Higher number of steps is related to lower endogenous progesterone but not estradiol levels in women.

OBJECTIVES: Sex steroid hormones are important not only for reproduction but also for many aspects of women's health, including the risk of breast cancer. Physical activity has been shown to influence sex hormone levels in women. This study aimed to investigate a relationship between the average daily number of steps and the sex hormone (estradiol and progesterone) levels in premenopausal women. MATERIALS AND METHODS: Data were collected from 85 healthy, urban women of reproductive age who performed at least 180 minutes/week of moderate physical activity for two complete menstrual cycles. Physical activity was measured using wrist bands. Estradiol and progesterone concentrations were measured in daily-collected saliva samples in the second menstrual cycle. RESULTS: There was a significant negative association between the average number of steps taken daily and salivary progesterone levels after adjusting for potential confounding factors (age, BMI). Women who took more than 10,000 steps a day had significantly lower progesterone levels compared to women who took less than 10,000 steps. The association between physical activity and estradiol levels was statistically insignificant. DISCUSSION: Our results indicate that taking at least 10,000 steps a day reduces progesterone levels, but this intensity of physical activity may not be high enough to affect estradiol levels. Daily step tracking is a valuable element of health promotion, but currently recommended levels of physical activity may not be high enough for healthy premenopausal women to significantly reduce both sex hormone levels and thus their risk of postmenopausal breast cancer.

Social support during pregnancy and the risk of postpartum depression in Polish women: A prospective study.

Social support has been proposed as an important determinant of women's physical and emotional well-being during pregnancy and after childbirth. Our study aimed to examine the association between the risk of postpartum depression (PPD) and perceived social support during pregnancy. A web-based prospective study survey was conducted among Polish women. The level of social support was measured with the Berlin Social Support Scales during pregnancy. Four weeks after the birth the risk of PPD was assessed using the Edinburgh Postpartum Depression Scale. Data from 932 mothers aged 19-43 (mean 30.95; SD 3.83) were analyzed using multinomial logistic regression. Higher perceived available support (emotional and instrumental), currently received support (emotional, instrumental and informational), satisfaction with the support, and sum of score were all associated with lower risk of PPD, after controlling for selected covariates (woman's age, socioeconomic status, parity status, place of residency, education, child's Apgar score, type of delivery, complications during birth, kin assisting the labor, breastfeeding). Our results suggest that the more social support the pregnant woman receives, the lower is her risk of PPD. Since humans evolved as cooperative breeders, they are inherently reliant on social support to raise children and such allomaternal help could improve maternal well-being.

Extreme pubic hair removal as a potential risk factor for recurrent urinary tract infections in women.

Urinary tract infections (UTIs) are the most common infections experienced by women. Previously, scalp and facial hair in men have been shown to inhibit the growth of pathogenic bacteria. Here we hypothesize that having hairy genitalia might protect women from UTI. This study investigated grooming habits and occurrence of UTIs in the past 12 months in 2409 women (aged 18-45). Women who reported removing all their pubic hair at least weekly were defined as extreme groomers (66.8%). We collected additional information on covariates including age, having a first UTI at or before age 15, spermicide use, having a new sex partner, and frequency of sexual intercourse during the past year. Extreme grooming was not associated with the risk of being diagnosed with UTI (OR = 1.17, 95% CI = 0.90-1.52), but was associated with a higher risk of recurrent UTIs, defined as three or more UTIs within 12 months (OR = 3.09, 95% CI = 1.35-7.06), after controlling for age, history of UTIs, and sexual practices. Other studies have found that hygienic purposes are the most common motivations for pubic hair removal. These results suggest that along with their pubes, women may be getting rid of important microbial niche and protection against recurrent UTIs.

Early-life adversities and later-life reproductive patterns in women with fully traced reproductive history.

One of the most crucial determinants of early-life development is the experience of childhood adversities. However, limited evidence is available for how these experiences shape later-life reproductive outcomes in women. Here we test the association between early-life adversities and reproductive parameters in women. Post-reproductive women (N = 105; mean age = 59.7; SD = 10.09) were recruited at the Mogielica Human Ecology Study Site in Poland within a traditional population with a low prevalence of birth control usage and fully traced reproductive histories. Reproductive parameters, as well as exposure to early-life abuse and neglect, were assessed using questionnaires. Childhood adversity was associated negatively with age at menarche (p = 0.009). Analyses on specific subtypes revealed that compared to women who did not experience any kind of early-life adversities in childhood, those who were exposed to emotional (p = 0.007) and physical (p = 0.023) neglect had an earlier age at menarche, those who experienced emotional abuse reported an earlier age at first birth (p = 0.035), and those who were exposed to physical abuse gave birth to fewer number of sons (p = 0.010). Our results suggest that women exposed to childhood adversities experience earlier physiological reproductive readiness and timing of the first birth, but their overall biological condition might be impaired as they bear fewer sons.

