ABSTRACT
Oxygen deficiency during critical illness is known to cause profound changes in cellular metabolism with subsequent organ dysfunction. Clinical treatment in these patients is focussed on rapid reoxygenation to avoid a prolonged impaired synthesis of cellular high-energy phosphates (ATP). The effect of this therapeutical intervention on the level of the cell, however, has not yet been objectivized. The aim of the present experimental study was to biochemically monitor different tissues during hypoxia and reoxygenation using in vivo microdialysis. Eighteen adult male CD-rats (412-469 g; Ivanovas, Kisslegg, Germany) were normoventilated under general anaesthesia (FiO2 = 0.21). Ten were then subjected to a period of hypoxia (FiO2 = 0.1, 40 min) and reoxygenated with FiO2 = 0.21, while eight control animals were continuously ventilated with FiO2 = 0.21. In addition to invasive haemodynamic monitoring, biochemical tissue monitoring was performed using CMA 20 microdialysis probes, which were inserted into the muscle (m), subcutaneous space (s), liver (l) and peritoneal cave (p) with semicontinuous analyses of lactate and pyruvate at intervals of 15 minutes. Hypoxia induced a significant decrease in mean arterial pressure compared to the control group (p < 0.05). At the same time significant increases in blood lactate (12.3 + 4.1 mmol/l (hypoxia) vs. 1.5 +/- 0.3 mmol/l (control); p < 0.05) and in negative base excess (17.3 + 7 mmol/l (hypoxia) vs. 2.6 + 1.8 mmol/l (control), p < 0.05) occurred. Compared to unchanged levels in the control animals, the interstitital lacate/pyruvate ratio in the investigation group rose to significantly higher values (455 + 199% of baseline (m), 468 + 148% (p), 770 + 218% (l) and 855 + 432% (s) (p < 0.05). An immediate return to the baseline values after the start of reoxygenation was noted in the L/P ratio during the observation period. Using microdialysis, it was possible to objectify the effect of oxygen deficiency and restoration on tissue metabolism. Regarding clinical and preclinical practice, microdialysis monitoring should be performed to include biochemical cellular effects as an additional target for therapeutical interventions.
