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J Cell Physiol ; 234(11): 20888-20899, 2019 11.
Article in English | MEDLINE | ID: mdl-30997682

ABSTRACT

It has been reported that asprosin is a novel adipokine which is augmented in mice and humans with type 2 diabetes (T2DM). Asprosin stimulates hepatic gluconeogenesis under fasting conditions. However, the roles of asprosin in inflammation, endoplasmic reticulum (ER) stress, and insulin resistance in skeletal muscle has not been studied. In the currents study, elevated levels of asprosin expression were observed in adipocytes under hyperlipidemic conditions. Treatment of C2C12 myocytes with asprosin-induced ER stress markers (phosphorylated inositol-requiring enzyme 1 and eukaryotic initiation factor 2, and CHOP expression) as well as inflammation markers (interleukin-6 expression, phosphorylated IκB, and nuclear translocated nuclear factor-κß). Finally, asprosin treatment promoted exacerbation of insulin sensitivity as determined by levels of insulin receptor substrate 1 and Akt phosphorylation as well as glucose uptake. Moreover, treatment of asprosin augmented protein kinase C-δ (PKCδ) phosphorylation and nuclear translocation, but suppressed messenger RNA expression of sarcoplasmic reticulum Ca2+ ATPase 2b in both C2C12 myocytes and in mouse soleus skeletal muscle. These asprosin-induced effects were markedly decreased in small interfering (si) RNA-mediated PKCδ-knockdown in C2C12 myocytes. These results suggest that asprosin results in impairment of insulin sensitivity in skeletal muscle through PKCδ-associated ER stress/inflammation pathways and may be a valuable strategy for management of insulin resistance and T2DM.


Subject(s)
Endoplasmic Reticulum Stress , Fibrillin-1/metabolism , Inflammation/pathology , Insulin/metabolism , Muscle, Skeletal/pathology , Peptide Fragments/metabolism , Peptide Hormones/metabolism , Protein Kinase C-delta/metabolism , Signal Transduction , 3T3-L1 Cells , Animals , Disease Models, Animal , Glucose/metabolism , Hyperlipidemias/complications , Hyperlipidemias/pathology , Inflammation/complications , Insulin Resistance , Male , Mice , Mice, Inbred C57BL , Muscle Cells/metabolism , Muscle Cells/pathology , Muscle, Skeletal/enzymology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Reactive Oxygen Species/metabolism , Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism
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