ABSTRACT
The most promising anti-tumor agent developed in the past three decades is Taxol. It is proven to be effective against many cancers. It is necessary to isolate pharmacologically potent endophytic microbial strains from medicinal plants with special reference to Taxol production. In the current study, endophytic fungi were isolated from the bark of the medicinal plant, Salacia oblonga. The isolated endophytes were identified morphologically, and further characterized by ITS-PCR using genomic DNA samples, later the products were sequenced for identification and phylogenetic linkage mapping. The samples were screened for the potential to produce Taxol or taxanes, employing PCR. The resulted data have been sequenced to confirm the presence of the two genes implicated in Taxol biosynthesis, 10-deacetylbaccatin III-10-O-acetyl transferase (DBAT) and C-13 phenylpropanoid side chain-CoA acyltransferase (BAPT). Seven samples showed the amplicons of DBAT gene and one showed the amplicons of BAPT gene. Sequencing of these products was carried out, of which one sample has revealed sequence homology to the original DBAT gene from Taxus. The present work confirms and substantiates the potential of genomic mining approach to discover novel Taxol-producing endophytic fungi.
ABSTRACT
A paratesticular rhabdomyosarcoma occurred in a child with factor IX deficiency and neurofibromatosis, illustrating the need to consider carefully the various etiologic possibilities of a soft-tissue mass in a child with neurofibromatosis and/or a bleeding disorder.
Subject(s)
Hemophilia B/complications , Neoplasms, Multiple Primary/blood , Neurofibromatosis 1/blood , Rhabdomyosarcoma/blood , Diseases in Twins , Genital Neoplasms, Male/blood , Humans , Infant , Male , Skin Neoplasms/bloodABSTRACT
Neural crest and nonneural crest tumors occur frequently in neurofibromatosis (NF). We report one case of NF and recurrent malignant fibrous histiocytoma, a tumor that is uncommon in childhood, and another case of the concomitant occurrence of NF, hemophilia B, and a paratesticular rhabdomyosarcoma.
Subject(s)
Neoplasms, Multiple Primary , Neurofibromatosis 1/genetics , Skin Neoplasms/genetics , Adolescent , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Femoral Neoplasms/diagnosis , Femoral Neoplasms/therapy , Hemophilia B/complications , Histiocytoma, Benign Fibrous/diagnosis , Histiocytoma, Benign Fibrous/therapy , Humans , Infant , Male , Rhabdomyosarcoma/diagnosis , Rhabdomyosarcoma/drug therapy , Testicular Neoplasms/diagnosis , Testicular Neoplasms/drug therapySubject(s)
Lead Poisoning , Child , Child, Preschool , Humans , Infant , Lead Poisoning/blood , Lead Poisoning/diagnosis , Lead Poisoning/drug therapySubject(s)
Burkitt Lymphoma/diagnosis , Leukemia, Lymphoid/diagnosis , Adolescent , Bone Marrow/pathology , Burkitt Lymphoma/complications , Burkitt Lymphoma/drug therapy , Child , Child, Preschool , Female , Histiocytes/cytology , Humans , Leukemia, Lymphoid/complications , Leukemia, Lymphoid/drug therapy , Lymph Nodes/pathology , Male , Phagocytosis , Prednisone/therapeutic useABSTRACT
A ring chromosome 6 has been identified by GTG-banding in a male with microcephaly, growth retardation, seizures, epicanthus, hypertelorism, micrognathia, and other congenital anomalies. Cytogenetic studies indicate the instability of the ring chromosome. The most common findings in subjects with ring 6 include: profound to moderate mental retardation, microcephaly, prenatal growth failure, retarded bone age, epicanthal folds, flat nasal bridge, short neck, ears low-set or malformed, microphthalmia, and micrognathia. Linkage studies, including HLA, are consistent with reported maps of chromosome 6.
Subject(s)
Chromosome Aberrations , Chromosome Disorders , Chromosomes, Human, 6-12 and X , Growth Disorders/genetics , Intellectual Disability/genetics , Microcephaly/genetics , Seizures/genetics , Child, Preschool , Chromosome Banding , Humans , Karyotyping , Male , Phenotype , SyndromeABSTRACT
A 12-year-old boy being examined for vague chest pains was found to be suffering from acid maltase deficiency. Unlike previously reported cases in which vacuolization was most commonly noted in type I fibers, type II fibers were selectively involved in this patient and were atrophic Type I fibers were spared, or occasionally contained one or more small globular structures consisting of large, complex aggregates of lysosomal profiles.
