Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 29
Filter
Add more filters










Publication year range
2.
Can Vet J ; 29(8): 647-53, 1988 Aug.
Article in English | MEDLINE | ID: mdl-17423101

ABSTRACT

Antibodies to Leptospira interrogans serovars hardjo and pomona were present in 8.3% and 0.5% of sera respectively, from adult female cattle in Alberta surveyed in 1984-85. Criterion for a positive serum sample was 50% agglutination at 1/100 dilution in the microscopic agglutination test. A positive herd contained one or more cows with positive serum. Prevalences were calculated on sample sizes that would give 80-95% reliability. Hardjo antibody prevalences and hardjo-positive herd prevalences were 0-53.9% and 0-83.3%, respectively, among 65 municipalities surveyed. Pomona prevalences by comparison were 0-3.4% and 0-11.7% respectively. Hardjo had increased significantly since 1980-82, and antibodies were found throughout the province. Pomona occurred mainly in southeastern Alberta, where it was isolated from cattle, swine and skunks. Hardjo was isolated only from cattle and it was found in many areas. Antibodies to icterohaemorrhagiae were present in 0.4% of sera from parts of Alberta surveyed in 1980; evidence of the presence of leptospires related to this serovar in bovine and porcine urinary tracts was obtained by immunofluorescence.

3.
J Wildl Dis ; 23(1): 86-91, 1987 Jan.
Article in English | MEDLINE | ID: mdl-3029443

ABSTRACT

Specimens from 28 wapiti (Cervus elaphus canadensis) were collected by hunters in southwestern Alberta in 1984. Various tests were performed to detect infections and conditions that could affect cattle sharing the range or cause disease in wapiti. Serum antibodies were present against leptospiral serovars autumnalis (25%), bratislava (4%), and icterohaemorrhagiae (8%), and the viruses of bovine virus diarrhea (52%), infectious bovine rhinotracheitis (45%), and parainfluenza type 3 (13%). No serological evidence of bovine respiratory syncytial virus, Brucella, Anaplasma, bluetongue virus, or epizootic hemorrhagic disease virus was found, nor were any lesions of vesicular diseases, necrotic stomatitis or nutritional myopathy evident. Focal interstitial nephritis and sarcocystosis were diagnosed histologically in 40% and 75%, respectively, of the wapiti tested. The prevalence of giant liver flukes (Fascioloides magna) was 50% and of lungworms (Dictyocaulus viviparus) 32%. Leptospiral serology on cattle in the area did not indicate that wapiti or cattle were a serious source of infection to each other. The giant liver fluke was the parasite most likely to be amplified by wapiti for cattle. Within the limits of this study, the results indicated that wapiti in the Waterton area do not pose a disease threat to the cattle with which they range, but periodic observational studies in these wapiti would be a useful means of early detection of any changes in the interspecies relationship.


Subject(s)
Bovine Virus Diarrhea-Mucosal Disease/epidemiology , Cattle Diseases/epidemiology , Deer/microbiology , Dictyocaulus Infections/epidemiology , Fascioloidiasis/epidemiology , Infectious Bovine Rhinotracheitis/epidemiology , Leptospirosis/veterinary , Paramyxoviridae Infections/veterinary , Sarcocystosis/veterinary , Alberta , Animals , Bovine Virus Diarrhea-Mucosal Disease/transmission , Cattle , Cattle Diseases/etiology , Cattle Diseases/microbiology , Cattle Diseases/parasitology , Dictyocaulus Infections/transmission , Fascioloidiasis/transmission , Infectious Bovine Rhinotracheitis/transmission , Leptospirosis/epidemiology , Leptospirosis/transmission , Parainfluenza Virus 3, Human , Paramyxoviridae Infections/epidemiology , Paramyxoviridae Infections/transmission , Sarcocystosis/epidemiology , Sarcocystosis/transmission
4.
Can Vet J ; 27(12): 512-6, 1986 Dec.
Article in English | MEDLINE | ID: mdl-17422729

