ABSTRACT
A short formal synthesis of squalamine is described, utilizing the biotransformation product 2, which is available in one step from commercially available 3-keto-23,24-bisnorchol-4-en-22-ol (1). Regioselective C-22 oxidation and C-24 sulfation of the corresponding alcohols in the presence of a free C-7 alcohol make for an efficient preparation of squalamine intermediate 11.
Subject(s)
Angiogenesis Inhibitors/chemical synthesis , Cholestanols/chemical synthesis , Hydroxylation , Mitosporic Fungi/metabolism , Norsteroids/chemistry , Norsteroids/metabolismABSTRACT
Seven new aminosterols related to squalamine (8) were isolated from the liver of the dogfish shark Squalus acanthias. Their structures (1-7) were determined using spectroscopic methods, including 2D NMR and HRFABMS. These aminosterols possess a relatively invariant cholestane skeleton with a trans AB ring junction, a spermidine or spermine attached equatorially at C3, and a steroidal side-chain that may be sulfated. The structure of the lone spermine conjugate, 7 (MSI-1436), was confirmed by its synthesis from (5alpha,7alpha, 24R)-7-hydroxy-3-ketocholestan-24-yl sulfate. Some members of this family of aminosterols exhibit a broad spectrum of antimicrobial activity comparable to squalamine.
Subject(s)
Anti-Bacterial Agents/chemistry , Dogfish/metabolism , Sterols/chemistry , Animals , Anti-Bacterial Agents/isolation & purification , Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Chromatography, High Pressure Liquid , Liver/chemistry , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Spectrometry, Mass, Fast Atom Bombardment , Sterols/isolation & purification , Sterols/pharmacologyABSTRACT
The novel aminosterol, squalamine, inhibits angiogenesis and tumor growth in multiple animal models. This effect is mediated, at least in part, by blocking mitogen-induced proliferation and migration of endothelial cells, thus preventing neovascularization of the tumor. Squalamine has no observable effect on unstimulated endothelial cells, is not directly cytotoxic to tumor cells, does not alter mitogen production by tumor cells, and has no obvious effects on the growth of newborn vertebrates. Squalamine was also found to have remarkable effects on the primitive vascular bed of the chick chorioallantoic membrane, which has striking similarities to tumor capillaries. Squalamine may thus be well suited for treatment of tumors and other diseases characterized by neovascularization in humans.
Subject(s)
Anticarcinogenic Agents/pharmacology , Neovascularization, Physiologic/drug effects , Animals , Cell Division/drug effects , Cell Movement/drug effects , Chick Embryo/drug effects , Cholestanols/pharmacology , Collagen , Cornea , Corneal Neovascularization/prevention & control , Drug Combinations , Endothelial Growth Factors/metabolism , Endothelial Growth Factors/pharmacology , Endothelium, Vascular/drug effects , Eye Neoplasms/prevention & control , Fibroblast Growth Factor 2/pharmacology , Glioma/drug therapy , Glioma/pathology , Laminin , Lymphokines/drug effects , Lymphokines/metabolism , Lymphokines/pharmacology , Mice , Mice, Inbred BALB C , Platelet-Derived Growth Factor/pharmacology , Proteoglycans , Rabbits , Rats , Rats, Inbred F344 , Transplantation, Heterologous , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth FactorsABSTRACT
The diazabicyclic amino acid phosphonate 15, [2-(8,9-dioxo-2,6-diazabicyclo[5.2.0]non-1(7)-en-2-yl)ethyl]phosphonic acid, was identified as a potent NMDA antagonist. It contains the alpha-amino acid bioisostere 3,4-diamino-3-cyclobutene-1,2-dione and an additional ring for conformational rigidity. Compound 15 was as potent as CGS-19755 (5) in the [3H]CPP binding assay, the stimulated [3H]TCP binding assay, and the NMDA-induced lethality model in mice. A single bolus dose of compound 15, administered intravenously following permanent occlusion of middle cerebral artery (MCA) in the rat, reduced the size of infarcted tissue by 57%. Structure-activity relationship (SAR) studies have indicated that the six- and eight-membered ring derivatives had diminished activity and that the two-carbon side chain length was optimum for NMDA receptor affinity. Substitution on the ring was found to be counterproductive in the case of sterically demanding dimethyl groups and of no consequence in the case of an H-bonding hydroxyl group. Replacement of the phosphonic acid group by either a carboxylic acid or a tetrazole group was unproductive. The potent bicyclic NMDA antagonists were synthesized efficiently by virture of their achiral nature and the ease of vinylgous amide formation from squaric acid esters. Compound 15, being a unique NMDA antagonist structural type with a favorable preclinical profile, may offer advantages over existing NMDA antagonists for the treatment of neurological disorders such as stroke and head trauma. Compound 15 is currently under clinical evaluation as a neuroprotective agent for stroke.
