ABSTRACT
Some indices have been studied which characterized the state of Epstein-Barr virus genome and adenovirus in the implanted lines of lymphoblastoid cells of B and T phenotype under the mixed or monoinfection. It has been shown that super infection by type 2 adenovirus rather sharply affects the state of Epstein-Barr virus genome in the Raji cells containing integrated Epstein-Barr virus genome. The state of adenovirus genome in the studied cells is less subject to changes. Its early area is revealed by hybridization using DNA-DNA method in a form of two fragments of different intensity which is maximum in the Raji and Jurkat cells, which evidences for the more expressivity of adenovirus genome in these cells.
Subject(s)
Adenovirus Infections, Human/genetics , Adenoviruses, Human/genetics , B-Lymphocytes/virology , Genome, Viral , Herpesviridae Infections/genetics , Herpesvirus 4, Human/genetics , T-Lymphocytes/virology , Tumor Virus Infections/genetics , Adenovirus Infections, Human/virology , Animals , Callithrix , Cell Line , Cells, Cultured , DNA, Viral/genetics , Herpesviridae Infections/virology , Humans , Nucleic Acid Hybridization/methods , Polymerase Chain Reaction/methods , Tumor Virus Infections/virology , Virus CultivationABSTRACT
The results of molecular investigations of blood mononuclears from 120 clean-up workers after 7-9 years of Chernobyl accident with the total exposure radiation doses ranging from 5 to 76 cGr are presented. Structural polymorphism of the leukemia associated bcr and ribosomal RNA (rRNA) genes were studied using Southern blot hybridization. Allelic polymorphism of bcr gene with characteristic for leukemia allele distribution was detected in 16.6%. Rearrangements of rRNA genes were observed in 13% of Chernobyl accident clean-up workers.
Subject(s)
Genome, Human , Leukocytes, Mononuclear/radiation effects , Power Plants , Radioactive Hazard Release , Alleles , DNA/blood , DNA/radiation effects , Dose-Response Relationship, Radiation , Fusion Proteins, bcr-abl/genetics , Fusion Proteins, bcr-abl/radiation effects , Humans , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics , Leukocytes, Mononuclear/ultrastructure , Microscopy, Electron , Polymorphism, Genetic/radiation effects , RNA, Ribosomal/genetics , RNA, Ribosomal/radiation effects , Time Factors , UkraineABSTRACT
Expression of genes, associated with leukemic transformation and called x-mla, was studied in cells of various types of experimental leukemia and in homologous normal cells. Augmentation of x-mla expression in malignant haemopoietic cells and lack or very low expression in normal tissues and non-haemopoietic tumors of mice and rats has been revealed. The involving of x-mla in proliferation control of leukemic but not normal cells has been shown. Product of x-mla--p46 was synthesized in cell-free translational system and purified. Its amino acid composition was determined and its mitogenic, RNAase activities, and ability to phosphorylation have been investigated.
Subject(s)
Cell Transformation, Neoplastic/genetics , Gene Expression Regulation, Neoplastic/physiology , Leukemia, Experimental/genetics , Amino Acids/analysis , Animals , Cell Division/genetics , Cell-Free System , Mice , Mice, Inbred Strains , Neoplasm Proteins/biosynthesis , Phosphorylation , Protein Biosynthesis , Rats , Rats, Wistar , Reference Values , Ribonucleases/metabolismABSTRACT
The effects of the erythrocyte-specific histone H5 from chicken erythrocytes on HnRNA synthesis in splenocytes of Rauscher virus-infected mice were studied. It was shown that histone H5 represses the synthesis of 35S RNA typical of the Rauscher virus-producing cells but does not influence the synthesis of other HnRNA fractions. The effects of H5 and other histones on the effectiveness of poly(U) translation in a cell-free system of wheat embryos were investigated. Structurally similar histones H1 and H5 exert the strongest inhibiting action on translation. It was assumed that the previously described antileukemic action of histone H5 is realized at the levels of transcription and translation.
Subject(s)
Histones/pharmacology , Leukemia, Experimental/metabolism , Protein Biosynthesis/drug effects , RNA, Neoplasm/biosynthesis , Animals , Chickens , Erythrocytes/metabolism , In Vitro Techniques , Mice , Mice, Inbred BALB C , RNA, Transfer/biosynthesis , Rauscher VirusABSTRACT
Development of virus-induced Rauscher leukosis was studied after administration into mice of histone H5 isolated from chicken erythrocyte nuclei by means of gel filtration through Sephadex G-75. Histone H5, administered intraperitoneally at a dose of 100 mg/kg into mice treated with Rauscher virus, inhibited the leukosis development (T = 29.7%). Inhibitory effect of histone H5 on synthesis of 35S RNA, characteristic for leukemic cells, was found. The most distinct efficiency of the histone was exhibited within early periods of the infection in experimental animals.
Subject(s)
Histones/pharmacology , Leukemia, Experimental/metabolism , RNA, Neoplasm/biosynthesis , Animals , Chickens , Electrophoresis, Disc , Erythrocytes/analysis , Histones/isolation & purification , Mice , Mice, Inbred BALB C , Rauscher Virus , Spleen/metabolismABSTRACT
A component of quickly labelling D-RNA with a sedimentation coefficient of 35 S is revealed in spleen cell nuclei of mice infected with the leukemogenic Rauscher virus. The component is absent in healthy animals. It is labelled for 5 min of incubation with the label and under conditions of chase experiment disappears from the nucleus 2 h later differing from the rest of leukemic cell D-RNA in a higher metabolic activity. [3H] RNA with a sedimentation coefficient of 35 S is detected in cytoplasm of RNA synthetizing in leukemic cells under the effect of leukemogenic virus. It is the RNA of messenger type though it seems possible that a part of it remains in the nucleus and performs intranuclear functions.
