ABSTRACT
CONTEXT: Although the apolipoprotein E (APOE) ϵ4 allele is a major genetic risk factor for late-onset Alzheimer disease, its effect on hippocampal function during episodic memory is controversial because studies have yielded mixed results. The age of the studied cohorts may contribute to this apparent inconsistency: activation for ϵ4 carriers tends to be increased in studies of older adults but decreased in some studies of younger adults. Consistent with differential age effects, research in transgenic mice suggests that the ϵ4 allele may particularly affect the aging process. OBJECTIVE: To define the interactions of age and this allelic variation on brain activation during episodic memory across adult life in healthy individuals. DESIGN: Functional magnetic resonance imaging (fMRI) using an episodic memory paradigm to test for differences in neuroactivation across APOE genotypes and age groups. SETTING: A federal research institute. PARTICIPANTS: Healthy white volunteers (APOE ϵ3 homozygotes and ϵ2 and ϵ4 heterozygotes) completed the fMRI task (133 volunteers aged 19-77 years). MAIN OUTCOME MEASURE: Memory-related regional blood oxygenation level-dependent (BOLD) activation. RESULTS: Genotype affected the pattern of change in hippocampal BOLD activation across the adult lifespan: older age was associated with decreased activation in ϵ2 carriers and, to a lesser extent, in ϵ3 homozygotes, but this pattern was not observed in ϵ4 carriers. Among young participants, ϵ4 carriers had less hippocampal activation compared with ϵ3 homozygotes despite similar task performance. CONCLUSIONS: The findings support the hypothesis that aging and APOE allele status have interacting effects on the neural substrate of episodic memory and lend clarification to disparities in the literature. The stepwise decrease in activation with age found among genotype groups resembles the order of susceptibility to Alzheimer disease, suggesting a compensatory neurobiological mechanism in older asymptomatic ϵ4 carriers.
Subject(s)
Alzheimer Disease , Apolipoprotein E3/genetics , Apolipoprotein E4/genetics , Brain Mapping/methods , Hippocampus , Memory, Episodic , Adult , Age Factors , Aged , Aging/genetics , Alzheimer Disease/genetics , Alzheimer Disease/psychology , Female , Genetic Carrier Screening , Genetic Predisposition to Disease , Hippocampus/metabolism , Hippocampus/physiopathology , Humans , Magnetic Resonance Imaging/methods , Male , Middle Aged , Neuropsychological Tests , Risk FactorsABSTRACT
Williams syndrome (WS), caused by microdeletion of some 21 genes on chromosome 7q11.23, is characterized by dysmorphic features, mental retardation or learning difficulties, elastin arteriopathy, and striking neurocognitive and social-behavioral abnormalities. Recent studies of murine knockouts of key genes in the microdeleted region, LIM kinase 1 (LIMK1) and cytoplasmatic linker protein 2 (CYLN2), demonstrated significant functional and metabolic abnormalities, but grossly normal structure, in the hippocampal formation (HF). Furthermore, deficits in spatial navigation and long-term memory, major cognitive domains dependent on hippocampal function, have been described in WS. We used multimodal neuroimaging to characterize hippocampal structure, function, and metabolic integrity in 12 participants with WS and 12 age-, sex-, and IQ-matched healthy controls. PET and functional MRI studies showed profound reduction in resting blood flow and absent differential response to visual stimuli in the anterior HF in WS. Spectroscopic measures of N-acetyl aspartate, considered a marker of synaptic activity, were reduced. Hippocampal size was preserved, but subtle alterations in shape were present. These data demonstrate abnormalities in HF in WS in agreement with murine models, implicate LIMK1 and CYLN2 in human hippocampal function, and suggest that hippocampal dysfunction may contribute to neurocognitive abnormalities in WS.
Subject(s)
Hippocampus/pathology , Hippocampus/physiopathology , Williams Syndrome/pathology , Williams Syndrome/physiopathology , Adult , Animals , Aspartic Acid/analogs & derivatives , Aspartic Acid/metabolism , Case-Control Studies , DNA-Binding Proteins/deficiency , DNA-Binding Proteins/genetics , Female , Hippocampus/blood supply , Humans , Lim Kinases , Magnetic Resonance Imaging , Male , Mice , Mice, Knockout , Microtubule-Associated Proteins/deficiency , Microtubule-Associated Proteins/genetics , Nerve Tissue Proteins/deficiency , Nerve Tissue Proteins/genetics , Photic Stimulation , Positron-Emission Tomography , Protein Kinases/deficiency , Protein Kinases/genetics , Protein Serine-Threonine Kinases/deficiency , Protein Serine-Threonine Kinases/genetics , Williams Syndrome/geneticsABSTRACT
Using multimodal neuroimaging in humans, we demonstrate specific interactions between prefrontal activity and midbrain dopaminergic synthesis. A common V(108/158)M substitution in the gene for catecholamine-O-methyltransferase (COMT), an important enzyme regulating prefrontal dopamine turnover, predicted reduced dopamine synthesis in midbrain and qualitatively affected the interaction with prefrontal cortex. These data implicate a dopaminergic tuning mechanism in prefrontal cortex and suggest a systems-level mechanism for cognitive and neuropsychiatric associations with COMT.
