Methane production of banana plant: Yield, kinetics and prediction models influenced by morphological parts, cultivars and ripening stages.

Banana trees and fruits with three ripening stages, including green, ripe, and overripe, of two cultivars, namely Nam wa and Hom were separated into different morphological parts for biogas yield determination. Specific methane yields (SMY) were significant different among banana parts (p ≤ 0.05). High non-structural carbohydrates and high non-lignocellulosic residual in substrates promoted high SMY. Pseudostem showed the highest share of energy yields among farm wastes which Nam wa cultivar provided higher energy potential than Hom. Peel presented the major energy source from fruit wastes which ripening stages did not have a significant effect on its SMY. Modified Gompertz model presented the best fit for methane production of most substrates. The SMY prediction models based on chemical constituents were developed to obtain conveniently used methane estimating tool which showed that a combination of lignin, hemicellulose, non-lignocellulosic residual, and crude fiber contents presented the highest performance for banana substrates.

Wickerhamiella nakhonpathomensis f.a. sp. nov., a novel ascomycetous yeast species isolated from a mushroom and a flower in Thailand.

Strains SU22T (TBRC 14875T) and FLA11.5, representing a novel anamorphic yeast species, were respectively isolated from a fruiting body of a Coprinus species and an inflorescence of a Coffea species collected in Thailand. Analysis of the sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions showed that the two strains differed by two nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and were identical in the ITS regions. Wickerhamiella drosophilae CBS 8459T was the most closely related species, but with 24-26 nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 24 nucleotide substitutions in the ITS regions. A phylogenetic analysis, based on the sequences of the D1/D2 domains, indicated that the two strains represented a species in the genus Wickerhamiella which was distinct from other recognized species of the genus. Therefore, the two strains were assigned as a novel species, for which we propose the name Wickerhamiella nakhonpathomensis f.a. sp. nov. The holotype is TBRC 14875T (isotype PYCC 8914T). The MycoBank number of the novel species is MB 840833.