The measurement of wound tensile strength and the effect of PRP on wound tensile force: an experimental investigation on rabbits.

Platelets in PRP are used for their functions in the initiation and regulation of the wound healing process and are used for the repair of injured tissues and the rejuvenation of healthy tissues. In this study, we evaluated the effect of a single dose platelet-rich plasma on skin wound healing and we demonstrated the effect of platelet-rich plasma on skin wound healing by measuring changes in the wound tensile strength.Material and methods: A total of 8 incisions, each 3 cm long, were made on the back skin on both sides of the vertebral column of 12 rabbits. After suturing their backs with staples, platelet rich plasma (PRP) was injected into the edges of the wounds on the left side and saline solution (saline) was injected into the edges of the wounds on the right side. The tensile force that causes wounds to rupture by applying tension was measured on the 7th, 14th, 21st, and 28th days with the help of a special home-designed device.Results: The mean PRP enrichment was 3.19 fold over peripheral blood. The saline to PRP tensile strength ratios on the 7th, 14th, 21st, and 28th days were calculated as 75.7%, 104.0%, 105.3% and 86.5%, respectively. Overall, the difference in the tensile strength for wounds that had received saline or PRP was in-significant.Conclusion: The application of PRP increases the tensile strength of the wound in the early period. It is possible to measure the tensile strength precisely in in vivo studies with economical home-designed devices.

Platelet-Rich Plasma for Skin Graft Storage: An Experimental Study Using Rabbit Ears.

BACKGROUND: Storage of surplus grafts for later use is one of the standard procedures used in plastic surgery. For the delayed use of skin grafts, various methods and media have been investigated for short-term storage. This study aimed to investigate the effect of platelet-rich plasma (PRP) skin graft storage on the survival of skin grafts obtained from rabbit ears. MATERIALS AND METHODS: Twelve rabbits were used in this study. A total of 12 skin grafts measuring 1 × 1 cm were obtained from the inner surfaces of the rabbits' ears. The grafts were stored at +4°C in saline, Hartmann's, and PRP media. On days 3, 7, 10, and 14, the grafts were implanted into the ears in areas measuring 1 × 1 cm where the skin, cartilage, and perichondria were excised. After the implantation of the grafts, the survival rates were evaluated by measuring the graft areas on day 0, day 10, and day 30. RESULTS: The graft survival rate decreased as the storage period increased in all 3 of the media. The decrease in survival rate was higher in the grafts that were stored in the Hartmann's media in comparison with the saline and PRP media, and the difference was statistically significant. The decrease in graft survival was similar between the storage in saline and PRP media; however, the differences were statistically insignificant. CONCLUSIONS: Although in vitro criteria are important for evaluating graft survival, in vivo studies showing the graft take rate in the recipient area are required. When the in vivo criteria are evaluated, the use of PRP is not superior to the use of saline for graft storage. However, additional studies are required to evaluate the effects of PRP media on graft quality.

Skin graft storage in platelet rich plasma (PRP).

Storage of skin grafts for later use is one of the standard applications in surgery. It is the most preferred method to maintain at +4°C in refrigeration after wrapping the surplus grafts into sterile gauze pad moistened with saline. Although there are many studies on the storage of skin grafts, less is known about storing skin grafts with PRP. Twenty-five pieces of 1 × 1 cm2 partial thickness skin graft were harvested from 12 patients during the reduction mammoplasty operation. Twenty-four grafts were divided into 4 groups, and each group consisted of 6 grafts, 1 graft was analyzed as Day 0. Grafts in Group 1, 2, and 3 were wrapped by sterile gauze pad moistened by either saline (Group 1) or Hartman (Group 2) or PRP (Group 3). Grafts were analyzed macroscopically and microscopically. There were no significant differences between media for the first 10 days. Decrease in viability was less in saline and PRP wrapped grafts at 20 day, viability decreased significantly in all environments after 20 days. Although there was no significant difference in saline or PRP storage, it was observed macroscopically that the grafts stored in the PRP appeared better.

Effects of device variables to radiofrequency (RF) applications.

RF devices have frequency, power and duration setting options, it is important to make sure that the device meets the targeted values at the head output. This study was made to evaluate the RF device output value accuracy and the effects of different frequencies on the tissue heat levels. RF was applied to invitro tissues obtained from surgical operations, and invivo tissues during operations. Heat differences and depth were measured by laser/IR thermometer and thermal infrared camera. First, the output frequency and power values provided by the device were approved. Then, three three heads (monopolar, bipolar and tripolar) with three different frequencies (1,7, 20 MHz) were used. Depth of heat increase was evaluated in millimeters. The results showed that temperature increase varied between 10°C and 30°C at different depths using different frequencies. Heating of the skin with a radiofrequency device in a therapeutic dose is possible if the appropriate frequency and adequate power values are applied. Because the therapeutic temperature is close to the complication limit, the practitioner should be an expert using the device, well-knowledgeable about the regional skin structure and thickness, as well as be able to properly adjust the application doses in order to get therapeutic results.