ABSTRACT

Leptospiral antibodies were detected in unvaccinated cattle on a 17 000 hectare ranch in the arid southeast region of Alberta. Antibody to serovar hardjo was present before the breeding season in 7% of 42 yearling bulls, 86% of 29 two year old bulls and 5% of 519 cows. Pomona antibody was confined to 3.7% of the cows. Bulls were treated once with dihydrostreptomycin, 25 mg/kg. Bulls and cows were vaccinated twice at a six week interval, with pomona-hardjo-gripptotyphosa bacterin before breeding and cows were revaccinated the next year. Leptospires were demonstrated in urine, kidney and spinal fluid of vaccinated and treated cattle. New infections occurred on range in vaccinates. Eighteen months after the last vaccination, hardjo and pomona antibody prevalences in cows were 3.6 and 3.2% respectively. A group of 250 seronegative cows on the same ranch were not vaccinated. They remained seronegative throughout the 2.5 years of the study. These cows, in contrast to infected groups, were excluded from pastures adjacent to perimeter herds and grazing leases, and they were bred by artificial inseminstion. Rotation through pastures in common with infected groups, and exposure to seropositive heat detector bulls, did not result in seroconversion in these cows. The study showed the potential of range bulls to amplify and transmit hardjo infection, limitations to the value of treatment and vaccination with available agents, and the potential of management practices to maintain an uninfected herd in close proximity to cattle carrying hardjo infection.

5.
Can Vet J ; 27(11): 435-9, 1986 Nov.
Article in English | MEDLINE | ID: mdl-17422716

ABSTRACT

Abortion, premature calving, hemolytic anemia and fatal hematuria were associated with high levels (titer > 10(-4)) of antibody to Leptospira interrogans serovar hardjo and with isolation of hardjo in a herd of 265 beef cattle in the Great Clay Belt of northern Ontario. This herd was bred by artificial insemination, after heat detection by vasectomized bulls. The antibody prevalence rate in the herd was 54 to 60% over a five year period. The rate tended to reach 100% by age three years and to be below 5% in yearlings, which were raised in isolation from older cattle. Hardjo was isolated from the urine of a cow that aborted in the eighth month of pregnancy, and from kidneys of yearling steers which had been exposed to an older cow. Maternal antibody levels in calves paralleled those in their dams, protecting calves while they were being naturally exposed to infection, thus contributing to the achievement of balance between host and parasite. A controlled vaccination trial was conducted in 50 initially seronegative yearling steers and heifers. Serological response to vaccine was limited to a maximum agglutinin titer of 10(-2) in 8% of vaccinated cattle. Vaccination reduced the infection rate from 86% in the controls to 46% in the treated group, indirectly reducing the number of calves for which colostral antibody against hardjo would be available. A vaccination program was not implemented in the herd. Hardjo infection appeared to die out over a period of six years following the initial five year study period, with antibody prevalence falling from 60% to 0.7% and reactors persisting only in two eight year old cows. Decline in infection was coincident with changes in management which protected heifers from exposure to infection until their third pregnancy, and which probably lowered the reservoir of infection by increased culling from older age classes.

6.
Can Vet J ; 27(11): 440-2, 1986 Nov.
Article in English | MEDLINE | ID: mdl-17422717

ABSTRACT

Abortions occurred in 18% of 131 beef cows and heifers during two months, on a farm in southern Saskatchewan. The losses began two weeks after acute febrile illness and agalactia in a dairy cow to which the beef herd had been exposed. A diagnosis of Leptospira interrogans serovar pomona infection was made on the basis of serology in cows and the finding of leptospires in fetal tissues by fluorescent antibody test. Tentative diagnosis of infectious bovine rhinotracheitis delayed treatment and prophylaxis until infection attained high intensity in the herd and severe losses to the farmer occurred. Abortions ceased after vaccination against pomona and oxytetracycline treatment of pregnant cows, although chronic debility followed the acute phase of the disease in some cows. Recrudescence of infection was suspected four months later, when acute agalactia occurred in one cow and debility in calves and cows was recurring. Pomona infection was not proven, but dihydrostreptomycin treatment and revaccination were applied to the whole herd. Seroconversion and IgM antibody continued to indicate a persistent source of infection and susceptibility in a minority of the population one year after onset. The source of the original infection is believed to have been a carrier beef cow, or a dairy cow which was leptospiruric at the time of contact with the beef herd. With the exception of one aborted calf, no evidence of pomona infection was found outside the farm, in cattle or wild mammals tested serologically within a radius of 30 km, during one year following the outbreak.

7.
J Wildl Dis ; 22(4): 511-4, 1986 Oct.
Article in English | MEDLINE | ID: mdl-2845155

ABSTRACT

Sera from 210 pronghorns (Antilocapra americana) ranging in southeastern Alberta were tested for antibodies to disease agents present in indigenous cattle. No antibodies to Brucella abortus, Leptospira interrogans serovars pomona, hardjo, or grippotyphosa, or infectious bovine rhinotracheitis virus were found. Antibodies at prevalences of 43.8% and 49.2% were detected to bovine virus diarrhea (BVD) and parainfluenza type 3 (PI-3) viruses, respectively. The much higher prevalence of BVD virus antibodies in cattle than in pronghorns, and the occurrence of clinical bovine PI-3 infection in the study area, suggest that cattle may be a source of infection to the pronghorns.


