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1.
Small ; 17(50): e2102618, 2021 Dec.
Article in English | MEDLINE | ID: mdl-34655151

ABSTRACT

Metal foil electrodes are simple to prepare and have a high active material loading, making them well suited for the fabrication of inexpensive high-energy-density batteries. Herein, Sn metal foil is used as a binder- and conductive additive-free anode for sodium-ion batteries, achieving a high reversible specific capacity of 692 mAh g-1 and coulombic efficiency of 99% after 100 cycles at a rate of 0.1 C. During the first discharge process, the anode undergoes area expansion. It then splits into multiple parts during the first-charge process. Upon cycling, the separated parts reconnect and form a single piece with a porous and robust coral structure owing to the self-healing nature of the anode. A full cell with a Sn foil anode and Na3 V2 (PO4 )3 cathode shows a stable cycle life of 100 mAh g-1 for 300 cycles. Thus, the cracking or pulverization of the Sn anode is not the principal origin of poor cycling properties. The adopted strategy will promote the development and commercialization of high-capacity metal foil anodes that undergo volume changes during charge/discharge cycling.

2.
Nanoscale ; 13(23): 10447-10454, 2021 Jun 17.
Article in English | MEDLINE | ID: mdl-34076016

ABSTRACT

Transition-metal sulfides have been extensively studied as anode materials for use in sodium-ion batteries (SIBs) and potassium-ion batteries (PIBs) due to their multi-electron reactions, high rate performance, and abundant available resources. However, the practical capacities of metal sulfides remain low due to conductivity issues, volume expansion, and the use of traditional carbonate electrolytes. To overcome these drawbacks, ether electrolytes can be combined with nanoparticle-based metal sulfide anodes. Herein, a nanoparticle-based nickel monosulfide (NiS) anode with high rate performance in the ether electrolytes of SIBs/PIBs was prepared by heating a mixture of nickel nanoparticles with sulfur. In SIBs, the NiS anode capacity was 286 mA h g-1 at a high current density of 100 A g-1, and excellent cycling performance was observed at 25 A g-1 with a capacity of 468 mA h g-1 after 1000 cycles. Moreover, a full-cell containing a Na3V2(PO4) cathode demonstrated a rate performance of 65 mA h g-1 at a high current density of 100 A g-1. In PIBs, the NiS electrode capacity was 642 and 37 mA h g-1 at 0.5 and 100 A g-1, respectively. Hence, the synthesised NiS nanoparticles possessed excellent storage capability, regardless of the alkali-ion type, suggesting their potential use as robust NiS anodes for advanced battery systems.

3.
ChemSusChem ; 14(8): 1936-1947, 2021 Apr 22.
Article in English | MEDLINE | ID: mdl-33638280

ABSTRACT

Iron sulfide (FeS) anodes are plagued by severe irreversibility and volume changes that limit cycle performances. Here, a synergistically coupled hybrid composite, nanoengineered iron sulfide/S-doped graphene aerogel, was developed as high-capacity anode material for Li/Na-ion half/full batteries. The rational coupling of in situ generated FeS nanocrystals and the S-doped rGO aerogel matrix boosted the electronic conductivity, Li+ /Na+ diffusion kinetics, and accommodated the volume changes in FeS. This anode system exhibited excellent long-term cyclability retaining high reversible capacities of 422 (1100 cycles) and 382 mAh g-1 (1600 cycles), respectively, for Li+ and Na+ storage at 5 A g-1 . Full batteries designed with this anode system exhibited 435 (FeS/srGOA||LiCoO2 ) and 455 mAh g-1 (FeS/srGOA||Na0.64 Co0.1 Mn0.9 O2 ). The proposed low-cost anode system is competent with the current Li-ion battery technology and extends its utility for Na+ storage.

