[Interrelation between the content of thiamine phosphates and pyruvate dehydrogenase activity in the liver of rats with intensified lipogenesis]. / Issledovanie vzaimosviazi mezhdu soderzhaniem tiaminfosfatov i piruvatdegidrogenaznoi aktivnost'iu v pecheni krys s intensifitsirovannym lipogenezom.

Injection of thiamine (250 micrograms per 100 g of mass daily) to rats with alimentary intensification of lipogenesis decreases the pyruvate dehydrogenase activity in the liver, which is accompanied by an increase in the concentration of pyruvate and drop in the level of free fatty acids. Under these conditions the content of total thiamine is 1.5 times as high. The level of thiamine pyrophosphate and thiamine triphosphate rises significantly. When all parameters were determined in dynamics after a single thiamine injection, an inverse dependence is observed between the content of thiamine phosphoric esters, thiamine triphosphate in particular, and pyruvate dehydrogenase activity.

[Preparative isolation of thiamine triphosphate by means of cation-exchange resins]. / Preparativnoe poluchenie tiamintrifosfata s pomoshch'iu kationobmennykh smol.

KY-2 and KY-2-8 cation-exchange resins are described as employed for preparative isolation of thiamine triphosphate. When it is eluted from the mentioned resins by distilled water, the preparation needs no additional purification from low-molecular admixtures. The preparation purity was controlled by paper electrophoresis in 0.025 M citric-acid buffer (pH 3.8-4.0).