[Development of the immunoenzyme test-system for detection of Ebola virus antigen]. / Razrabotka immunofermentnoi test-sistemy dlia opredeleniia antigena virusa Ebola.

An enzyme immunoassay system has been developed for the detection of Ebola virus antigen. It permits a highly accurate and sensitive rapid detection of the antigen. Optimal dilutions of specific immunoglobulin (1:500, corresponding to protein concentration of 50 micrograms/ml) and conjugate were found. The resolving capacity of the new test system is 1.9 x 10(-7) g protein.

[A method of fluorescent immunoenzyme analysis in the complex evaluation of viral and rickettsial vaccines]. / Metod immunofermentnogo fliuorestsentnogo analiza v kompleksnoi otsenke virusnykh i rikketsioznykh vaktsin.

With the use of the unified indirect solid-phase fluorescent enzyme immunoassay the combined evaluation of the antigenic and immunogenic properties of experimental whole-virion inactivated virus vaccines against Venezuelan and eastern equine encephalomyelitides, as well as of dried chemical typhus vaccine, has been made; their safety was determined indirectly by the content of ovalbumin. The protective role of antibodies evaluated by the solid-phase fluorescent enzyme immunoassay in typhus immunity has been shown.

[A comparative evaluation of an immunoenzyme analytical method using a fluorogenic or chromogenic substrate in determining the Marburg virus antigen]. / Sravnitel'naia otsenka metoda immunofermentnogo analiza s primeneniem fliuorogennogo ili khromogennogo substrata pri opredelenii antigena virusa Marburg.

A comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of Marburg virus antigen was carried out. The resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-8) g of protein for the latter substrate. Cross titrations demonstrated the lack of common antigens with Ebola virus.

[Immunoenzyme analysis in a system of serologic evaluation of louse-borne typhus vaccines]. / Immunofermentnyi analiz v sisteme serologicheskoi otsenki sypnotifoznykh vaktsin.

The blood sera of persons immunized with different typhus vaccines have been studied in the complement fixation test, the indirect hemagglutination test and the enzyme immunoassay. The data thus obtained indicate that the enzyme immunoassay is highly sensitive and can be universally used for the determination of antibodies to Rickettsia prowazekii after primary and booster immunization with different typhus vaccines. This method detects specific antibodies both at an early period and, which is of particular importance, at a remote period after immunization (3 years later) when complement-binding and hemagglutinating antibodies are absent. This is seemingly indicative of the two-phase character of postvaccinal immunity induced by live typhus vaccine.