ABSTRACT
The enhanced generation of singlet oxygen (1O2) during oxidation of farmorubicin in the Co(II) + H2O2 system was studied using chemiluminescent, fluorescent and spectrophotometic techniques. The influence of 1O2-quenchers, catalase, superoxide anion radical (O2*-) and hydroxyl radical (HO*) scavengers on the light emission was studied. The spectrophotometric determination of 1O2 was based on bleaching of p-nitrosodimethylaniline caused by an intermediate product of the reaction of 1O2 with imidazole, and was followed by monitoring the decrease in optical density at 440 nm.
Subject(s)
Antibiotics, Antineoplastic/chemistry , Epirubicin/chemistry , Oxygen/chemistry , Fluorescence , Free Radicals/chemistry , Luminescent Measurements , Optics and Photonics , Oxidation-Reduction , SpectrophotometryABSTRACT
The effect of 4-flavonil-1,4-dihydropyridine derivatives on a chemical system involving a superoxide radical anion was tested using the chemiluminescence and spectrophotometry methods. All tested compounds enhanced the light emission from the system. The obtained results indicated that the tested derivatives may catalyze the conversion of superoxide radicals, thus showing superoxide dismutase activity.
Subject(s)
Antioxidants/chemistry , Antioxidants/pharmacology , Dihydropyridines/chemistry , Dihydropyridines/pharmacology , Flavonoids/chemistry , Flavonoids/pharmacology , In Vitro Techniques , Luminescent Measurements , Spectrophotometry , Superoxides/chemistryABSTRACT
The effects of thymol (TOH), thymoquinone (TQ) and dithymoquinone (TQ2) on the reactions generating reactive oxygen species (ROS) such as superoxide anion radical (O2*-), hydroxyl radical (HO*) and singlet oxygen (1O2) were tested using the chemiluminescence (CL) and spectrophotometry methods. All tested compounds acted as scavengers of various ROS. The rate constant of 1O2-dimols quenching by thymol was calculated.
