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Uncovering mechanisms of RT-LAMP colorimetric SARS-CoV-2 detection to improve assay reliability.
Suarez, Gianmarco D; Suarez, Domenick A; Kiu Tang, Yuki Yu; Zhang, Jing-Xuan; Li, Jun; Nagl, Stefan; Cheung, Peter Pak-Hang.
Anal Methods ; 14(4): 378-382, 2022 01 27.
Article in English | MEDLINE | ID: mdl-35006230

ABSTRACT

Improved diagnostics are needed to manage the ongoing COVID-19 pandemic. In this study, we enhanced the color changes and sensitivity of colorimetric SARS-CoV-2 RT-LAMP assays based on triarylmethane dyes. We determined a mechanism for the color changes and obtained sensitivities of 10 RNA copies per microliter.


Subject(s)
COVID-19 , SARS-CoV-2 , Colorimetry , Humans , Molecular Diagnostic Techniques , Nucleic Acid Amplification Techniques , Pandemics , RNA, Viral/genetics , Reproducibility of Results
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