1.
Org Lett
; 2(14): 2027-30, 2000 Jul 13.
Article
in English
| MEDLINE
| ID: mdl-10891221
ABSTRACT
[reaction: see text] We present an irreversible and efficient protease-based method for peptide synthesis which occurs independently of the primary specificity of proteases and also without proteolytic side reactions. The key feature of this approach is the combination of the substrate mimetics strategy with frozen state enzymology. Model reactions catalyzed by several proteases qualify this approach as a powerful concept in the direction of a more universal application of proteases as biocatalysts for peptide ligation.