4.
RSC Adv ; 11(26): 16122-16130, 2021 Apr 26.
Article in English | MEDLINE | ID: mdl-35481196

ABSTRACT

Sulfurized polyacrylonitrile (SPAN) is a promising active material for Li/S batteries owing to its high sulfur utilization and long-term cyclability. However, because SPAN electrodes are synthesized using powder, they require large amounts of electrolyte, conducting agents, and binder, which reduces the practical energy density. Herein, to improve the practical energy density, we fabricated bulk-type SPAN disk cathodes from pressed sulfur and polyacrylonitrile powders using a simple heating process. The SPAN disks could be used directly as cathode materials because their π-π structures provide molecular-level electrical connectivity. In addition, the electrodes had interconnected pores, which improved the mobility of Li+ ions by allowing homogeneous adsorption of the electrolyte. The specific capacity of the optimal electrode was very high (517 mA h gelectrode -1). Furthermore, considering the weights of the anode, separator, cathode, and electrolyte, the Li/S cell exhibited a high practical energy density of 250 W h kg-1. The areal capacity was also high (8.5 mA h cm-2) owing to the high SPAN loading of 16.37 mg cm-2. After the introduction of 10 wt% multi-walled carbon nanotubes as a conducting agent, the SPAN disk electrode exhibited excellent cyclability while maintaining a high energy density. This strategy offers a potential candidate for Li/S batteries with high practical energy densities.

5.
J Nanosci Nanotechnol ; 20(11): 7092-7095, 2020 Nov 01.
Article in English | MEDLINE | ID: mdl-32604563

ABSTRACT

A polyacrylonitrile (PAN) nanofiber web was prepared by electrospinning PAN solutions with dimethyl sulfoxide (DMSO) or Dimethylformamide (DMF) as solvent. The PAN web was heated with sulfur to synthesize a sulfurized polyacrylonitrile (SPAN) nanofiber web. The shape of the SPAN web was found to depend on concentration of the PAN solution and properties of the solvent. The SPAN web synthesized using 8 wt% PAN solution in DMSO showed the highest capacity of 1053.3 mAh g-1sulfur under a current density of 526 mA g-1sulfur. Thus, we determined that DMSO could be a potential solvent for preparation of SPAN web electrodes.

6.
Nanoscale Adv ; 2(11): 5166-5170, 2020 Nov 11.
Article in English | MEDLINE | ID: mdl-36132030

ABSTRACT

Sodium-ion batteries (SIBs) are a viable substitute for lithium-ion batteries due to the low cost and wide availability of sodium. However, practical applications require the development of fast charging sodium-ion-based full-cells with high power densities. Na3V2(PO4)3 (NVP) is a bipolar material with excellent characteristics as both a cathode and an anode material in SIBs. Designing symmetric cells with NVP results in a single voltage plateau with significant specific capacity which is ideal for a full cell. Here we demonstrate for the first time a tremendous improvement in the performance of NVP symmetric full cells by introducing an ether-based electrolyte which favors fast reaction kinetics. In a symmetric full cell configuration, 75.5% of the initial capacity was retained even after 4000 cycles at 2 A g-1, revealing ultra-long cyclability. Excellent rate performances were obtained at current densities as high as 1000C, based on the cathode mass, revealing ultrafast Na+ transfer. The power density obtained for this NVP symmetric cell (48 250 W kg-1) is the best among those of all the sodium-ion-based full cells reported to date.

7.
J Nanosci Nanotechnol ; 18(9): 6422-6426, 2018 09 01.
Article in English | MEDLINE | ID: mdl-29677807

ABSTRACT

Tin monoxide (SnO) anodes are promising candidates for use in sodium-ion batteries because of their high theoretical capacities and stable cycle performance. In previous reports, electrodes with excellent performance have been prepared by using nano-sized SnO particles. However, the synthesis of nano-sized SnO particles is complex, time-consuming, and expensive. In this paper, an anode of micron-sized SnO is prepared by using commercial micron-sized SnO particles. The electrode exhibits a reversible capacity of 450 mAh g-1 in the 1st cycle at a current rate of 100 mA g-1. We used a tetraethylene glycol dimethyl ether (TEGDME)-based electrolyte, which is well known for its superior electrochemical performance in sodium-ion batteries. The mechanism of operation of the anode containing micron-sized SnO particles has been confirmed by a detailed study using X-ray diffraction (XRD), scanning electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS). During initial discharge, the SnO changed to Sn and sodium oxide, and the surface of the electrode became covered with a film. The electrode composed of micron-sized SnO is a potential candidate for use in sodium-ion batteries.