Subject(s)
Antelopes/immunology , Antibodies, Bacterial/analysis , Antibodies, Viral/analysis , Artiodactyla/immunology , Alberta , Animals , Cattle , Cattle Diseases/microbiology , Diarrhea Viruses, Bovine Viral/immunology , Herpesvirus 1, Bovine/immunology , Leptospira interrogans/immunology , Parainfluenza Virus 3, Human
8.
J Wildl Dis ; 22(4): 475-8, 1986 Oct.
Article in English | MEDLINE | ID: mdl-3503132

ABSTRACT

The role of the red fox (Vulpes vulpes) in the epizootiology of leptospirosis in southwestern Ontario was investigated in 1973-1974. Leptospira interrogans serovar pomona (kennewicki by DNA analysis) was isolated from the kidneys of three of eight foxes tested. Severe hemorrhagic nephritis and interstitial nephritis were common to these foxes and to five others out of nine foxes examined. Autumnalis antibodies were detected at titers 10(-2) to 10(-5) in 12 of 100 fox sera. Pomona antibodies occurred in 6% of the sera, always accompanied by autumnalis antibodies, and at titers never exceeding the autumnalis titers. Cultural, serological, and pathological findings together indicated that the red fox could have been acting as an amplifier host, but not as a maintenance host, for pomona.


Subject(s)
Antibodies, Bacterial/analysis , Foxes/microbiology , Leptospira interrogans/immunology , Leptospirosis/veterinary , Animals , Leptospirosis/epidemiology , Leptospirosis/immunology , Leptospirosis/pathology , Nephritis/pathology , Nephritis/veterinary , Ontario
9.
Vet Rec ; 119(1): 11-2, 1986 Jul 05.
Article in English | MEDLINE | ID: mdl-3750750

ABSTRACT

From 1979 to 1984 approximately 2950 unprocessed frozen semen samples from nine Canadian artificial insemination centres were cultured for Mycoplasma bovis. M bovis was not detected in any of them.


Subject(s)
Insemination, Artificial/veterinary , Mycoplasma/isolation & purification , Semen Preservation/veterinary , Semen/microbiology , Animals , Canada , Cattle , Freezing , Male
10.
Can Vet J ; 27(4): 188-90, 1986 Apr.
Article in English | MEDLINE | ID: mdl-17422651

ABSTRACT

Fourteen abortions, stillbirths and neonatal deaths occurred over an interval of one month in crated sows in a farrow-to-finish swine operation. Leptospira interrogans serovar pomona (kennewicki by DNA analysis) was demonstrated by culture and fluorescent antibody test to be present in sows, piglets, boars, feeders, drinking water and skunks on the premises.Antibody was found in all clinically affected sows, at serum dilutions ranging from 1/800 to 1/25,600, and in all breeding boars at titers from 1/50 to 1/1600. Pomona antibody was present in 118 sow sera collected nine months before the outbreak, at a prevalence of 21.3%. Parvovirus infection in fetuses was intercurrent with the leptospirosis epizootic, despite vaccination for the former. Environmental contamination, feedback through skunks via drinking water, and dissemination through the piggery are discussed.

11.
Can Vet J ; 27(2): 78-81, 1986 Feb.
Article in English | MEDLINE | ID: mdl-17422627

ABSTRACT

Leptospirosis occurred in two veterinarians in Alberta, following their exposure to leptospires of domestic animal origin. The disease at onset resembled "flu" with fever, muscle and joint pain, and lassitude. It progressed through an extremely debilitating period with mild to severe hepatic and renal dysfunction, icterus and hemorrhage in one case, and cerebral meningitis in the other. Both patients were hospitalized for 11 to 14 days, where they responded to supportive and specific antibiotic and steroid therapy (penicillin G 10(6) IU q.i.d. and steroids, or tetracycline 500 mg q.i.d.). Diagnosis rested in one case on clinical signs and the observation of leptospires in blood and urine. In the other case, a tentative diagnosis of leptospirosis based on history and clinical signs was confirmed by serological test results and by the isolation of Leptospira interrogans serovar pomona from the patient's blood on day 6. Current occurrences of leptospirosis in man are reviewed. Convenient diagnostic methods, treatment and behavioural sequellae of leptospirosis are discussed.