The age at first reproduction as a potential mediator between facial fluctuating asymmetry and reproductive success in women.

OBJECTIVES: The level of fluctuating asymmetry is suggested as a putative signal of developmental stability, thus according to this theoretical framework more symmetric individuals should be in better biological condition and have greater reproductive potential. Here we hypothesize that women with more symmetric faces have more successful reproduction. METHODS: Data were collected from 164 postmenopausal Polish women. Facial photographs were taken and the overall facial asymmetry (OFA) was calculated. The associations between the OFA and reproductive parameters were analyzed using multiple regression models. Furthermore, the mediation analysis was conducted to test for the indirect effects of the OFA on reproductive success. RESULTS: There was a statistically significant relationship between the OFA and the number of children born, which was mediated by the age at first reproduction (p = 0.03), however, the size of the effect was rather low. Women with more symmetric faces had an earlier age at first reproduction and, in consequence, a greater number of children. DISCUSSION: As fluctuating asymmetry is suggested to be established in utero, these findings shed light on the possible life-long importance of developmental conditions in shaping women's reproductive potential and performance.

Antenatal Classes in the Context of Prenatal Anxiety and Depression during the COVID-19 Pandemic.

Perinatal maternal anxiety and depression negatively affect intrauterine fetal development, birth outcome, breastfeeding initiation, duration, and milk composition. Antenatal classes potentially reduce the anxiety of pregnant women and may thus contribute to healthy infant development. The study investigates the relationship between participation in online or in-person antenatal classes and levels of anxiety and depression in Polish women during the COVID-19 pandemic. The study group included 1774 adult, non-smoking pregnant women. We compared the state anxiety (STAI-State) and depression levels (EPDS) in women who (i) attended antenatal classes in-person, (ii) attended online classes, and (iii) did not attend any of them. The statistical analyses included a GLM model and trend analysis, while controlling for maternal trait anxiety, age, pregnancy complications, trimester of pregnancy, previous pregnancies, and COVID-19 infections. We observed statistically significant differences in the level of anxiety (and depression). Women who did attend antenatal classes in person had the lowest levels of anxiety and depression. Considering the importance of maternal mental well-being on fetal development, birth outcome, and breastfeeding, in-person participation in antenatal classes should be recommended to pregnant women.

Inflammaging: Blame the sons. Relationships between the number of sons and the level of inflammatory mediators among post-reproductive women.

OBJECTIVES: Reproduction is costly, but sons and daughters differently influence maternal physiology, also in older age. In particular, having sons may negatively influence maternal health and may be associated with a shorter life span of mothers. Sons may also contribute to increased inflammaging, a chronic sub-clinical systemic inflammatory state characterized by elevated levels of serum inflammatory mediators. The aim of this study was to examine the impact of the total number of children, and the number of daughters and sons separately on concentrations of C-reactive protein (CRP), and proinflammatory cytokines such as interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α). MATERIALS AND METHODS: The participants were 378 women aged 45-92 who had 3.9 (SD 2.12, median = 4, range = 0-13) children, including 2.1 (SD 1.46, median = 2, range = 0-8) sons and 1.8 (SD 1.44, median = 2, range = 0-7) daughters on average. RESULTS: We found a positive relationship between the overall number of children and IL-6 levels. CRP and IL-6 concentrations were positively associated with the number of sons but not with the number of daughters. Each son increased maternal CRP level by 11%, and IL-6 level by 6%. Neither the total number of children nor the number of daughters or sons were related to the TNF-α concentration. DISCUSSION: Aging-associated inflammation in post-reproductive mothers with a higher number of sons supports the hypothesis of trade-offs between reproduction and health. Furthermore, these results provide new evidence contributing to the idea that having sons may have more detrimental effects on the maternal organism than having daughters.

Season of birth and biomarkers of early-life environment.