8.
J Nanosci Nanotechnol ; 18(9): 6524-6527, 2018 09 01.
Article in English | MEDLINE | ID: mdl-29677826

ABSTRACT

A 3D sulfur cathode for a large-scale room-temperature (RT) Na/S battery with a high sulfur loading of 14.63 mg cm-2 was fabricated. The first discharge was tested in order to understand the macroscopic changes. A first discharge capacity of 865 mAh g-1 was obtained at 1/1000 C-rate along with a discharge curve with two clear plateaus at 2.29 V and 1.78 V, respectively. A visual change occurs in the 3D sulfur cathode. A thick layer of discharge products at the solid electrolyte separator face of the 3D sulfur cathode was observed and analyzed using a scanning electron microscope (SEM) coupled with energy dispersive X-ray spectroscopy (EDS).

9.
Toxicol Appl Pharmacol ; 287(2): 119-127, 2015 Sep 01.
Article in English | MEDLINE | ID: mdl-25981167

ABSTRACT

As the importance of allergic disorders such as atopic dermatitis and allergic asthma, research on potential drug candidates becomes more necessary. Mast cells play an important role as initiators of allergic responses through the release of histamine; therefore, they should be the target of pharmaceutical development for the management of allergic inflammation. In our previous study, anti-allergic effect of extracts of Amomum xanthioides was demonstrated. To further investigate improved candidates, 1,2,4,5-tetramethoxybenzene (TMB) was isolated from methanol extracts of A. xanthioides. TMB dose-dependently attenuated the degranulation of mast cells without cytotoxicity by inhibiting calcium influx. TMB decreased the expression of pro-inflammatory cytokines such as tumor necrosis factor-α and interleukin (IL)-4 at both the transcriptional and translational levels. Increased expression of these cytokines was caused by translocation of nuclear factor-κB into the nucleus, and it was hindered by suppressing activation of IκB kinase complex. To confirm the effect of TMB in vivo, the ovalbumin (OVA)-induced active systemic anaphylaxis (ASA) and IgE-mediated passive cutaneous anaphylaxis (PCA) models were used. In the ASA model, hypothermia was decreased by oral administration of TMB, which attenuated serum histamine, OVA-specific IgE, and IL-4 levels. Increased pigmentation of Evans blue was reduced by TMB in a dose-dependent manner in the PCA model. Our results suggest that TMB is a possible therapeutic candidate for allergic inflammatory diseases that acts through the inhibition of mast cell degranulation and expression of pro-inflammatory cytokines.


Subject(s)
Anisoles/pharmacology , Inflammation/drug therapy , Inflammation/physiopathology , Amomum , Animals , Cell Degranulation/drug effects , Cytokines/antagonists & inhibitors , Dose-Response Relationship, Drug , Hypersensitivity , I-kappa B Kinase/biosynthesis , Inflammation Mediators/antagonists & inhibitors , Male , Mast Cells/drug effects , Mice , Plant Extracts/pharmacology , Rats , Rats, Sprague-Dawley
10.
Exp Biol Med (Maywood) ; 240(5): 631-8, 2015 May.
Article in English | MEDLINE | ID: mdl-25349218

ABSTRACT

In this study, we investigated the effect of 3,4,5-trihydroxy-N-(8-hydroxyquinolin-2-yl)benzamide) (SG-HQ2), a synthetic analogue of gallic acid (3,4,5-trihydroxybenzoic acid), on the mast cell-mediated allergic inflammation and the possible mechanism of action. Mast cells play major roles in immunoglobulin E-mediated allergic responses by the release of histamine, lipid-derived mediators, and pro-inflammatory cytokines. We previously reported the potential effects of gallic acid using allergic inflammation models. For incremental research, we synthesized the SG-HQ2 by the modification of functional groups from gallic acid. SG-HQ2 attenuated histamine release by the reduction of intracellular calcium in human mast cells and primary peritoneal mast cells. The inhibitory efficacy of SG-HQ2 was similar with gallic acid. Enhanced expression of pro-inflammatory cytokines such as tumor necrosis factor-α, interleukin-1ß, interleukin-4, and interleukin-6 in activated mast cells was significantly diminished by SG-HQ2 100 times lower concentration of gallic acid. This inhibitory effect was mediated by the reduction of nuclear factor-κB. In animal models, SG-HQ2 inhibited compound 48/80-induced serum histamine release and immunoglobulin E-mediated local allergic reaction, passive cutaneous anaphylaxis. Our results indicate that SG-HQ2, an analogue of gallic acid, might be a possible therapeutic candidate for mast cell-mediated allergic inflammatory diseases through suppression of histamine release and pro-inflammatory cytokines.


