ABSTRACT
Three nutrients, iron, zinc and pro-vitamin A, are widely deficient in humans, especially among low socioeconomic groups in developing countries, but they remain significant concerns in industrialized countries as well. Cereals provide the majority of the intake of these nutrients in low-income families. Moreover, these three nutrients may interact synergistically in absorption and function to such an extent that there are potentially huge advantages in providing all three together in the one staple food. Because of this, they may be more bioavailable to deficient individuals than current thinking allows. To do so would provide a sound basis on which to build a better balanced diet for nutritionally compromised individuals. Genetic variation in nutrient composition exists in cereals and can be exploited in conventional breeding programmes and through gene technology. Cultural techniques, including fertiliser technology and organic farming, have also impacted upon the nutrient composition of cereals. Human iron and zinc intake can be doubled at least, and essential carotenoid intakes can be increased dramatically. Preliminary feeding trials with nutrient-dense grains have been encouraging. Moreover, nutrient-dense seeds also produce more vigorous seedlings and higher grain yield in soils where these nutrients are poorly available, so that to a significant extent agronomic and health objectives coincide. New varieties are rapidly adopted, especially where there are yield advantages, ensuring maximum impact without new inputs. This approach is potentially more sustainable than fortification and supplementation programmes because intake is continuous, which is especially important for zinc because it is needed almost daily.
ABSTRACT
The initiation of Escherichia coli chromosomal replication by DnaA protein is strongly influenced by the tight binding of the nucleotides ATP and ADP. Anionic phospholipids in a fluid bilayer promote the conversion of inactive ADP-DnaA protein to replicatively active ATP-DnaA protein in vitro, and thus likely play a key role in regulating DnaA activity. Previous studies have revealed that, during this reactivation, a specific region of DnaA protein inserts into the hydrophobic portion of the lipid bilayer in an acidic phospholipid-dependent manner. To elucidate the requirement for acidic phospholipids in the reactivation process, the contribution of electrostatic forces in the interaction of DnaA and lipid was examined. DnaA-lipid binding required anionic phospholipids, and DnaA-lipid binding as well as lipid-mediated release of DnaA-bound nucleotide were inhibited by increased ionic strength, suggesting the involvement of electrostatic interactions in these processes. As the vesicular content of acidic phospholipids was increased, both nucleotide release and DnaA-lipid binding increased in a linear, parallel manner. Given that DnaA-membrane binding, the insertion of DnaA into the membrane, and the consequent nucleotide release all require anionic phospholipids, the acidic headgroup may be necessary to recruit DnaA protein to the membrane for insertion and subsequent reactivation for replication.
