ABSTRACT
The newly discovered HLA-C*04:01:01:186 allele differs from HLA-C*04:01:01:01 by a single nucleotide substitution in intron 3.
Subject(s)
Alleles , HLA-C Antigens , High-Throughput Nucleotide Sequencing , Histocompatibility Testing , Introns , Humans , HLA-C Antigens/genetics , High-Throughput Nucleotide Sequencing/methods , Histocompatibility Testing/methods , Greece , Polymorphism, Single Nucleotide , Exons , Base Sequence , Sequence Analysis, DNA/methodsABSTRACT
HLA-B*38:01:01:18 differs from the HLA-B*38:01:01:01 allele by one nucleotide substitution in the 5'UTR.
Subject(s)
5' Untranslated Regions , Alleles , HLA-B Antigens , High-Throughput Nucleotide Sequencing , Humans , High-Throughput Nucleotide Sequencing/methods , HLA-B Antigens/genetics , Exons , Histocompatibility Testing/methods , Base Sequence , Polymorphism, Single Nucleotide , Sequence Alignment , Sequence Analysis, DNA/methodsABSTRACT
HLA-A*01:01:01:112 differs from the HLA-A*01:01:01:01 allele by one nucleotide substitution in the 5'UTR.
Subject(s)
Bone Marrow , HLA-A Antigens , Humans , Alleles , Greece , 5' Untranslated Regions , HLA-A Antigens/geneticsABSTRACT
HLA-C*04:01:01:174 differs from the HLA-C*04:01:01:06 allele by one nucleotide substitution in the intron 5.
Subject(s)
Genes, MHC Class I , HLA-C Antigens , Humans , HLA-C Antigens/genetics , Greece , Alleles , IntronsABSTRACT
Characterization of the novel HLA-DPB1*02:01:68 allele in a 27-year-old Greek hematopoietic stem cell transplant candidate.
Subject(s)
Hematopoietic Stem Cell Transplantation , Humans , Adult , Alleles , Greece , HLA-DP beta-Chains/geneticsABSTRACT
Characterization of the novel HLA-B*51:380 allele in a Greek individual using next-generation sequencing.
Subject(s)
HLA-B Antigens , High-Throughput Nucleotide Sequencing , Humans , Alleles , Greece , HLA-B Antigens/genetics , MutationABSTRACT
Characterization of the novel HLA-A*01:426 allele in a 21-year-old Greek male bone marrow donor.
Subject(s)
Bone Marrow Transplantation , Bone Marrow , Humans , Male , Young Adult , Adult , Alleles , Greece , Histocompatibility Testing , Tissue Donors , HLA-A Antigens/genetics , High-Throughput Nucleotide SequencingABSTRACT
The present cross-sectional study consists of a comprehensive analysis of epidemiological, laboratory, and clinical characteristics of COVID-19 patients in relation to their immunogenetic profiles. We studied 125 COVID-19 patients comprising different stages of disease severity; non-hospitalized (mild n = 69) and hospitalized (n = 56). Analysis of disease characteristics revealed no major differences between males and females of each group of patients while hospitalized patients were older and presented with comorbidities. A positive allele association was observed for HLA-DRB1*01 in total COVID-19 patients versus healthy controls. Subgrouping of COVID-19 patients in mild and hospitalized further identified a statistically significant increase in HLA-DRB1*01 in mild COVID-19 patients versus controls. The frequency of A*11, A*23, and DRB1*09 alleles was higher, while the frequency of C*12 was lower, in hospitalized patients versus healthy controls albeit with uncorrected statistical significance. The identification of specific allele associations may provide useful future markers for disease susceptibility in order to allow successful clinical management of COVID-19 patients.
ABSTRACT
The present study aimed to analyse and compare the distribution of MICA (rs1051792) and NKG2D/KLRK1 (rs1154831, rs1049174, rs2255336) polymorphisms in 61 Greek and 100 Polish patients with rheumatoid arthritis in relation to the presence of the HLA-DRB1 shared epitope and clinical parameters. Genotyping of selected polymorphism was performed using real-time PCR. HLA-DRB1 shared epitope alleles segregated differently in Greek and Polish patients but in both populations were detected in over 60% of cases. The rs1051792-A variant was more common among SE-positive Polish patients (p = 0.003) while the rs1049174-G allele was more frequently observed in Greeks than in Poles (p < 0.001). Moreover, among Greek patients, the rs1051792-GG homozygotes more frequently presented with anti-CCP antibodies and rheumatoid factor (RF), while carriers of the rs1049174-G variant and rs1154831-CC homozygotes were characterized by lower disease activity scores (p < 0.05 in all cases). These results imply that, in addition to the HLA-DRB1 SE alleles, MICA and NKG2D polymorphisms may also play a role in rheumatoid arthritis.
ABSTRACT
The present study proposes an integrated system based on the synergetic action of solar photocatalytic oxidation with surface flow constructed wetlands for the purification of wastewater contaminated with pesticides. Experiments were conducted at pilot scale using simulated wastewater containing the herbicide clopyralid. Three photocatalytic methods under solar light were investigated: the photo-Fenton and the ferrioxalate reagent as well as the combination of photo-Fenton with TiO2 P25, which all led to similar mineralization rates. The subsequent treatment in constructed wetlands resulted in further decrease of DOC and inorganic ions concentrations, especially of NO3-. Clopyralid was absent in the outlet of the wetlands, while the concentration of the detected intermediates was remarkably low. These findings are in good agreement with the results of phytotoxicity of the wastewater, after treatment with the ferrioxalate/wetlands process, which was significantly reduced. Thus, this integrated system based on solar photocatalysis and constructed wetlands has the potential to effectively detoxify wastewater containing pesticides, producing a purified effluent which could be exploited for reuse applications.