Subject(s)
Cytokines/metabolism , Gallic Acid/analogs & derivatives , Histamine Release/drug effects , Hydroxyquinolines/pharmacology , Hypersensitivity/prevention & control , Inflammation Mediators/metabolism , Inflammation/prevention & control , Mast Cells/drug effects , Animals , Calcium/metabolism , Gallic Acid/pharmacology , Immunoglobulin E/administration & dosage , Male , Mast Cells/metabolism , Mice , NF-kappa B/metabolism , Passive Cutaneous Anaphylaxis , Rats , Rats, Sprague-Dawley , Real-Time Polymerase Chain Reaction
11.
Arch Pharm Res ; 37(4): 467-72, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24014307

ABSTRACT

A new neolignan, linderin A (1), together with six known lignans, (+)-xanthoxyol (2), pluviatilol (3), actiforin (4), (+)-syringaresinol (5), (+)-(7S,8R,8'R)-acuminatolide (6), and (+)-9'-O-trans-feruloyl-5,5'-dimethoxylariciresinol (7) were isolated from the stems of Lindera obtusiloba Blume (Lauraceae). Their chemical structures were elucidated by a combination of spectroscopic analysis and chemical reaction, and the absolute configuration of 1 was determined by Mosher's esterification. The effect of compounds 1-7 on tumor necrosis factor (TNF)-α, interleukin(IL)-6, and their inhibitory activity of histamine release were examined using human mast cells. Among the lignans tested, compounds 1, 3, 4, 6, and 7 inhibited release of histamine from mast cells. Especially, compounds 1 and 4 suppressed the gene expressions of pro-inflammatory cytokines, TNF-α, and IL-6 in human mast cells. Our findings suggest that the lignan constituents in L. obtusiloba may contribute to its anti-allergic inflammatory effects.


Subject(s)
Anti-Allergic Agents/isolation & purification , Anti-Allergic Agents/pharmacology , Lignans/isolation & purification , Lignans/pharmacology , Lindera/chemistry , Anti-Allergic Agents/chemistry , Histamine Release/drug effects , Humans , Interleukin-6/metabolism , Lignans/chemistry , Mast Cells/drug effects , Mast Cells/metabolism , Plant Extracts/pharmacology , Plant Stems/chemistry , Structure-Activity Relationship , Tumor Necrosis Factor-alpha/metabolism
12.
Toxicol Appl Pharmacol ; 274(3): 455-61, 2014 Feb 01.
Article in English | MEDLINE | ID: mdl-24361550

ABSTRACT

A great number of people are suffering from allergic inflammatory disease such as asthma, atopic dermatitis, and sinusitis. Therefore discovery of drugs for the treatment of these diseases is an important subject in human health. Putranjivain A (PJA), member of ellagitannin, is known to possess beneficial effects including anti-cancer and anti-viral activities. The aim of the present study was to elucidate whether PJA modulates the allergic inflammatory reaction and to study its possible mechanisms of action using mast cell-based in vitro and in vivo models. The study was performed in anaphylaxis mouse model and cultured mast cells. PJA inhibited the expression of pro-inflammatory cytokines in immunoglobulin E-stimulated mast cells. PJA reduced this expression by inhibiting nuclear factor (NF)-κB and nuclear factor of activated T cell. The oral administration of PJA reduced systemic and cutaneous anaphylaxis, the release of serum histamine, and the expression of the histamine H1 receptor. In addition, PJA attenuated the activation of mast cells. PJA inhibited the release of histamine from various types of mast cells by the suppression of intracellular calcium. The inhibitory activity of PJA on the allergic reaction was similar to that of disodium cromoglycate, a known anti-allergic drug. These results suggest that PJA can facilitate the prevention or treatment of allergic inflammatory diseases mediated by mast cells.


