ABSTRACT
A new method has been developed for the rapid analysis of psilocybin and/or psilocin in fungus material using ion mobility spectrometry. Quantitative analysis was performed by gas chromatography-mass spectrometry after a simple one-step extraction involving homogenization of the dried fruit bodies of fungi in chloroform and derivatization with MSTFA. The proposed methods resulted in rapid procedures useful in analyzing psychotropic fungi for psilocybin and psilocin.
Subject(s)
Agaricales/chemistry , Gas Chromatography-Mass Spectrometry/methods , Hallucinogens/analysis , Mass Spectrometry/methods , Psilocybin/analogs & derivatives , Psilocybin/analysis , Drug and Narcotic Control , Forensic Medicine , Humans , Mass Spectrometry/instrumentation , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
A suicidal ingestion of an unknown quantity of Resochin (chloroquine) tablets is described. Although chloroquine is known since 1934, intoxications due to chloroquine overdose are rather rare in European countries. The authors report on a new and fast method of analysing and determining the chloroquine concentration in body fluids and postmortem specimens. The analytes were extracted from alkalinized samples into ethyl acetate before GC/MS analysis. The analyses of chloroquine were performed without any complex sample clean-up steps and, in addition, with little sample material. The proposed method resulted in a rapid procedure most useful in cases of deliberate poisoning with the anti-inflammatory and antimalarial drug chloroquine.
Subject(s)
Antimalarials/poisoning , Chloroquine/analogs & derivatives , Suicide , Adult , Antimalarials/blood , Antimalarials/pharmacokinetics , Chloroquine/blood , Chloroquine/pharmacokinetics , Chloroquine/poisoning , Fatal Outcome , Gas Chromatography-Mass Spectrometry , Humans , Intestinal Absorption , Male , Tissue DistributionABSTRACT
A case of lethal cocaine poisoning after parenteral application is described. The results of the quantitative gas chromatographic determination in blood, liver, urine, stomach contents and muscle are reported.
Subject(s)
Cocaine/poisoning , Forensic Medicine , Suicide/legislation & jurisprudence , Adult , Cocaine/metabolism , Humans , Male , Tissue DistributionSubject(s)
Blood Preservation , Ethanol/blood , Fluorides/pharmacology , Ethanol/metabolism , Humans , Oxidation-Reduction , Time FactorsABSTRACT
After a short description of sources of lead poisoning a review is given of toxicity, absorption, storage and excretion of lead. Afterwards its biochemical affects--especially the influence on the biosynthesis of heme--are treated. Methods for diagnosis of lead poisoning resulting from these affects--determination of coproporphyrine and delta-aminolevulinic acid excretion in urine, erythrocyte delta-aminolevulinic acid dehydratase activity and erythrocyte protoporphyrin--are discussed in detail.
Subject(s)
Lead Poisoning/diagnosis , Aminolevulinic Acid/urine , Coproporphyrins/urine , Economics , Erythrocytes/enzymology , Erythrocytes/metabolism , Heme/biosynthesis , Humans , Lead/adverse effects , Lead/metabolism , Lead/pharmacology , Lead Poisoning/metabolism , Methods , Occupational Diseases/chemically induced , Porphobilinogen Synthase/blood , Protoporphyrins/blood , SuicideSubject(s)
Asphyxia/pathology , Pulmonary Edema/pathology , Animals , Asphyxia/etiology , Drowning , Lung/pathology , Pulmonary Edema/etiology , RatsABSTRACT
The determination of lead in blood, urine and bone (0.5 to 1.0 g blood, 5 to 10 ml urine, approx. 200 mg bone ash) by anodic stripping voltammetry is described. Blood samples are decomposed with nitric acid/perchloric acid, urine samples with nitric acid/perchloric acid/sulfuric acid. Bones are dry-ashed at 450 to 500 degrees C previously. The interference of iron in the analysis of blood samples is removed by adding citrate and adjusting the pH to approx. 3.5.
