ABSTRACT
BACKGROUND/OBJECTIVE: Genetic polymorphisms in cytochrome P-450 (CYPs) and glutathione S-transferase (GSTs) genes can influence the appearance of tumors by the formation of new enzymes with altered activities. In the present study, 5 polymorphic variants were examined in 154 patients with prostate carcinoma and in 154 controls. MATERIALS AND METHODS: DNA analysis was carried out through PCR-based methods. The statistical methods used were odds ratio and confidence interval (95% CI), χ(2), Fisher, and Mann-Whitney. RESULTS: The study showed absence of association for CYP1A1 2B, CYP1B1 2, GSTM1 0, and GSTT1 0. The statistical analysis implied a positive association of variant CYP3A4 1B for prostate cancer. The combined analysis of CYP1A1 2B, CYP1B1 2, and CYP3A4 1B genotypes showed positive association. The analysis of histopathologic parameters detected statistically significant differences for Gleason score and biochemistry recurrence risk. The presence of the GSTT1 0 genotype in red meat consumers increased the risk for this disease. CONCLUSION: Some polymorphic variants analyzed can influence the development and the progression of prostate cancer.
Subject(s)
Aryl Hydrocarbon Hydroxylases/genetics , Cytochrome P-450 CYP1A1/genetics , Cytochrome P-450 CYP3A/genetics , Glutathione Transferase/genetics , Prostatic Neoplasms/genetics , Adult , Aged , Aged, 80 and over , Case-Control Studies , Cytochrome P-450 CYP1B1 , Genetic Predisposition to Disease , Genotype , Humans , Male , Middle Aged , Polymerase Chain Reaction , Polymorphism, Single Nucleotide , Prostatic Neoplasms/enzymologyABSTRACT
The study of genes involved in androgen pathway can contribute to a better knowledge of prostate cancer. Our aim was to examine if polymorphisms in prostate-specific antigen (PSA) and androgen receptor (AR) genes were involved in prostate cancer risk and aggressiveness. Genotypes were determined by PCR-RFLP (PSA) or using a 377 ABI DNA Sequencer (AR). PSA(G/G) genotype (OR = 1.78, 95% CI = 1.062.99) and AR short CAG repeats (OR = 1.89, 95% CI = 1.212.96) increased risk for prostate cancer and were related with tumor aggressiveness. About 38.3% of tumors showed microsatellite instability. In conclusion, polymorphisms in these genes may be indicated as potential biomarkers for prostate cancer.
Subject(s)
Polymorphism, Genetic , Prostate-Specific Antigen/genetics , Prostatic Neoplasms/genetics , Receptors, Androgen/genetics , Aged , Alleles , Biomarkers, Tumor/analysis , Biomarkers, Tumor/genetics , Gene Frequency , Genetic Predisposition to Disease/genetics , Genotype , Humans , Male , Middle Aged , Neoplasm Invasiveness , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Prostatic Neoplasms/pathology , Sequence Analysis, DNA , Trinucleotide Repeats/geneticsABSTRACT
O objetivo deste trabalho é verificar a capacidade indutora de matriz óssea desmineralizada em falhas no rádio de coelhos, fixadas por pino intramedualr. Os animais foram divididos em 3 grupos: Grupo 1 - a falha foi fixada com pino intramedular; Grupo 2 - a falha foi fixada com pino intramedular e preenchida com matriz óssea desmineralizada; Grupo 3- a falha foi somente preenchida com matriz óssea desmineralizada. Foi feito acompanhamento radiológico a intervalos de 3 semanas, durante 12 semanas. As avaliaç 8es macroscópicas e histológicas foram feitas na 12ª semana. Os resultados radiológicos no grupo 1, na 3ª semana mostram falha óssea radiotransparente, enquanto que 12ª semana há imagem radiopaca que preenche parcialmente a falha. No grupo 2, há pequena imagem radiopaca central na 3ª semana, que aumenta consideravelmente na 12ª semana. No grupo 3, a imagem radiopaca, na 3ª semana é evidente no centro da falha, e há preenchimento toral na 12ª semana. Macroscopicamente e histologicamente a intensidade de neoformaçäo óssea corresponde a evoluçäo radiológica nos 3 grupos estudados. Os resultados mostram que houve retardo no preenchimento da falha óssea, nos grupos com fixaçäo. Isso nos leva à deduzir que a utilizaçäo do PIM facilita a fixaçäo, no entanto, retarda a neoformaçäo óssea por levar à alteraçöes circulatórias, dado a destruiçäo de parte da medula óssea
