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1.
Mar Drugs ; 11(12): 4834-57, 2013 Dec 02.
Article in English | MEDLINE | ID: mdl-24317473

ABSTRACT

New isolates of Streptomyces champavatii were isolated from marine sediments of the Gotland Deep (Baltic Sea), from the Urania Basin (Eastern Mediterranean), and from the Kiel Bight (Baltic Sea). The isolates produced several oligopeptidic secondary metabolites, including the new octapeptide champacyclin (1a) present in all three strains. Herein, we report on the isolation, structure elucidation and determination of the absolute stereochemistry of this isoleucine/leucine (Ile/Leu = Xle) rich cyclic octapeptide champacyclin (1a). As 2D nuclear magnetic resonance (NMR) spectroscopy could not fully resolve the structure of (1a), additional information on sequence and configuration of stereocenters were obtained by a combination of multi stage mass spectrometry (MSn) studies, amino acid analysis, partial hydrolysis and subsequent enantiomer analytics with gas chromatography positive chmical ionization/electron impact mass spectrometry (GC-PCI/EI-MS) supported by comparison to reference dipeptides. Proof of the head-to-tail cyclization of (1a) was accomplished by solid phase peptide synthesis (SPPS) compared to an alternatively side chain cyclized derivative (2). Champacyclin (1a) is likely synthesized by a non-ribosomal peptide synthetase (NRPS), because of its high content of (D)-amino acids. The compound (1a) showed antimicrobial activity against the phytopathogen Erwinia amylovora causing the fire blight disease of certain plants.


Subject(s)
Geologic Sediments/chemistry , Peptides, Cyclic/chemistry , Streptomyces/chemistry , Amino Acid Sequence , Amino Acids/chemistry , Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Erwinia amylovora/drug effects , Geologic Sediments/microbiology , Oceans and Seas , Peptides, Cyclic/pharmacology , Phylogeny , Streptomyces/metabolism
2.
J Bacteriol ; 193(14): 3691-2, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21571991

ABSTRACT

Streptomyces, a branch of aerobic Gram-positive bacteria, represents the largest genus of actinobacteria. The streptomycetes are characterized by a complex secondary metabolism and produce over two-thirds of the clinically used natural antibiotics today. Here we report the draft genome sequence of a Streptomyces strain, PP-C42, isolated from the marine environment. A subset of unique genes and gene clusters for diverse secondary metabolites as well as antimicrobial peptides could be identified from the genome, showing great promise as a source for novel bioactive compounds.


Subject(s)
Genome, Bacterial , Seawater/microbiology , Streptomyces/genetics , Streptomyces/isolation & purification , Base Sequence , China , Molecular Sequence Data , Sequence Analysis, DNA , Streptomyces/classification
3.
J Bacteriol ; 193(5): 1276-7, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21183663

ABSTRACT

Bacillus subtilis is an aerobic spore-forming Gram-positive bacterium that is a model organism and of great industrial significance as the source of diverse novel functional molecules. Here we present, to our knowledge, the first genome sequence of Bacillus subtilis strain gtP20b isolated from the marine environment. A subset of candidate genes and gene clusters were identified, which are potentially involved in production of diverse functional molecules, like novel ribosomal and nonribosomal antimicrobial peptides. The genome sequence described in this paper is due to its high strain specificity of great importance for basic as well as applied researches on marine organisms.


Subject(s)
Bacillus subtilis/classification , Bacillus subtilis/genetics , Genome, Bacterial , Indian Ocean , Molecular Sequence Data , Water Microbiology
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