the effects of Rag2 deficiency in clustered regularly interspaced short palindromic repeats/Cas9-mediated FVB-Rag2 knockout (KO) and wild-type (WT) mice infected with mouse adenovirus type 1 (MAV-1) via the intranasal route. RESULTS: MAV-1 infection caused more severe histopathological changes in FVB-Rag2 KO mice than in WT mice. FVB-Rag2 KO mice exhibited moderate to severe inflammation on day 4 and severe inflammation on day 8 post infection. In contrast, WT mice showed mild inflammation on day 4 and mild to severe inflammation on day 8 post infection, including interstitial pneumonia and inflammatory cell infiltration in the lungs and liver. Viral loads in the spleen and kidneys were significantly higher in FVB-Rag2 KO mice than in WT mice on day 8 post infection. Levels of cytokines and chemokines, including macrophage inflammatory protein-1α, induced protein 10, interferon (IFN)-α, IFN-γ, and tumor necrosis factor alpha, were upregulated in the spleens of FVB-Rag2 KO mice compared with those of WT mice. The upregulation of several cytokines occurred concurrently with the histopathological changes. MAV-1 infection induced more severe systemic infection in FVB-Rag2 KO mice than in WT mice. CONCLUSION: In mice, Rag2 deficiency induces inflammatory cell recruitment via the upregulation of cytokine and chemokine levels. The MAV-1 infection model can be utilized to assess the efficacy and safety of therapeutic agents for human adenoviral diseases.

The impact of the abnormal salinity enrichment in pore water on the thermodynamic stability of marine natural gas hydrates in the Arctic region.

In this study, the thermodynamic and structural characteristics of natural gas hydrates (NGHs) retrieved from gas hydrate mounds (ARAON Mound 03 (AM03) and ARAON Mound 06 (AM06)) in the Chukchi Sea in the Arctic region were investigated. The gas compositions, crystalline structure, and cage occupancy of the NGHs at AM03 and AM06 were experimentally measured using gas chromatography (GC), 13C nuclear magnetic resonance (NMR), Raman spectroscopy, and powder X-ray diffraction (PXRD). In the NGHs from AM03 and AM06, a significantly large fraction of CH4 (> 99%) and a very small amount of H2S were enclathrated in small (512) and large (51262) cages of sI hydrate. The NGHs from AM03 and AM06 were almost identical in composition, guest distributions, and existing environment to each other. The salinity of the residual pore water in the hydrate-bearing sediment (AM06) was measured to be 50.32‰, which was much higher than that of seawater (34.88‰). This abnormal salinity enrichment in the pore water of the low-permeability sediment might induce the dissociation of NGHs at a lower temperature than expected. The saturation changes in the NGHs that corresponded with an increase in the seawater temperature were also predicted on the basis of the salinity changes in the pore water. The experimental and predicted results of this study would be helpful for understanding the thermodynamic stability of NGHs and potential CH4-releasing phenomena in the Arctic region.

Long-term monitoring of a thermal hydrolysis-anaerobic co-digestion plant treating high-strength organic wastes: Process performance and microbial community dynamics.

Two parallel anaerobic digesters (8500 m3 capacity each), combined with thermal hydrolysis (TH) pretreatment, co-digesting dewatered sewage sludge, dewatered human feces, and food wastewater were monitored over a 12-month period from start-up to explore the feasibility of field application of the combined process. The waste mixtures before and after pretreatment and the feed and digestate of each digester were taken semimonthly (i.e., 48 samples in total) for analysis of the feed characteristics, process parameters, and digester microbial community structure. The TH pretreatment proved effective in improving the bioavailability of the waste mixture. The solubilization efficiency tended to increase with the particulate organic fraction in the raw mixture. Although fluctuations in the feed characteristics and loading significantly influenced the process and microbial behaviors, the digesters maintained stable performance during the study period. Our results demonstrate that the TH-anaerobic digestion process can achieve an effective and robust treatment of the waste mixture.

Treatment of Cattle Manure by Anaerobic Co-Digestion with Food Waste and Pig Manure: Methane Yield and Synergistic Effect.

The management of cattle manure (CM) has become increasingly challenging because its production continues to rise, while the regulations on manure management have become increasingly stringent. In Korea, most farms produce CM as a dry mixture with lignocellulosic bedding materials (mainly sawdust), making it impractical to treat CM by anaerobic digestion. To address this problem, this study examined whether anaerobic co-digestion with food waste (FW) and pig manure (PM) could be an effective approach for the treatment of CM. The batch anaerobic digestion tests at different CM: FW: PM mixing ratios showed that more methane was produced as the FW fraction increased, and as the CM fraction decreased. The response surface models describing how the substrate mixing ratio affects the methane yield and synergistic effect (methane yield basis) were successfully generated. The models proved that the methane yield and synergistic effect respond differently to changes in the substrate mixing ratio. The maximum 30-day methane yield was predicted at 100% FW, whereas the maximum 30-day synergy index was estimated for the mixture of 47% CM, 6% FW, and 47% PM (total solids basis). The synergy index model showed that CM, FW, and PM could be co-digested without a substantial loss of their methane potential at any mixing ratio (30-day synergy index, 0.89-1.22), and that a possible antagonistic effect could be avoided by keeping the FW proportion less than 50%. The results suggest that co-digestion with PM and FW could be flexibly applied for the treatment and valorization of CM in existing anaerobic digestion plants treating FW and PM.

Comparison of the virulence of Streptococcus pneumoniae in ICR mouse stocks of three different origins.

Streptococcus pneumoniae causes many people to suffer from pneumonia, septicemia, and other diseases worldwide. To identify the difference in susceptibility of and treatment efficacy against S. pneumoniae in three ICR mouse stocks (Korl:ICR, A:ICR, and B:ICR) with different origins, mice were infected with 2 × 106, 2 × 107, and 2 × 108 CFU of S. pneumoniae D39 intratracheally. The survival of mice was observed until three weeks after the infection. The three stocks of mice showed no significant survival rate difference at 2 × 106 and 2 × 107 CFU. However, the lung and spleen weight in the A:ICR stock was significantly different from that in the other two stocks, whereas the liver weight in B:ICR stock was significantly lower than that in the other two stocks. Interestingly, no significant CFU difference in the organs was observed between the ICR stocks. The level of interferon gamma inducible protein 10 in Korl:ICR was significantly lower than that in the other two stocks. The level of granulocyte colony stimulating factor in B:ICR was significantly lower than in the other two stocks. However, tumor-necrosis factor-alpha and interleukin-6 levels showed no significant difference between the ICR stocks. In the vancomycin efficacy test after the S. pneumoniae infection, both the single-dose and double-dose vancomycin-treated groups showed a significantly better survival rate than the control group. There was no significant survival difference between the three stocks. These data showed that Korl:ICR, A:ICR, and B:ICR have no susceptibility difference to the S. pneumoniae D39 serotype 2.