12.
Am J Vet Res ; 47(1): 61-6, 1986 Jan.
Article in English | MEDLINE | ID: mdl-3004267

ABSTRACT

The genomes of North American strains of leptospires belonging to serogroups Mini and Sejroe were analyzed and compared with those of reference strains by cleavage with restriction endonucleases. The isolates selected for this study, when typed by the serologic method, were identified as serovars szwajizak, hardjo, and balcanica. However, the results of restriction endonuclease analysis (REA) indicated that a different classification existed. The 2 isolates typed as serovar szwajizak seem to be georgia by REA. Isolates belonging to serovars balcanica and hardjo had REA patterns that differed from both reference strains. Differences were not observed in the REA patterns between balcanica and hardjo isolates. All hardjo and balcanica isolates examined are suggested to be classified into a previously described hardjo, REA subtype hardjobovis. Using the enzyme Hha1, these isolates were subdivided into 3 subgroups. When examining the REA pattern of the 17 reference strains in serogroup Sejroe, 3 identical pairs were observed: wolffi and roumanica; sejroe and polonica; and istrica and nyanza. The REA again indicated that it will be a valuable method for the classification of leptospires.


Subject(s)
DNA, Bacterial/analysis , Genes, Bacterial , Leptospira/classification , Serotyping , DNA Restriction Enzymes , Leptospira/genetics
13.
J Wildl Dis ; 22(1): 42-7, 1986 Jan.
Article in English | MEDLINE | ID: mdl-3951060

ABSTRACT

Samples of muscle from 4,773 specimens of 18 species of wild mammals from Ontario were examined for Trichinella. One of 12 mink (Mustela vison), 83 of 1,821 fisher (Martes pennanti) and 68 of 1,980 marten (Martes americana) had T. spiralis. Prevalences of infections by Trichinella were determined for fisher and marten from the Algonquin region, over a 10-yr period. Prevalences ranged from 0.9-9.2% in fisher and 1.3-8.7% in marten indicating that the parasite is well-established in the region. Prevalences of Trichinella increased with age of both fisher and marten. Intensities determined for the 1981-1982 sample ranged from 0.4-15.8 larvae/g for fisher and 22.4-159.7 larvae/g for marten. Higher intensities were not correlated with older hosts. Fisher and marten appeared to be the key hosts maintaining Trichinella in the Algonquin region, but transmission dynamics were unclear. Transmission may include an unidentified small rodent or other host and natural carrion-feeding.


Subject(s)
Animal Population Groups/parasitology , Animals, Wild/parasitology , Trichinellosis/veterinary , Animals , Disease Reservoirs , Female , Male , Ontario , Trichinella/isolation & purification , Trichinellosis/epidemiology , Trichinellosis/transmission
15.
Can Vet J ; 26(10): 328-32, 1985 Oct.
Article in English | MEDLINE | ID: mdl-17422584

ABSTRACT

An outbreak of leptospirosis due to Leptospira interrogans serovar hardjo in the South Okanagan District of British Columbia was investigated. The infection was associated primarily with bulls, but serovar hardjo was isolated from both bulls and cows at slaughter. Kidney and cerebrospinal fluid were found to contain leptospires, independently of the presence and level of serum agglutinins. Treatment of a bull twice in six months with dihydrostreptomycin failed to diminish an agglutinin titer (1/200) which persisted for two years without reexposure of the bull. A serological survey of cull cows sold through a central auction mart revealed the presence of hardjo agglutinins in 15.4% of 1300 sera representing 163 herds in 20 locations. Thirty percent of these herds contained reactor cattle. The number of premises from which reactor cattle came in a given locality varied from 4% to 67.7%. Measures to control leptospirosis in the study are suggested.

16.
Can Vet J ; 26(9): 270-4, 1985 Sep.
Article in English | MEDLINE | ID: mdl-17422570

ABSTRACT

The investigations described were designed to identify the cause of serological reactions to Leptospira interrogans serovars hardjo and sejroe in Canadian cattle, and to confirm by culture a diagnosis of leptospirosis in cases of reproductive failure and atypical mastitis.Leptospires were detected in ten of 64 urine cultures, nine of 18 kidney cultures, and one of nine cerebrospinal fluid cultures. Twelve strains were purified. All were placed in the serogroup which contains serovars hardjo and sejroe. The nine strains which were fully serotyped were considered to be identical with serovar hardjo strain hardjoprajitno. Hardjo was isolated from cattle in the presence or absence of clinical disease and of antibody detectable by the microscopic agglutination test. Hardjo antigen was more sensitive than sejroe in detecting agglutinins in 58% of actively infected cattle and equal in 25%, as shown by comparative serum titrations.

