ABSTRACT
MicroRNA-21 (miR-21) expression was quantified by real-time qRT-PCR in peripheral blood and saliva samples obtained from patients diagnosed with colorectal cancer (CRC) of varying degrees of malignancy and healthy volunteers. All patients had adenocarcinoma located in the distal colon at different stages. Significant differences were detected between the control group and the total experimental group of CRC patients (plasma, P = 0.0001; saliva, P = 5e-12). MiR-21 expression was also significantly different in certain subgroups of patients with CRC disease stages II-IV as compared to the control group. No correlation of miR-21 expression was found with regard to gender and age of patents. Also, there were no significant individual correlations and linear regression of miR-21 expression in the plasma and saliva. The estimated diagnostic sensitivity and specificity of miR-21 expression were respectively 65 and 85% in the plasma, and 97 and 91% in the saliva. Our data suggest that miR-21 in both the saliva and plasma could be a proper biomarker for CRC screening, although the saliva miR-21 expression test looks preferable due to its higher sensitivity, specificity, and technical simplicity.
Subject(s)
Colorectal Neoplasms/genetics , MicroRNAs/genetics , Saliva/chemistry , Aged , Aged, 80 and over , Biomarkers, Tumor/analysis , Biomarkers, Tumor/blood , Biomarkers, Tumor/genetics , Case-Control Studies , Female , Humans , Male , MicroRNAs/analysis , MicroRNAs/blood , Middle Aged , Neoplasm Staging , Sensitivity and SpecificityABSTRACT
The ultrastructure of myofibrillar and mitochondrial apparatus of m. soleus and m. vastus lateralis of Bion 11 monkeys was investigated. In both muscles, the volume density and the mean area of myofibrils increased insignificantly and their number decreased; in m. soleus the mean area of mitochondria increased significantly and in m. vastus lateralis insignificantly.
Subject(s)
Mitochondria, Muscle/ultrastructure , Muscle, Skeletal/ultrastructure , Myofibrils/ultrastructure , Space Flight , Weightlessness , Adaptation, Physiological , Animals , Macaca mulatta , Male , Microscopy, Electron , Muscle Fibers, Skeletal/ultrastructureABSTRACT
Electron microscopical studies of the nucleoid structure of hydrogen bacteria using ultrahin sections and spread DNA from bacterial cell lysates revealed a different DNA packaging in the cell. A compact state of the major part of DNA at all growth stages and stability of nucleosome-like structures were shown. The use of antibodies to HU protein of E. coli labelled by protein A-colloidal gold demonstrated the immunological relationship between HU protein of E. coli and histone-like proteins of Alcaligenes eutrophus and their possible role in the nucleosome-like DNA packaging in procariotic genome.