Subject(s)
Gallic Acid/analogs & derivatives , Glucosides/pharmacology , Inflammation/prevention & control , Mast Cells/drug effects , Administration, Oral , Animals , Anti-Asthmatic Agents/pharmacology , Cells, Cultured , Cromolyn Sodium/pharmacology , Cytokines/antagonists & inhibitors , Cytokines/metabolism , Gallic Acid/pharmacology , Histamine Release/drug effects , Humans , Hypersensitivity/drug therapy , Immunoglobulin E/metabolism , Inflammation/drug therapy , Male , Mast Cells/metabolism , Mice , Mice, Inbred ICR , NF-kappa B/antagonists & inhibitors , NF-kappa B/metabolism , Rats , Receptors, Histamine H1/genetics , Receptors, Histamine H1/metabolism
13.
Int J Mol Med ; 32(4): 945-51, 2013 Oct.
Article in English | MEDLINE | ID: mdl-23921373

ABSTRACT

Diospyros kaki (D. kaki) has been cultivated throughout Eastern Asia for hundreds of years. D. kaki contains various biological active compounds, such as amino acids, carotenoids, flavonoids, tannins, catechins and vitamin A. Previous studies have shown that D. kaki has beneficial effects on homeostasis, constipation, hypertension, atherosclerosis and allergic dermatitis and is a good source of antioxidants, polyphenols and dietary fiber. However, the anti-allergic and anti-inflammatory effects of D. kaki have not yet been elucidated. This study aimed to investigate the protective effects of the aqueous extract of Diospyros kaki (AEDK) on mast cell-mediated allergic inflammation and to determine its possible mechanisms of action by using in vitro and in vivo mast cell-based models. The cAMP and intracellular calcium levels were measured to clarify the mechanisms by which AEDK inhibits the release of histamine from mast cells. AEDK inhibited the release of histamine and ß-hexosaminidase from mast cells by modulating cAMP and intracellular calcium levels. We also measured the expression of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-1ß. AEDK decreased gene expression and the secretion of the pro-inflammatory cytokines, TNF-α and IL-1ß by inhibiting nuclear factor-κB. In addition, AEDK inhibited systemic and cutaneous allergic reaction. The inhibitory effects of AEDK on allergic reaction and the release of histamine were found to be similar to those of disodium cromoglycate, a known anti-allergic drug. To isolate the active component of AEDK, activity-guided fractionation was performed, based on the inhibitory effects on systemic anaphylaxis. Catechin was identified as an active compound. The present findings provide evidence that AEDK inhibits allergic inflammation and suggest the therapeutic application of AEDK in allergic inflammatory disorders.


Subject(s)
Calcium/metabolism , Diospyros/chemistry , Inflammation/drug therapy , Plant Extracts/pharmacology , Animals , Anti-Asthmatic Agents/pharmacology , Cell Line , Cromolyn Sodium/pharmacology , Disease Models, Animal , Histamine Release/drug effects , Humans , Hypersensitivity/drug therapy , Interleukin-1beta/genetics , Interleukin-1beta/metabolism , Interleukin-6/genetics , Interleukin-6/metabolism , Male , Mast Cells/drug effects , Mast Cells/metabolism , Mice , Mice, Inbred ICR , NF-kappa B/antagonists & inhibitors , NF-kappa B/genetics , NF-kappa B/metabolism , Tumor Necrosis Factor-alpha/genetics , Tumor Necrosis Factor-alpha/metabolism
14.
Int J Mol Med ; 32(3): 736-42, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23828310

ABSTRACT

The aim of the present study was to elucidate whether extracts of Vigna angularis (EVA) inhibit allergic inflammatory reactions and to elucidate the possible mechanisms of action. For the assessment of allergic inflammatory response, histamine release and the expression of pro-inflammatory cytokines from human mast cells (HMC-1) were examined. To identify the underlying mechanisms of action, intracellular calcium and the activation of nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) were assayed. To confirm the effects of EVA in vivo, systemic and local allergic reaction mouse models were employed. EVA dose-dependently reduced phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-induced histamine release from mast cells. The inhibitory effects of EVA on the release of histamine from mast cells were mediated by the reduction of intracellular calcium levels. EVA decreased the PMACI-stimulated gene expression and secretion of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6. The inhibitory effects of EVA on pro-inflammatory cytokines were NF-κB- and MAPK-dependent. In addition, EVA inhibited compound 48/80-induced systemic anaphylaxis and immunoglobulin E (IgE)-mediated cutaneous anaphylaxis. Our findings provide evidence that EVA inhibits mast cell-derived allergic inflammation, and suggest the possible therapeutic application of EVA in allergic inflammatory disorders.