17.
Can Vet J ; 26(8): 235-6, 1985 Aug.
Article in English | MEDLINE | ID: mdl-17422559
18.
Can Vet J ; 26(5): 164-8, 1985 May.
Article in English | MEDLINE | ID: mdl-17422531

ABSTRACT

A survey of 930 ovine sera and kidneys from 33 sheep was conducted to assess the rate of leptospiral infection in sheep slaughtered in Alberta. Sera were tested for the presence of agglutinins to indigenous serovars of Leptospira interrogans. Kidneys with gross lesions were examined for the presence of leptospires by means of an indirect fluorescent antibody test (FAT) and by culture. Antibodies to serovars pomona and hardjo were present at rates of 1.0% and 0.4%, respectively, in sheep from Saskatchewan, Alberta and British Columbia. Sera from 120 feedlot lambs shipped from Oregon reacted to serovars pomona, hardjo and grippotyphosa at rates of 1.7%, 61.7% and 59.1%, respectively. Fluorescent antibody test detected serovars (presumptively) hardjo in 52% of Oregon feedlot lambs and grippotyphosa in 32% of the same group, a finding supported by the isolation of both these serovars from a pool of two fluorescent antibody test-positive kidneys. The grippotyphosa strain was highly virulent for hamsters, producing intense icterus and death. Leptospires, presumptively serovar grippotyphosa were demonstrated by fluorescent antibody test in one Alberta lamb kidney. The possibility of spreading leptospirosis by movement of breeding stock through public facilities and by assembling lambs in feedlots is discussed.

19.
J Clin Microbiol ; 21(4): 585-7, 1985 Apr.
Article in English | MEDLINE | ID: mdl-2985648

ABSTRACT

The genomes of leptospiral field isolates belonging to serogroup Pomona were analyzed and compared with those of type strains by cleavage with restriction endonucleases. This new classification method shows differences among these organisms not indicated by the conventional serological typing method. No differences were observed among isolates from the United States, Canada, and New Zealand. Although all isolates selected for this study had been serologically typed as belonging to serovar pomona, the restriction endonuclease analysis indicates that they belong to serovar kennewicki. kennewicki, a serovar of North American origin, has recently been eliminated from the official serovar list because it was found to be indistinguishable from serovar pomona by the serological method.


Subject(s)
DNA, Bacterial/analysis , Leptospira/classification , DNA Restriction Enzymes/pharmacology , Leptospira/analysis
20.
Can J Comp Med ; 47(3): 375-8, 1983 Jul.
Article in English | MEDLINE | ID: mdl-6315201

ABSTRACT

A group of 62 beef calves, born and raised in an institutional herd, were transferred at nine months of age to a commercial feedlot where they remained until slaughter seven months later. Clinical, immunological and microbiological monitoring was carried out during this period. No serious clinical illness occurred. One hundred percent seroconversion to bovine virus diarrhea virus took place after introduction of the calves into the feedlot as well as almost complete (59/62) seroconversion to bovine herpesvirus 1, a proportion of which could be related to a single vaccination. Significant increases in recoveries of Mycoplasma spp. from nasal swabs also occurred in the feedlot. At slaughter, the lungs of all animals were recovered and examined for pathological lesions: 23 were completely normal and 39 showed minor histological changes chiefly characterized by areas of lobular to sublobular atelectasis. For this group of calves, no relationship was found between the presence of potential pathogens in nasal mucus and the occurrence of lesions in the lung. The serological results are discussed in terms of vaccinations and other known events that occurred during the study period.


Subject(s)
Cattle Diseases/pathology , Cattle/immunology , Lung/pathology , Nasal Mucosa/microbiology , Animals , Antibodies, Bacterial/analysis , Antibodies, Viral/analysis , Cattle/microbiology , Diarrhea Viruses, Bovine Viral/immunology , Female , Herpesvirus 1, Bovine/immunology , Male , Mycoplasma/immunology , Mycoplasma/isolation & purification , Parainfluenza Virus 3, Human/immunology , Pasteurella/immunology , Pasteurella/isolation & purification , Species Specificity
SELECTION OF CITATIONS
SEARCH DETAIL
...