ABSTRACT
Angelicae Gigantis Radix (AGR) is one of the most widely used herbal medications. AGR is the dried root of Angelica gigas Nakai (Umbelliferae), which is known as Korean angelica. This study investigated the effects of AGR on osteoclast formation using primary bone marrow cells. TNF-alpha treatment increased tartrate-resistant acid phosphatase (Trap) positive cells and Trap activity in bone marrow cells. However, AGR significantly decreased both TNF-alpha-induced Trap positive cells and Trap activity. RT-PCR analyses revealed that AGR decreased mRNA levels of Trap and matrix metalloproteinase-9 in TNF-alpha-treated bone marrow cells. In addition, AGR decreased TNF-alpha-induced activation of NF-kappaB. These results suggest that AGR has an inhibitory effect on the formation of osteoclasts and its effect is partially related to the NF-kappaB pathway.
Subject(s)
Angelica/chemistry , Osteoclasts/drug effects , Plant Extracts/pharmacology , Acid Phosphatase/genetics , Animals , Bone Marrow Cells/cytology , Bone Marrow Cells/drug effects , Bone Marrow Cells/metabolism , Cells, Cultured , Enzyme-Linked Immunosorbent Assay , Isoenzymes/genetics , Male , Matrix Metalloproteinase 9/genetics , Mice , NF-kappa B/metabolism , Osteoclasts/cytology , Osteoclasts/metabolism , Plant Extracts/chemistry , RNA, Messenger/genetics , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Tartrate-Resistant Acid Phosphatase , Tumor Necrosis Factor-alpha/pharmacologyABSTRACT
BACKGROUND: Herbs have been used to treat stroke and coma patient in traditional Korean medicine (TKM). The novel decoction, Guhpoongchungsimhwan (GCH), was developed on the basis of clinical data and TKM theory. METHODS: We examined the neuroprotective effect of GCH on cerebral ischemia. The middle cerebral artery occlusion (MCAO) model was used to produce cerebral ischemia in Sprague-Dawley rats. Subjects were treated with GCH (50 or 200 mg/kg) or vehicle alone (controls) 0 and 2 hours after MCAO. The functional status was tested 24 hours after MCAO by neurological examination (clinical score) and by series of motor function tasks (foot placement and parallel bar crossing). RESULTS: The infarct volume was determined by 2,3,5-triphenyltetrazolium chloride staining 24 hours after surgery, and the expression of cyclooxygenase-2 was determined by immunohistochemistry. The clinical score of the GCH-treated group (200 mg/kg) was significantly lower than that of the control group (p<0.05), indicating fewer neurological deficits. The impairment of motor functions induced by MCAO was significantly reduced by the administration of GCH (p<0.05). The infarct volume was significantly smaller in the GCH-treated group (203.1 +/- 40.2 mm(3), p<0.05), as compared to the control group (377.8 +/- 32.6 mm(3)). The level of motor function in the GCH-treated group was associated with reduced infarct volume. In the analysis of immunohistochemistry, GCH treatment markedly inhibited the ischemia-induced expression of PTGS2 (prostaglandin-endoperoxidase synthase 2) or cyclooxygenase 2 (COX2), which plays an important role in ischemic neuronal cell death. CONCLUSION: The results showed that GCH reduced the infarct size and the functional deficits in MCAO rats.
Subject(s)
Brain Ischemia/prevention & control , Herbal Medicine/methods , Neuroprotective Agents/therapeutic use , Animals , Brain Infarction/etiology , Brain Infarction/prevention & control , Brain Ischemia/etiology , Brain Ischemia/metabolism , Brain Ischemia/pathology , Cyclooxygenase 2/metabolism , Disease Models, Animal , Dose-Response Relationship, Drug , Gene Expression/drug effects , Infarction, Middle Cerebral Artery/complications , Male , Motor Activity/drug effects , Motor Activity/physiology , Neurologic Examination/methods , Psychomotor Performance/drug effects , Rats , Rats, Sprague-Dawley , Time FactorsABSTRACT
Shiquandabutangjiaweibang (SDJ) is a traditional medicine prescription used for increasing body resistance against cancer. In the present study, the effect of SDJ extract on tumor metastasis and angiogenesis was evaluated. SDJ showed cytotoxicity against P388 (leukemia cells) and B16-F10 (murine melanoma cells) to 60% of control at 1 mg. SDJ significantly inhibited lung metastasis and also restored the number of platelets in C57BL/6 mice with thrombocytopenia induced by intravenous injection of B16-F10 cells. SDJ significantly disrupted chick embryonic angiogenesis in the chorioallantoic membrane (CAM). Interestingly, SDJ suppressed DNA topoisomerase I in a concentration-dependent manner. These results suggest that SDJ can be a potent inhibitor of metastasis and angiogenesis, at least in part, via regulation of topoisomerase I.
Subject(s)
DNA Topoisomerases, Type I/drug effects , DNA Topoisomerases, Type I/metabolism , Neoplasm Metastasis/prevention & control , Neovascularization, Pathologic/prevention & control , Plant Preparations/pharmacology , Animals , Cell Survival/drug effects , Chorioallantoic Membrane/drug effects , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor/methods , Humans , Leukemia P388/pathology , Leukemia P388/prevention & control , Lung Neoplasms/prevention & control , Lung Neoplasms/secondary , Male , Medicine, East Asian Traditional , Mice , Mice, Inbred C57BL , Neoplasm Transplantation/methods , Neoplasms, Experimental/drug therapy , Neoplastic Cells, Circulating/pathology , Plant Preparations/analysis , Plant Preparations/chemistry , Staining and Labeling , Thrombocytopenia/chemically induced , Thrombocytosis/chemically induced , Tumor Cells, CulturedABSTRACT
Citrus fruits have been known to reduce the proliferation of many cancer cells. The antiproliferative effects of Citrus reticulata Blanco (CR) extract, the immature tangerine peel, on human gastric cancer cell line SNU-668 were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, 4,6-diamidineo-2-phenylindole staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, reverse transcription-polymerase chain reaction expressions of BCL-2, BAX and CASP-3 genes, caspase-3 activity, and immunocytochemistry of caspase-3. From the results of the morphological and biochemical assays, CR (50 microg/ml) increased the apoptosis of human gastric cancer cells with typical apoptotic characteristics, including morphological changes of chromatin condensation and apoptotic body formation. CR (50 microg/ml) reduced the expression of BCL-2, whereas the expression of BAX and CASP-3 was increased compared with the control group. Furthermore, caspase-3 activity and caspase-3 protein expression in the CR-treated group was significantly increased compared with that in control group. These results suggest that CR may induce the apoptosis through the caspase-3 pathway in human gastric cancer cells.
