Langerhans cells shape postnatal oral homeostasis in a mechanical-force-dependent but microbiota and IL17-independent manner.

The postnatal interaction between microbiota and the immune system establishes lifelong homeostasis at mucosal epithelial barriers, however, the barrier-specific physiological activities that drive the equilibrium are hardly known. During weaning, the oral epithelium, which is monitored by Langerhans cells (LC), is challenged by the development of a microbial plaque and the initiation of masticatory forces capable of damaging the epithelium. Here we show that microbial colonization following birth facilitates the differentiation of oral LCs, setting the stage for the weaning period, in which adaptive immunity develops. Despite the presence of the challenging microbial plaque, LCs mainly respond to masticatory mechanical forces, inducing adaptive immunity, to maintain epithelial integrity that is also associated with naturally occurring alveolar bone loss. Mechanistically, masticatory forces induce the migration of LCs to the lymph nodes, and in return, LCs support the development of immunity to maintain epithelial integrity in a microbiota-independent manner. Unlike in adult life, this bone loss is IL-17-independent, suggesting that the establishment of oral mucosal homeostasis after birth and its maintenance in adult life involve distinct mechanisms.

Number of consecutive procedures after endodontic treatment to extraction: A 28-year retrospective study.

STATEMENT OF PROBLEM: After endodontic treatment, teeth may require additional treatment. Data regarding the number of treatments up to extraction after endodontic treatment are lacking. PURPOSE: The purpose of this retrospective study was to evaluate the number of consecutive restorative procedures performed on a specific tooth starting from endodontic treatment up to extraction. A comparison was made between crowned and uncrowned teeth. MATERIAL AND METHODS: A retrospective study was conducted using 28 years of data from a private clinic. The total number of patients was 18 082 and included 88 388 treated teeth. The data were collected for permanent teeth that received at least 2 consecutive retreatments. The data included tooth number, procedure type, date of procedure, total number of procedures conducted during the study period, extraction date, time from endodontic treatment to extraction, and whether the tooth had been crowned or not. Endodontically treated teeth were divided into 2 groups: extracted and nonextracted. In each group, a comparison was made between crowned and uncrowned teeth and between anterior and posterior teeth by using the Student t test (α=.05). RESULTS: In the non extracted group, teeth that were crowned required significantly (P<.05) fewer restorative treatments (mean ±standard deviation 2.9 ±2.1) than uncrowned teeth (5.01 ±2.98). For extracted teeth, the mean time from endodontic treatment until extraction was 10.39 years. Crowned teeth were extracted after a mean of 11.06 years and 3.98 treatments, while uncrowned teeth were extracted after a mean of 9.96 years and 7.22 treatments (P<.05). CONCLUSIONS: Endodontically treated teeth that were crowned required significantly fewer subsequent restorative procedures than uncrowned teeth and exhibited higher survival rates up to extraction.