ABSTRACT
The Elecsys Troponin T third generation assay uses recombinant human cardiac troponin T as standard material. The new assay has a linear calibration curve. Thus, linearity problems observed with the second generation assay have been eliminated. The assay has high precision, especially at the low end of measuring range (inter-assay CV < 10% at 0.1 microgram/L). The new standardization does not change the cut-off value of 0.1 microgram/L. The use of recombinant human cardiac troponin T as standard material enables a reproducible and reliable standardization of troponin T assays.
Subject(s)
Chemistry, Clinical/standards , Coronary Disease/diagnosis , Troponin T , Biomarkers , Calibration , Coronary Disease/blood , Humans , Linear Models , Recombinant Proteins , Reference Values , Reproducibility of Results , Troponin T/analysis , Troponin T/bloodABSTRACT
Concanavalin A-stimulated murine spleen cells and antigen-stimulated B lymphocytes of normal mice express an antigen that reacts with goat antiserum against glycoprotein (gp) 70. Structural analysis of this antigen characterizes it as endogenous viral gp70 that is most likely of xenotropic origin. Activated nonspecific T suppressor cells and cytotoxic T lymphocytes express endogenous viral gp70, whereas nonactivated mouse T or B lymphocytes do not. The presence of endogenous retroviral gp70 is thus a novel marker for activated mouse lymphocytes in general.
