ABSTRACT
The detection of antibodies to Core-antigen of virus of hepatitis C in test-systems for solid-phase immune-enzyme analysis with low optical density can be a result not only of true availability of antibodies but an effect of nonspecific reaction of blood serum. The diagnostic possibilities of immunochips to be used in immune-enzyme analysis for verification of availability of markers of viral hepatitis C were investigated in conditions of low positive reaction of blood serum to core-antigen. It is established that immunochips and immunoblots have similar specificity concerning detection of antibodies to Core-antigen. At that, in immunochips antibodies to nonstructural antigens of virus of hepatitis C were additionally detected in more than 90% of samples.
Subject(s)
Hepacivirus/isolation & purification , Hepatitis C Antibodies , Hepatitis C/diagnosis , Viral Core Proteins/isolation & purification , Adolescent , Adult , Aged , Female , Hepacivirus/pathogenicity , Hepatitis C/blood , Hepatitis C/virology , Hepatitis C Antibodies/blood , Hepatitis C Antibodies/immunology , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Male , Middle Aged , Pregnancy , Viral Core Proteins/bloodABSTRACT
The new kit of reagents in format of the immunochip "ImmunoChip Borreliosis" for multiplex serologic analysis of mite-borne borreliosis demonstrated high diagnostic sensitivity and specificity. The percentage of detection of specific immunoglobulins was higher in "ImmunoChip Borreliosis" as compared with screening results in immune enzyme analysis. The high correlation between results of testing in immunochip and data of immune blotting is demonstrated to.