OBJECTIVE: Early-life conditions play an important role in human development, affecting health status and survival. Conditions in utero partly depend on the external environment and thus vary in relation to the season of birth. The aim of this study was to investigate if people born in different seasons of the year differ in values of biomarkers that reflect conditions during fetal development. METHODS: The study was conducted among Polish rural women recruited at the Mogielica Human Ecology Study Site. The participants were 234 women aged 45 to 92 (mean = 60.2; SD = 10.44). The indicators of early-life environment analyzed in the study were: Absolute Finger Ridge Count (AFRC), the difference between mean number of ridge counts in both thumbs and both little fingers (Md15), overall facial fluctuating asymmetry (OFA), central facial asymmetry (CFA), right and left hand 2D:4D. RESULTS: Values of biomarkers of fetal development did not vary among groups of women born in different seasons of the year. CONCLUSIONS: Lack of differences in values of biomarkers according to birth season may indicate that: (a) season of birth is not a good indicator of early-life conditions; (b) tested biomarkers do not reliably reflect the prenatal environment; (c) season of birth does not fully overlap with the sensitive periods of biomarker development and thus fails to capture differences in developmental conditions.

Digit ratio (2D:4D) is not related to cardiovascular diseases or their risk factors in menopausal women.

OBJECTIVES: Digit ratio (2D:4D) is widely used as a biomarker of prenatal hormonal environment linked to the growing number of adult health and disease-related characteristics. It has been suggested that 2D:4D is a good predictor of cardiovascular diseases (CVD) risk among men, but results in women are still inconclusive. Here we test the relationship between 2D:4D and the incidence of cardiovascular diseases (CVD), and their risk factors in Polish, rural women. METHODS: The participants were 410 women age 50 and older. Structured questionnaire was used to gather personal and medical data, including the history of CVD diagnosed by a medical doctor. Anthropometric measurements of body height, weight, and finger lengths were performed. Right-hand and left-hand 2D:4D, mean 2D:4D, Dl-r , and BMI were calculated afterward. For a subgroup of participants (n = 329) fasting blood sample was collected (in order to assess the lipid profile and glucose levels) and blood pressure was measured. Age, education level and BMI were included as potential covariates. RESULTS: No statistically significant association was observed between 2D:4D markers and the incidence of CVD (eg, heart attack, stroke) or CVD risk factors (dyslipidaemia, dysglycaemia or hypertension), when controlled for age, education and BMI. CONCLUSIONS: The results of our study add to the growing number of studies investigating the sex-difference of the association between 2D:4D and cardiac health. We conclude that from a public health perspective 2D:4D may not be a valuable biomarker of elevated risk of CVDs in women.

Biomarkers of fetal conditions: Finger ridge-counts, facial fluctuating asymmetry, and digit ratio (2D:4D)-are they correlated in women?

OBJECTIVES: Fetal environmental conditions are crucial for life-long health. Direct measurements of developmental conditions are limited in humans; thus, several biomarkers of those conditions have been proposed: that is, finger ridge-counts, level of facial fluctuating asymmetry (FA), and digit ratio (2D:4D). Since all of these biomarkers share a similar gestational time of formation, we hypothesize that their values are significantly correlated. MATERIALS AND METHODS: Data were collected at the Mogielica Human Ecology Study Site in southern Poland among 234 women. Finger ridge-counts, level of facial FA, and 2D:4D have been measured. The two-step analyses included Pearson's correlations of simple values of the biomarkers and correlations of composite variables calculated based on principal component analysis. RESULTS: We did not find any statistically significant correlations between finger ridge-counts, FA, and 2D:4D in women. Similarly, we did not observe any correlations between three composites created from the biomarkers. DISCUSSION: Our results indicate that there are no relationships between the biomarkers, suggested as proxies of the quality of prenatal conditions, in a single population. This is the first study analyzing three different markers simultaneously. The lack of correlations may indicate that the tested biomarkers reflect, in fact, different environmental conditions, occurring in separate "critical windows" of development, or that the biomarkers are not valid as proxies of developmental conditions.

Oxidative stress as a hidden cost of attractiveness in postmenopausal women.

