Adhesion, growth, and proliferation of endothelial cells on biopolymer extracellular film matrices.

We analyzed the influence of initial properties of biopolymer fi lm extracellular matrices made of chitosan on adhesion of swine embryonic kidney epithelium-like cells, their morphology, growth, and proliferation. It was found that adhesion index of cells on the studied matrices was similar, but cell morphology had significant qualitative differences. Round non-flattened cells were described in endothelial cell cultures on chitosan-based matrices. Local variations in charge density on matrices induced by the presence of chitin nanofibers led to the appearance of flattened spindle-shaped and non-flattened sphericalal cells. Long-term culturing on biopolymer extracellular fi lm matrices was associated by the formation of spatial conglomerates of sphericalal cells.

[Dynamics of structural change of ovarian tissue under ischemia].

In this work it was investigated the dependence of the correlation of folliculogenesis and endocrine function of ovarian tissue on the degree of structural damage and oocyte volume changes during ischemia. It was shown that after 2 hours of ischemia at 37 °C the morphological transformation of the structural components of the ovarian tissue were reversible. In case of restoration of blood flow conditions by heterotopic transplantation estradiol level of animals was 25,9 ± 5,18 pg/ml, progesterone--18,48 ± 3,69 ng/ml, significantly higher than the castrated animals-recipients. Supplement of the incubation medium by the fetal bovine serum lead to decreasing by 5-7% in the volume of oocytes of growing follicles during in ischemia and reduced steroidogenic function of ovarian tissue after perfusion. Increased time of the ischemia up to 4 hours was founded in irreversible morphological transformation, reduce the volume of oocytes by 40% and the formation of sclerosed tissue after transplantation of the ischemic fragments of ovarian tissue.

[Dynamics of morphological and functional development of ovarian tissue allografts].

We examined the dynamics of folliculogenesis in ovarian tissue allograft and its endocrine function in an organism of castrated recipient rats during 100 days. Growth and development of ovarian tissue and its steroidogenic function in heterotopic transplantation were determined by its initial morphological organization. Allotransplantation of mature ovarian tissue was found to cause preservation of its morphological structure, whereas the transplantation of neonatal ovarian tissue caused formation of follicular cysts. At 100th day of observation, we observed a recovery of the levels of sex hormones to the physiological norm after transplantation of both mature ovarian tissue and neonatal tissue of 10-days of postnatal development.

[Dynamics of folliculogenesis of sexually mature and neonatal ovarian tissue under conditions of long-term heterotopical transplantation].

In this work, a comparative analysis of dynamics of morphological development and endocrine function of transplants of sexually mature and neonatal ovarian tissue was performed under conditions of bilateral ovariectomy of animals-recipients. It has been established that at transplantation of the sexually mature ovarian tissue there were observed all stages of folliculogenesis and preservation of endocrine function at long-term observation (up to 100 days). At transplantation of neonatal ovarian tissue there was revealed a pathological picture of its development: hyperplasia of stromal structure and formation of cysts and cystomae. Follicles of different degree of maturity were revealed in 30 % of animals at the 30th day of observation. A significant increase of concentration of sex hormones as compared with ovariectomied animals has been shown only at early stages after implantation (30 days).

[The effect of initial hormonal status of recipient animals on morphological state and endocrine function of ovarian tissue transplant].

The aim of investigation was a comparative analysis of morphofunctional characteristics of allografts of mature ovarian tissue and neonatal ovary depending on initial hormonal status of recipient animals. Rats' mature ovarian tissue and neonatal ovary were transplanted under renal capsule simultaneously with ovariectomy and 2 months later. Data analysis indicated that the development of ovarian tissue transplant was determined by its maturity and initial hormonal status of recipient. Mature ovarian tissue transplants preserve morphology and endocrine function to the 60th day after transplantation to the recipient animals with the initial hormonal status that corresponds to the norm. Neonatal ovary transplants are developed to the mature stage following transplantation to recipients with hormonal status that corresponded to the ovariectomized animals, that is, low concentration of sex hormones and an increased level of follicle-stimulating hormone.