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1.
Biochemistry ; 40(20): 6124-31, 2001 May 22.
Article in English | MEDLINE | ID: mdl-11352750

ABSTRACT

The proton-translocating NADH:ubiquinone oxidoreductase of respiratory chains (complex I) contains one flavin mononucleotide and five EPR-detectable iron-sulfur clusters as redox groups. Because of the number of conserved motifs typical for binding iron-sulfur clusters and the high content of iron and acid-labile sulfide of complex I preparations, it is predicted that complex I contains additional clusters which have not yet been detected by EPR spectroscopy. To search for such clusters, we used a combination of UV/vis and EPR spectroscopy to study complex I from Neurospora crassa and Escherichia coli adjusted to distinct redox states. We detected a UV/vis redox difference spectrum characterized by negative absorbances at 325 and 425 nm that could not be assigned to the known redox groups. Redox titration was used to determine the pH-independent midpoint potential to be -270 mV, being associated with the transfer of two electrons. Comparison with UV/vis difference spectra obtained from complex I fragments and related enzymes showed that this group is localized on subunit Nuo21.3c of the N. crassa or NuoI of the E. coli complex I, respectively. This subunit (the bovine TYKY) belongs to a family of 8Fe-ferredoxins which contain two tetranuclear iron-sulfur clusters as redox groups. We detected EPR signals in a fragment of complex I which we attribute to the novel FeS clusters of complex I.


Subject(s)
Ferredoxins/chemistry , NADH, NADPH Oxidoreductases/chemistry , Animals , Cattle , Clostridium/enzymology , Cupriavidus necator/enzymology , Electron Spin Resonance Spectroscopy , Electron Transport Complex I , Escherichia coli/enzymology , Hydrogenase/chemistry , Iron-Sulfur Proteins/chemistry , Methanosarcina barkeri/enzymology , Neurospora crassa/enzymology , Oxidation-Reduction , Oxidoreductases/chemistry , Oxygen/chemistry , Spectrophotometry, Ultraviolet
2.
Biochim Biophys Acta ; 1459(2-3): 305-9, 2000 Aug 15.
Article in English | MEDLINE | ID: mdl-11004444

ABSTRACT

The proton-pumping NADH:ubiquinone oxidoreductase is the first of the respiratory chain complexes in many bacteria and mitochondria of most eukaryotes. The bacterial complex consists of 14 different subunits. Seven peripheral subunits bear all known redox groups of complex I, namely one FMN and five EPR-detectable iron-sulfur (FeS) clusters. The remaining seven subunits are hydrophobic proteins predicted to fold into 54 alpha-helices across the membrane. Little is known about their function, but they are most likely involved in proton translocation. The mitochondrial complex contains in addition to the homologues of these 14 subunits at least 29 additional proteins that do not directly participate in electron transfer and proton translocation. A novel redox group has been detected in the Neurospora crassa complex, in an amphipathic fragment of the Escherichia coli complex I and in a related hydrogenase and ferredoxin by means of UV/Vis spectroscopy. This group is made up by the two tetranuclear FeS clusters located on NuoI (the bovine TYKY) which have not been detected by EPR spectroscopy yet. Furthermore, we present evidence for the existence of a novel redox group located in the membrane arm of the complex. Partly reduced complex I equilibrated to a redox potential of -150 mV gives a UV/Vis redox difference spectrum that cannot be attributed to the known cofactors. Electrochemical titration of this absorption reveals a midpoint potential of -80 mV. This group is believed to transfer electrons from the high potential FeS cluster to ubiquinone.


Subject(s)
NADH, NADPH Oxidoreductases/chemistry , Electrochemistry , Electron Spin Resonance Spectroscopy , Electron Transport Complex I , Escherichia coli , Iron-Sulfur Proteins/chemistry , Mitochondria/chemistry , Neurospora crassa , Oxidation-Reduction , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform Infrared
3.
Biochim Biophys Acta ; 1365(1-2): 215-9, 1998 Jun 10.
Article in English | MEDLINE | ID: mdl-9693737

ABSTRACT

The proton-pumping NADH:ubiquinone oxidoreductase is the first complex in the respiratory chains of many purple bacteria and of mitochondria of most eucaryotes. The bacterial complex consists of 14 different subunits. The mitochondrial complex contains at least 29 additional proteins that do not directly participate in electron transfer and proton translocation. We analysed electron micrographs of isolated and negatively stained complex I particles from Escherichia coli and Neurospora crassa and obtained three-dimensional models of both complexes at medium resolution. Both have the same L-shaped overall structure with a peripheral arm protruding into the aqueous phase and a membrane arm extending into the membrane. The two arms of the bacterial complex are only slightly shorter than those of the mitochondrial complex although the protein mass of the former is only half of that of the latter. The presence of a novel redox group in the membrane arm of the complex is discussed. This group has been detected in the N. crassa complex by means of UV-visible spectroscopy. After reduction with an excess of NADH and reoxidation by the lactate dehydrogenase reaction, a reduced-minus-oxidized difference spectrum was obtained that cannot be attributed to the known cofactors flavin mononucleotide (FMN) and the FeS clusters N1, N2, N3 and N4. Due to its positive midpoint potential the novel group is believed to transfer electrons from the FeS clusters to ubiquinone. Its role in proton translocation is discussed.


Subject(s)
NAD(P)H Dehydrogenase (Quinone)/metabolism , Computer Simulation , Electron Spin Resonance Spectroscopy , Escherichia coli , Models, Molecular , Neurospora crassa , Oxidation-Reduction , Rhodobacter capsulatus , Spectrophotometry, Ultraviolet , Thermus thermophilus
4.
Biofactors ; 8(3-4): 177-86, 1998.
Article in English | MEDLINE | ID: mdl-9914816

ABSTRACT

The proton-translocating NADH:ubiquinone oxidoreductase of mitochondria (complex I) is a large L-shaped multisubunit complex. The peripheral matrix arm contains one FMN and a number of iron-sulfur (FeS) clusters and is involved in NADH oxidation and electron transfer to the membrane intrinsic arm. There, following a yet unknown mechanism, the redox-driven proton translocation and the ubiquinone reduction take place. Redox groups that would be able to link electron transfer with proton translocation have not been found so far in the membrane arm. We searched for such groups in complex I isolated from Neurospora crassa. Under anaerobic conditions, the preparation was analyzed in different redox states by means of UV/VIS and EPR spectroscopy. Absorption bands in the UV/VIS redox difference spectra were found which cannot be attributed to the FMN or the EPR detectable FeS clusters. The existence of two novel groups is postulated and their possible locations in the electron pathway and their roles in proton translocation are discussed.


Subject(s)
Mitochondria/enzymology , NAD(P)H Dehydrogenase (Quinone)/chemistry , Oxidation-Reduction , Spectrophotometry/methods , Electron Spin Resonance Spectroscopy , Flavin Mononucleotide/analysis , Iron-Sulfur Proteins/analysis , Kinetics , L-Lactate Dehydrogenase/metabolism , NAD/metabolism , NAD/pharmacology , NAD(P)H Dehydrogenase (Quinone)/isolation & purification , NAD(P)H Dehydrogenase (Quinone)/metabolism , Neurospora crassa/enzymology , Neurospora crassa/ultrastructure , Spectrophotometry, Ultraviolet/methods
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