Subject(s)
Anti-Allergic Agents/pharmacology , Fabaceae/chemistry , Hypersensitivity/immunology , Mast Cells/drug effects , Mast Cells/immunology , Plant Extracts/pharmacology , Anaphylaxis/drug therapy , Anaphylaxis/immunology , Anaphylaxis/metabolism , Animals , Anti-Allergic Agents/administration & dosage , Calcium/metabolism , Cell Line , Cytokines/biosynthesis , Disease Models, Animal , Dose-Response Relationship, Drug , Histamine Release/drug effects , Histamine Release/immunology , Humans , Hypersensitivity/drug therapy , Hypersensitivity/metabolism , Inflammation Mediators/metabolism , Male , Mast Cells/metabolism , Mice , Mitogen-Activated Protein Kinases/metabolism , NF-kappa B/metabolism , Plant Extracts/administration & dosage , Plant Extracts/genetics , Signal Transduction/drug effects
15.
Food Chem Toxicol ; 57: 209-16, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23535185

ABSTRACT

A great number of people are suffering from allergic inflammatory disease such as asthma, atopic dermatitis, and sinusitis. Therefore discovery of drugs for the treatment of these diseases is an important subject in human health. In this study, we investigated anti-allergic inflammatory effect of galangin and underlying mechanisms of action using in vitro and in vivo models. Galangin inhibited histamine release by the reduction of intracellular calcium in phorbol 12-mystate 13-acetate plus calcium ionophore A23187-stimulated human mast cells (HMC-1). Galangin decreased expression of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1ß, and IL-8. The inhibitory effect of galangin on theses pro-inflammatory cytokines was related with c-Jun N-terminal kinases, and p38 mitogen-activated protein kinase, nuclear factor-κB, and caspase-1. Furthermore, galangin attenuated IgE-mediated passive cutaneous anaphylaxis and the expression of histamine receptor 1 at the inflamed tissue. The inhibitory effects of galangin were more potent than cromolyn, a known anti-allergic drug. Our results showed that galangin down-regulates mast cell-derived allergic inflammatory reactions by blocking histamine release and expression of pro-inflammatory cytokines. In light of in vitro and in vivo anti-allergic inflammatory effects, galangin could be a beneficial anti-allergic inflammatory agent.


Subject(s)
Flavonoids/pharmacology , Hypersensitivity/drug therapy , Inflammation/drug therapy , Mast Cells/drug effects , Anaphylaxis/drug therapy , Anaphylaxis/immunology , Animals , Anti-Allergic Agents/pharmacology , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Calcium/metabolism , Caspase 1/metabolism , Cells, Cultured , Cytokines/metabolism , Histamine Release/drug effects , Humans , Hypersensitivity/metabolism , Inflammation/metabolism , Inflammation/pathology , Male , Mast Cells/immunology , Mast Cells/metabolism , Mice, Inbred ICR , NF-kappa B/antagonists & inhibitors , NF-kappa B/metabolism , p38 Mitogen-Activated Protein Kinases/metabolism
16.
Int J Mol Med ; 31(4): 951-8, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23426871

ABSTRACT

Piceatannol is a phenolic stilbenoid and a metabolite of resveratrol which is found in red wine. Piceatannol (PIC) commonly exhibits anti-inflammatory, antiplatelet and antiproliferative activity. In the present study, the anti-allergic and anti-inflammatory mechanisms of PIC were investigated by examining the effects of PIC on pro­inflammatory cytokine release and phosphorylation of mitogen-activated protein (MAP) kinases (ERK, JNK and p38) in a human mast cell line. PIC dose-dependently inhibited compound 48/80-induced systemic anaphylaxis and immunoglobulin E-mediated local allergic reactions. PIC reduced the immunoglobulin E (IgE)-mediated local allergic reaction and attenuated histamine release from rat peritoneal mast cells. Histamine and ß-hexosaminidase release was markedly decreased dose-dependently by PIC treatment in RBL-2H3 cells. PIC treatments of HMC-1 cells definitely reduced mRNA expression and the release of the pro­inflammatory cytokines, tumor necrosis factor-α and interleukin-8. MAP kinase phosphorylation was also strongly decreased dose-dependently following PIC treatment. PIC regulated the production of cytokines and histamine in phorbol 12-myristate 13-acetate plus A23187-stimulated mast cells. Thus, PIC may alleviate allergic inflammation and may be a useful therapeutic agent for allergic diseases.


