ABSTRACT
The changes of locomotor activities in rat loaded with swimming exercise were recorded by our newly devised apparatus. In addition, changes of lipid peroxide levels and their related enzyme activities in rat brain, liver as well as blood were studied. The results obtained were as follows: 1. The locomotor activities in rat recorded by the apparatus showed the same patterns as that reported by the other researchers. 2. After the loading of swimming, locomotor activities in rat during the dark period decreased significantly as compared to those of the control. 3. The levels of TBARS (thiobarbituric acid reactive substance), SOD (superoxide dismutase) and GSH-px (glutathione peroxidase) in rat liver elevated after the swimming exercise in the first group, which was sacrificed after loading with one treatment (about 5 hours) exercise of swimming. 4. The level of TBARS in rat brain elevated after the swimming exercise in the second group, which was sacrificed after loading with two treatment exercise of swimming. 5. The level of TBARS in plasma decreased, and GSH-px, GR (glutathione reductase) and catalase in red blood cells elevated in the third group, which was sacrificed after two-hour rest following the loading with two treatment exercise of swimming. It is indicated that our newly devised apparatus is useful for monitoring locomotor activities in rat, and that the fatigue in rat caused by swimming load can be shown in terms of changes in the above activities. The elevation of the level of TBARS during the swimming exercise observed in tissues of the brain and liver may suggest that the lipid peroxidation will reflect a certain state of fatigue in rat.
Subject(s)
Lipid Peroxides/metabolism , Movement , Physical Exertion , Animals , Brain Chemistry , Circadian Rhythm , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Male , Rats , Superoxide Dismutase/metabolism , Swimming , Thiobarbiturates/immunology , Tissue DistributionABSTRACT
The authors have devised an apparatus for monitoring the spontaneous locomotor activity of rats, with which the patterns of locomotor activity under the condition of light-dark cycle (light period: twelve hours from 6 a.m., dark period: the other half of the day) were analysed. In addition, changes in locomotor activities due to forced exercise (swimming) were recorded. The results obtained were as follows: 1. The patterns of locomotor activities in rats recorded by the monitor showed the same patterns as reported already by other researchers. The authors believe that this new apparatus can be used practically as a locomotor activity monitor. 2. Due to exhaustive exercise by swimming, changes of the ultradian rhythm in rats were observed. 3. By loading of swimming, locomotor activities in rats decreased significantly, especially in the dark period. These results suggest that this newly devised apparatus is useful as a locomotor activity monitor, and that the fatigue in rats caused by physical load can be shown as the change in locomotor activities.
Subject(s)
Locomotion , Physical Exertion , Animals , Circadian Rhythm , Fatigue , Male , RatsABSTRACT
1,215 male workers using bush cleaners in Hokkaido were examined by the method based on the notification of the Labor Standard Bureau. The results obtained from clinically normal 548 workers among them were analysed especially on the finger skin temperature, vibratory sense threshold, maximum grip strength. 1) Data of the vibratory sense threshold, maximum grip strength and endurable grip strength showed normal distributions. Data of the finger skin temperature, however, showed a skewed distribution. 2) In parallel with aging, the vibratory sense threshold increased, in other words, lowered in function, and the maximum grip strength lowered. 3) The endurable grip strength measured by the five-time repetition method decreased along with aging. 4) Values of one-tail limit of the finger skin temperature were calculated in all 548 workers, and those of the vibratory sense threshold, maximum grip strength and endurable grip strength were done in each age group. The values were compared with the already proposed criteria and discussed.
Subject(s)
Occupational Diseases/diagnosis , Vibration/adverse effects , Adult , Aged , Humans , Male , Middle Aged , Raynaud Disease/diagnosis , Sensory Thresholds , Skin TemperatureSubject(s)
Acidosis, Renal Tubular/urine , Beta-Globulins/urine , Carbonic Anhydrases/urine , Metals/toxicity , Adult , Aged , Cadmium/toxicity , Cadmium/urine , Child , Child, Preschool , Environmental Exposure , Female , Humans , Lead/toxicity , Lead/urine , Male , Mercury/toxicity , Mercury/urine , Metals/blood , Metals/urine , Middle Aged , RadioimmunoassayABSTRACT
Untrained healthy male volunteers were subjected to the study on the effects of exercise (bicycle ergometer, 920 kpm/min for 30 min) upon GOT isozyme (supernatant GOT, s-GOT and mitochondrial GOT, m-GOT) activities in plasma. Significant increases in the levels of both s-GOT and m-GOT activity were observed after the exercise (p less than 0.001). After 30 min of rest m-GOT activity almost returned to its preexercise value. However, s-GOT activity did not return to its preexercise activity. The changes in the activity of GOT isozyme after physical exercise are discussed.
