An Evacuation Simulation for Hazard Analysis of Isolation at Sea during Passenger Ship Heeling.

The Crisis Management Manual is essential for safe and rapid evacuation in the event of an accident. However, the disaster response measures in the current ship evacuation manual are not systematic and are simple and impractical. In particular, the current instructions when the ship is heeling call for evacuation to the highest deck, in the opposite direction. This advice is dangerous, however, because it is isolated to a specific space, due to the walking limit angle according to the angle of heel during evacuation. This study focuses on the MV Sewol ferry accident on 16 April 2014, to evaluate the risk of isolation at sea when evacuating to the highest deck opposite in the direction of heeling when the ship was sinking. According to the initial angle of heel and various angular velocities, hazard maps were created for each safety factor to predict the risks for each situation, by performing a comprehensive evaluation of the safe and dangerous situations when evacuating to the highest deck. The problems and limitations of the current evacuation manuals and systems were identified, and the necessity of a new evacuation solution was presented.

The neuroprotective effect of staurosporine on mouse retinal ganglion cells after optic nerve injury.

This study aimed to investigate the neuroprotective properity of staurosporine (STS) and identify the neuroprotective mechanism of staurosporine in mouse retina ganglion cell after optic nerve injured. Mice (C57BL/6) were anaesthetised with a mixture of 5 mg/kg xylazine hydrochloride and 40 mg/kg tiletamine/zolazepam (Zoletil®). Optic nerves of the mice were crushed (Templeton JP et al., 2012). With micro-forceps, the bulbar conjunctiva was grasped and retracted, rotating the globe nasally. The exposed optic nerve was grasped approximately 1-3 mm from the globe with Dumont #N7 cross-action forceps for 10 s. One day after crushing, intravitreal injections of STS (500 nM) were administered using a Narishige IM-300 air pressure regulator. For analysing the change in ganglion cell number, the mice were allowed to live for 30 days, after which they were killed and the ganglion cell survival was assessed. A significant and marked loss of fluorescent spots was found after 30 days, with fewer 4',6-diamidino-2-phenylindole (DAPI)-expressing retinal ganglion cells (RGCs) remaining in the injured and phosphate buffered saline (PBS)-injected group than those in non-injured PBS-injected controls. However, RGC cell numbers dramatically increased in the STS intravitreal injection group. Moreover, degradation of nerve fibre (NF) was markedly reduced in the STS injection group compared with the injured and PBS-injected group by inducing astrocyte expression of Bcl-2. Our data suggested that injection of STS into the vitreous may have a potential therapeutic effect in retinal diseases such as glaucoma.