Subject(s)
Anti-Inflammatory Agents/pharmacology , Mast Cells , Signal Transduction/drug effects , Stilbenes/pharmacology , Anaphylaxis/chemically induced , Animals , Anti-Inflammatory Agents/chemistry , Cell Line , Cell Survival/drug effects , Cytokines/analysis , Cytokines/genetics , Cytokines/metabolism , Extracellular Signal-Regulated MAP Kinases/metabolism , Histamine/metabolism , Humans , Male , Mast Cells/drug effects , Mast Cells/metabolism , Mice , Mice, Inbred ICR , Stilbenes/chemistry , beta-N-Acetylhexosaminidases/metabolism , p-Methoxy-N-methylphenethylamine/toxicity
17.
Am J Chin Med ; 40(6): 1257-70, 2012.
Article in English | MEDLINE | ID: mdl-23227796

ABSTRACT

Allergic inflammatory diseases such as food allergy, asthma, sinusitis, and atopic dermatitis are increasing worldwide. In this study, we investigated the effects of aqueous extract of Mosla chinensis Max. (AMC) on mast cell-mediated allergic inflammation and studied the possible mechanism of this action. AMC inhibited compound 48/80-induced systemic and immunoglobulin E (IgE)-mediated local anaphylaxis. AMC reduced intracellular calcium levels and downstream histamine release from rat peritoneal mast cells activated by compound 48/80 or IgE. In addition, AMC decreased gene expression and secretion of proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 in human mast cells. The inhibitory effect of AMC on cytokine expression was nuclear factor (NF)-κB dependent. Our results indicate that AMC inhibits mast cell-mediated allergic inflammatory reaction by suppressing histamine release and expression of proinflammatory cytokines and the involvement of calcium and NF-κB in these effects. AMC might be a possible therapeutic candidate for allergic inflammatory disorders.


Subject(s)
Hypersensitivity/prevention & control , Inflammation/prevention & control , Lamiaceae/chemistry , Mast Cells/drug effects , Plant Extracts/pharmacology , Animals , Blotting, Western , Cytokines/metabolism , Enzyme-Linked Immunosorbent Assay , Histamine Release/drug effects , Hypersensitivity/immunology , Hypersensitivity/metabolism , Inflammation/immunology , Inflammation Mediators/metabolism , Male , Mast Cells/metabolism , Mice , Mice, Inbred ICR , Rats , Rats, Sprague-Dawley
18.
Int J Mol Med ; 30(2): 344-50, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22614038

ABSTRACT

Allergic inflammatory disease such as food allergy, asthma and atopic dermatitis are increasing worldwide. In this study, we investigated the effect of water extract of Sparassis crispa (WESC) Fr. (Aphyllophoromycetideae) on mast cell-mediated allergic inflammation and the possible mechanisms of action. WESC inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in mice. WESC decreased immunoglobulin E (IgE)-mediated passive cutaneous anaphylaxis. Additionally, WESC reduced histamine release and intracellular calcium in human mast cells activated by phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187. WESC decreased PMA and A23187-stimulated expression of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, inlerleukin (IL)-6 and IL-1ß. The inhibitory effect of WESC on pro-inflammatory cytokines was nuclear factor-κB, extracellular signal-regulated kinase and p38 mitogen-activated protein kinase-dependent. Our results suggest potential therapeutic application of WESC in allergic inflammatory diseases.


Subject(s)
Calcium/metabolism , Hypersensitivity/metabolism , Mast Cells/drug effects , Mast Cells/metabolism , Mitogen-Activated Protein Kinases/metabolism , NF-kappa B/metabolism , Polyporales/chemistry , Anaphylaxis/chemically induced , Anaphylaxis/drug therapy , Animals , Complex Mixtures/administration & dosage , Complex Mixtures/pharmacology , Cytokines/metabolism , Enzyme Activation/drug effects , Histamine Release/drug effects , Hypersensitivity/drug therapy , Hypersensitivity/immunology , Inflammation Mediators/metabolism , Male , Mast Cells/immunology , Mice , Mice, Inbred ICR , Rats , Rats, Sprague-Dawley , p-Methoxy-N-methylphenethylamine/adverse effects
19.
Toxicol Lett ; 210(1): 64-70, 2012 Apr 05.
Article in English | MEDLINE | ID: mdl-22322153

