Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Virol Methods ; 108(1): 41-7, 2003 Mar.
Article in English | MEDLINE | ID: mdl-12565152

ABSTRACT

Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) were detected and differentiated in formalin-fixed, paraffin-embedded tissues from experimentally and naturally infected pigs by multiplex reverse transcription-nested polymerase chain reaction (RT-nPCR). The results of this new method were compared with in situ hybridization. A method based on xylene deparaffinization followed by proteinase K digestion yielded RNA of a suitable quality for reliable and consistent multiplex RT-nPCR analyses. PEDV and TGEV cDNAs were detected in jejunal tissues from experimentally and naturally infected pigs by multiplex RT-nPCR. Distinct positive signals for PEDV and TGEV were also detected in the same jejunal tissues by in situ hybridization. The rate of conformity between multiplex RT-nPCR and in situ hybridization was 100% for the detection of PEDV and TGEV in formalin-fixed paraffin-embedded jejunal tissues.


Subject(s)
Coronavirus/genetics , Coronavirus/isolation & purification , Transmissible gastroenteritis virus/genetics , Transmissible gastroenteritis virus/isolation & purification , Virology/methods , Animals , Base Sequence , Coronavirus/classification , Coronavirus M Proteins , DNA Primers/genetics , DNA, Viral/genetics , DNA, Viral/isolation & purification , Formaldehyde , Gastroenteritis, Transmissible, of Swine/epidemiology , Gastroenteritis, Transmissible, of Swine/virology , In Situ Hybridization , Jejunum/virology , Molecular Epidemiology , Paraffin Embedding , Reverse Transcriptase Polymerase Chain Reaction , Sensitivity and Specificity , Sus scrofa , Tissue Fixation , Transmissible gastroenteritis virus/classification , Viral Matrix Proteins/genetics , Virology/statistics & numerical data
SELECTION OF CITATIONS
SEARCH DETAIL
...