[The results of studies of the peripheral blood from children in Polesie District, Kiev Province and in the city of Kiev 4 years after the accident at the Chernobyl Atomic Electric Power Station]. / Rezul'taty issledovanii perifericheskoi krovi detei Polesskogo raiona Kievskoi oblasti i g. Kieva cherez chetyre goda posle avarii na Chernobyl'skoi AES.

The peripheral blood of 2200 children (age: from 2 to 16 years) inhabiting the Polessye District of Kiev Province and the city of Kiev 4 years after the Chernobyl atomic station accident was studied. An analysis of results revealed the presence of hematologic changes that may be interpreted as sequels of the effect of ionizing radiation on the body, while other changes may be considered as reactive and may in most cases accompany different somatic diseases of infectious-inflammatory character.

[A karyotype and chromatin study of the peripheral blood lymphocytes of patients with pigmented skin neoplasms]. / Issledovanie kariotipa i khromatina limfotsitov perifericheskoi krovi bol'nykh pigmentnymi novoobrazovaniiami kozhi.

Rearrangements of genetic material in the peripheral blood lymphocyte nuclei have been found in patients with dysplastic pigmented nevi and malignant melanomas (15 cases), in contrast to the control (10 healthy persons). It is expressed as an increase in the number of chromosome aberrations in the karyotype and as a change of the Feulgen-positive substance correlation in the interphase nuclei of the given cells towards an increase in the active-functioning DNA structures.

[Cryopreservation of a suspension of fetal liver cells for clinical use]. / Kriokonservirovanie suspenzii kletok fetal'noi pecheni dlia klinicheskogo primeneniia.

The authors evaluate a method of procuring and preserving fetal liver cells in a medium containing low-molecular polyvynilpyrrolidon, glucose, lactose, sodium phosphate and sodiumhydrocitrate not requiring washing off. The biomaterial is stored at a temperature of -196 degrees C. The efficiency of this method of cryopreservation of fetal liver cell suspensions was confirmed by high morphological intactness, functional activity and proliferative capacity. Patients with hypoplastic anemia subjected to transfusion of cryopreserved fetal liver cells showed a normalization of bone marrow hemopoiesis, remissions.

Electrophoretic marker of differentiated human prostate secretory epithelial cells.

Electrophoretic analysis of primordial human prostate epithelium, glandular secretory epithelium of prostate and other human tissues revealed a marker characteristic of differentiated secretory prostate cells. Being probably neither protein nor lipid in chemical nature, this marker reacted in a peculiar way with Amido Black 10B. The marker was shown to be present in prostatic secretory substance.

[In vitro models for research on cell differentiation and malignant degeneration in the human prostate]. / Modeli in vitro dlia izucheniia differentisirovki i malignizatsii kletok predstatel'noi zhelezy cheloveka.

The methods are reviewed for obtaining monolayer epithelium cultures both in normal and hyperplastic or malignant prostate glands. Preparation of pure epithelial tissue cultures is dealt with in detail. The major prostate cell lines are described. Special attention is paid to the markers of differentiation and sensitivity to hormones in normal and tumour prostatic cells in vitro. Prospects for the use of prostatic cell cultures as models in oncology are outlined.

[Colony-forming ability of bone marrow in children with acute leukemia]. / Kolonieobrazuiushchaia sposobnost' kostnogo mozga pri ostrom leikoze u detei.

Colony- and cluster-forming ability of bone marrow cells in agar medium was studied in 6 healthy children and 13 children suffering from acute leukosis. During acute stages of the diseases colonies and clusters in agar medium consisted of granulocytic cells and their quantity depended on the safety of predecessors of bone marrow granulopoiesis. In contrast to acute lymphoblastic leucosis, acute myeloblastic leucosis is characterized by a sharp growth of cluster-forming and a less intensive growth of colony-forming ability of hemopoietic cells. The bone marrow remission is marked by the growth of diffusive and mixed colonies and clusters, consisting of granulomonocytic elements. The low level of colony- and cluster-formation in the medium at the last stage of therapy (induction of acute leukosis remission) correlated well with positive indices of treatment effectiveness. The results obtained during the cultivation of blood-forming cells in the agar medium may be also used for diagnosis and prognosis.

[Comparative study of the karyotype and histostructure of neoplasms of the uterine body, stomach and malignant melanoma]. / Sravnitel'noe izuchenie kariotipa i gistostruktury novoobrazovanii tela matki, zheludka i zlokachestvennoi melanomy.

Direct analysis of karyotype revealed a heterogeneity of chromosome composition, variations in modal lines and chromosome aberrations, diversity of marker chromosome morphology. The variability of cytogenetic alterations was found in tumors both of the same and of different origin. These results established the nature of genome and chromosome alterations in the investigated tumors. The problem is discussed of a possible use of cytogenetic analysis for a more concrete characterization of human tumors in terms of their biological features.