Subject(s)
Bridged Bicyclo Compounds, Heterocyclic/chemical synthesis , Cyclobutanes/chemistry , Excitatory Amino Acids/chemistry , N-Methylaspartate/antagonists & inhibitors , Animals , Azabicyclo Compounds , Bridged Bicyclo Compounds, Heterocyclic/chemistry , Cyclobutanes/metabolism , Disease Models, Animal , Excitatory Amino Acid Antagonists/chemistry , Excitatory Amino Acid Antagonists/metabolism , Excitatory Amino Acids/metabolism , Ischemic Attack, Transient/metabolism , Ischemic Attack, Transient/pathology , Mice , Models, Chemical , Organophosphonates , Pipecolic Acids/chemistry , Pipecolic Acids/metabolism , Piperazines/chemistry , Piperazines/metabolism , RatsABSTRACT
Analogs of the aminosterol antimicrobial agent squalamine have been synthesized beginning from hyodeoxycholic acid. After carboxylic acid esterification and oxidation of both alcohol functions to ketones, the A/B ring junction was converted from cis to trans by acid-catalyzed isomerization. Different polyamines were added to the 3-keto group by reductive amination, yielding both the 3 alpha and 3 beta addition products. The synthetic products exhibited potent, broad-spectrum antimicrobial activity similar to that of the parent compound. Changing the identity of the polyamine or the stereochemistry of addition has little effect upon antimicrobial activity but appears to change the selectivity of the agents. The analogs are synthesized with high yield from inexpensive starting materials and are promising alternatives to squalamine as potential antibiotics.
Subject(s)
Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/pharmacology , Deoxycholic Acid/chemistry , Anti-Bacterial Agents/chemistry , Cholestanols/chemistry , Cholestanols/pharmacology , Cholic Acids/chemical synthesis , Cholic Acids/chemistry , Cholic Acids/pharmacology , Detergents/chemistry , Ethylenediamines/chemical synthesis , Ethylenediamines/chemistry , Ethylenediamines/pharmacology , Microbial Sensitivity Tests , Pseudomonas aeruginosa/drug effects , Spermine/analogs & derivatives , Spermine/chemical synthesis , Spermine/chemistry , Spermine/pharmacology , Staphylococcus aureus/drug effects , Structure-Activity RelationshipABSTRACT
In this report, a novel bioisostere of the alpha-amino acid, 3,4-diamino-3-cyclobutene-1,2-dione, has been incorporated into a series of compounds which are NMDA antagonists. These compounds, which are achiral and easily prepared, demonstrated good affinity at the NMDA receptor by their ability to displace [3H]CPP binding in vitro. In particular, the phosphonic acid 24 provided protection against NMDA-induced lethality in mice equivalent to 2-amino-7-phosphonoheptanoic acid (5). This was considered an encouraging result in lieu of the fact that 24, like 5, lacks the conformational rigidity of the more potent NMDA antagonists. In addition, analogs that incorporate the 1,2,4-oxadiazolidine-3,5-dione heterocycle of quisqualic acid and the unsaturation of kainic acid were prepared to explore selectivity at the non-NMDA receptor subtypes.
Subject(s)
2-Amino-5-phosphonovalerate/analogs & derivatives , Carboxylic Acids/chemical synthesis , N-Methylaspartate/antagonists & inhibitors , 2-Amino-5-phosphonovalerate/chemistry , 2-Amino-5-phosphonovalerate/toxicity , Animals , Binding Sites/drug effects , Carboxylic Acids/chemistry , Carboxylic Acids/toxicity , Male , Mice , Receptors, Glutamate/drug effects , Receptors, Glutamate/metabolism , Receptors, Kainic Acid , Receptors, N-Methyl-D-Aspartate/drug effects , Receptors, N-Methyl-D-Aspartate/metabolism , Stereoisomerism , Structure-Activity RelationshipABSTRACT
A series of substituted 2-pyridinecarbothioamides was synthesized and evaluated for gastric mucosal protectant activity in the rat. Out of this investigation N-(3,5-difluorophenyl)-2- pyridinecarbothioamide (23, AY-31,574) was identified. This compound was much more potent than sucralfate and ranitidine against ethanol-induced lesions. Compound 23 was equipotent with ranitidine against gastric injury caused by stress. Unlike ranitidine, 23 was found to be devoid of antisecretory activity in the pylorus-ligated rat model, making it a selective mucosal protectant. Such a potent selective mucosal protectant may provide a novel clinical approach in treating ulcers.