ABSTRACT

Perfluorooctanoic acid (PFOA) has unique physical and chemical characteristics, water and oil repellency, thermal stability, and surfactant properties. PFOA has been regularly found in the blood of animals and humans worldwide, and has become an increasing concern because of its adverse effects in immune system. However, the role of PFOA in the allergic inflammation is not well-known. To further extend the immunotoxicity of PFOA, we examined the role of PFOA on the mast cell-mediated allergic inflammation and studied the possible mechanism of action. PFOA dose- and time-dependently increased histamine release from mast cells and serum histamine by the induction of intracellular calcium. PFOA exacerbated the IgE-dependent local allergic reaction in the mouse allergy model. PFOA induced gene expression of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1ß, IL-6, and IL-8 in mast cells. The inducing effect of PFOA on the pro-inflammatory cytokines was nuclear factor-κB, p38 mitogen-activated protein kinase, and caspase-1 dependent. Furthermore, the activation of cyclooxygenase-2 by PFOA suggests the induction of allergic inflammatory mediators by the PFOA. Our findings provide evidence that PFOA, the known immunotoxic agent, induces mast cell-derived allergic inflammatory reactions by histamine release and expression of pro-inflammatory cytokines.


Subject(s)
Caprylates/toxicity , Drug Hypersensitivity/etiology , Fluorocarbons/toxicity , Histamine Release/drug effects , Inflammation Mediators/metabolism , Inflammation/chemically induced , Mast Cells/drug effects , Animals , Calcium/analysis , Caspase 1/metabolism , Cyclooxygenase 2/metabolism , Dose-Response Relationship, Drug , Drug Hypersensitivity/immunology , Enzyme Activation/drug effects , Female , Interleukin-1beta/biosynthesis , Interleukin-6/biosynthesis , Interleukin-8/biosynthesis , Male , Mast Cells/cytology , Mice , Mice, Inbred BALB C , Mice, Inbred ICR , Tumor Necrosis Factor-alpha/biosynthesis , p38 Mitogen-Activated Protein Kinases/metabolism
20.
Int J Mol Med ; 29(2): 303-10, 2012 Feb.
Article in English | MEDLINE | ID: mdl-22075758

ABSTRACT

In this study, we investigated the effect of a water extract of the ripe fruits of Rubus coreanus Miq. (Rosaceae) (RFRC) on mast cell-mediated allergic inflammation and studied the possible mechanism of action. Mast cell-mediated allergic disease is involved in many diseases such as anaphylaxis, rhinitis, asthma and atopic dermatitis. RFRC dose-dependently inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in mice. RFRC reduced the immunoglobulin E (IgE)-mediated local allergic reaction, passive cutaneous anaphylaxis. RFRC attenuated histamine release from rat peritoneal mast cells and human mast cells by the reduction of intracellular calcium. RFRC decreased the phorbol 12-myristate 13-acetate (PMA) and the calcium ionophore A23187 (PMACI)-stimulated expression and secretion of pro-inflammatory cytokines in human mast cells. The inhibitory effect of RFRC on cytokine production was nuclear factor (NF)-κB- and mitogen-activated protein kinase (MAPK)-dependent. In addition, RFRC suppressed the activation of caspase-1. Our findings provide evidence that RFRC inhibits mast cell-derived allergic inflammatory reactions, and for the involvement of calcium, NF-κB, MAPKs and caspase-1 in these effects. Furthermore, in vivo and in vitro anti-allergic inflammatory effects of RFRC provide affirmative proof of a possible therapeutic application of this agent in allergic inflammatory diseases.


Subject(s)
Anaphylaxis/immunology , Fruit/chemistry , Mast Cells/drug effects , Mast Cells/immunology , Plant Extracts/pharmacology , Rosaceae/chemistry , Anaphylaxis/chemically induced , Animals , Calcium/metabolism , Caspase 1/metabolism , Cell Line , Disease Models, Animal , Enzyme Activation/drug effects , Gene Expression/drug effects , Histamine Release/drug effects , Humans , Inflammation/immunology , Inflammation Mediators/metabolism , Male , Mice , Mitogen-Activated Protein Kinases/metabolism , NF-kappa B/metabolism , Rats , Rats, Sprague-Dawley , p-Methoxy-N-methylphenethylamine/adverse effects
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