Volatile fatty acid-treated mixotrophic cultivation of lipid/carbohydrate-rich cyanobacterial species, Pseudanabaena mucicola GO0704, for the enhancement of biofuel production.

Cyanobacteria-derived biofuels can be helpful in achieving a circular bioeconomy. To increase the production of biodiesel/bioethanol from cyanobacterium, Pseudanabaena mucicola GO0704, mixotrophic cultivation using volatile fatty acid (VFA), a cheap organic carbon source, was performed. The treatment of butyric acid or acetic acid enhanced the cell growth, particularly, the dry weight of the butyric acid-treated cells was 2.30-fold higher than the control. The enhancement of the growth led to the increase of metabolite (i.e., lipid and carbohydrate) productions, resulting in high amount of biodiesel and bioethanol to be produced. Butyric acid was more effective compared to acetic acid and the productions of biodiesel (52.2 mg/L) and bioethanol (132.6 mg/L) from the butyric acid-treated P. mucicola GO0704 were 2.34- and 2.17-fold higher compared to the control, respectively. This study will provide a foundation to commercialize the cyanobacteria-based carbon-neutral fuels, and ultimately, achieve a circular bioeconomy.

Mixotrophic Cultivation of a Native Cyanobacterium, Pseudanabaena mucicola GO0704, to Produce Phycobiliprotein and Biodiesel.

Global warming has accelerated in recent decades due to the continuous consumption of petroleum-based fuels. Cyanobacteria-derived biofuels are a promising carbon-neutral alternative to fossil fuels that may help achieve a cleaner environment. Here, we propose an effective strategy based on the large-scale cultivation of a newly isolated cyanobacterial strain to produce phycobiliprotein and biodiesel, thus demonstrating the potential commercial applicability of the isolated microalgal strain. A native cyanobacterium was isolated from Goryeong, Korea, and identified as Pseudanabaena mucicola GO0704 through 16s RNA analysis. The potential exploitation of P. mucicola GO0704 was explored by analyzing several parameters for mixotrophic culture, and optimal growth was achieved through the addition of sodium acetate (1 g/l) to the BG-11 medium. Next, the cultures were scaled up to a stirred-tank bioreactor in mixotrophic conditions to maximize the productivity of biomass and metabolites. The biomass, phycobiliprotein, and fatty acids concentrations in sodium acetate-treated cells were enhanced, and the highest biodiesel productivity (8.1 mg/l/d) was achieved at 96 h. Finally, the properties of the fuel derived from P. mucicola GO0704 were estimated with converted biodiesels according to the composition of fatty acids. Most of the characteristics of the final product, except for the cloud point, were compliant with international biodiesel standards [ASTM 6761 (US) and EN 14214 (Europe)].

Elucidating the mechanisms underlying the cytotoxic effects of nano-/micro-sized graphene oxide on the microalgae by comparing the physiological and morphological changes in different trophic modes.

Understanding the cytotoxic mechanisms of environmental contaminants is important to estimate their environmental impacts and prepare guidelines for pollution control. Many studies have assessed the cytotoxic mechanisms of graphene oxide (GO), an emerging aquatic contaminant. However, in many cases, the effect of GO size and putative trophic modes of microalgae on cytotoxicity has been neglected, hindering complete understanding of the cytotoxic mechanisms of GO. In this study, the microalga Euglena gracilis cultivated under light (phototrophic) or dark (heterotrophic) conditions was exposed to two sizes of GO [nano-sized (N) and micro-sized (M)] for assessing the effect of GO on microalgal growth. The cytotoxic effect of GO was higher under phototrophic conditions than under heterotrophic conditions, suggesting that a major cytotoxic mechanism of GO is related to photosynthetic activity inhibition. Moreover, N-GO showed higher toxicity than M-GO. The morphological and physiological changes in N-GO- and M-GO-exposed E. gracilis were assessed to further elucidate the cytotoxic mechanisms. N-GO internalized the cells via endocytic activity/piercing, whereas M-GO partially attached to the cell surface and did not enter the cells. Moreover, N-GO and M-GO negatively affected the cells by inducing oxidative stress; the oxidative stress parameters were higher in N-GO-exposed cells than in M-GO-exposed cells.