Perceived facial attractiveness, a putative marker of high biological fitness, is costly to maintain throughout a lifetime and may cause higher oxidative stress (OS). We investigated the association between the facial features of 97 postmenopausal women and their levels of OS biomarkers 8-hydroxy-2'-deoxyguanosine (8-OHdG), superoxide dismutase (Cu-Zn SOD), and thiobarbituric acid reactive substances (TBARS). In study 1, 966 judges rated the composites (facial averages) of women with higher OS as more attractive, healthier, younger, and less symmetric. In study 2, Geometric Morphometric analysis did not reveal significant differences in facial morphology depending on OS levels. In study 3, measured facial averageness and symmetry were weakly negatively related to 8-OHdG levels. Maintaining higher perceived facial attractiveness may be costly due to increased oxidative damage in the postmenopausal period. These costs may remain hidden during the reproductive period of life due to the protective mechanisms of oxidative shielding and revealed only after menopause when shielding has ceased.

Declining ages at menarche in an agrarian rural region of Poland.

OBJECTIVE: Age at menarche in Poland has varied with political and socioeconomic changes. An increase in age at menarche corresponded to a period of economic crisis and food rationing between 1976 and 1989. Experiencing food shortages in utero or during childhood development can affect menarcheal timing, but this national effect may be buffered in local agrarian regions growing their own food. Here we examine patterns of age at menarche over time in the rural, agrarian Beskid Wyspowy region of southern Poland. METHODS: This study examined menarcheal timing using data collected from Polish women (n = 1326) recruited at the Mogielica Human Ecology Study Site between 2003 and 2018. Simple linear regressions were used to assess changing ages at menarche over time. Comparisons between ages at menarche for women born before and after the fall of communism in 1989 were assessed via one-way analysis of variance. RESULTS: Age at menarche has declined over time in the Beskid Wyspowy region of southern Poland from 1920 to 2000 (R2 = .08, P < .0001). There was not a statistically significant increase or decrease in age at menarche for women born and growing up during the period of food rationing. CONCLUSIONS: The declining age at menarche is likely reflective of a transitioning environment, suggesting that major socioeconomic changes affect life history traits like pubertal timing. Living in agricultural regions may have helped buffer the increasing ages at menarche seen in other areas of Poland during times of food rationing.

Sons May Be Bad for Maternal Health at Older Age: New Evidence for Costs of Reproduction in Humans.

High investment in reproductive processes at the expense of other physiological functions may result in poor maternal health. Health status at older age can be reliably assessed by self-rated health, which is a strong predictor of mortality. We investigated trade-offs between reproduction and health by analysing relationships between self-rated health and the total number of children ever born by a woman, and the number of her sons and daughters among 559 post-reproductive women aged 45-92 from Poland. The number of sons, but not the number of daughters or the total number of children, was significantly associated with self-rated health, adjusted to potential confounders (age, education, marital status, current smoking, and body mass index). Each son increased the odds of reporting poor health by 11% (odds ratio = 0.89; 95% confidence interval = 0.81-0.99). Sons are more energetically and immunologically demanding for the maternal organism which may explain why having sons has more negative influence on maternal health in older age than having daughters. In order to understand trade-offs between reproduction and maternal health not only the total number of children but also sex of the children should be taken into account.

Digit ratio (2D:4D) and circulating testosterone, oestradiol, and progesterone levels across the menstrual cycle.

BACKGROUND: Digit ratio (2D:4D) is used by researchers as an indicator of prenatal sex hormone exposure. Two previous studies have examined associations between 2D:4D and circulating sex steroid concentrations across the menstrual cycle in adult females. One reported that digit ratio correlated positively with oestradiol levels, whereas the other found no such effect; neither observed significant associations with progesterone. AIMS: To examine associations between 2D:4D, as well as asymmetry (i.e. right minus left 2D:4D), and circulating sex steroids across the menstrual cycle. STUDY DESIGN: Correlational. SUBJECTS: 32 naturally cycling adult females from rural southern Poland. OUTCOME MEASURES: Salivary oestradiol, progesterone, testosterone, and testosterone to oestradiol ratio (T:O) measured during the follicular, peri-ovulatory, and luteal phases. Average levels across the cycle were also examined. RESULTS AND CONCLUSIONS: Asymmetry in digit ratio correlated positively with oestradiol at each phase, as well as with average levels across the cycle. Each association, other than that relating to average levels, remained statistically significant after a range of covariates had been controlled for. No other significant correlations were observed between digit ratio variables and circulating hormone levels. Our results might suggest that low exposure to androgens and/or high exposure to oestrogens during gestation is a predictor of high oestradiol levels in naturally cycling females of reproductive age. However, considering that it was asymmetry in digit ratio, and not either right or left 2D:4D, that was a significant predictor, it is also possible that these effects reflect more general associations between bilateral asymmetry and circulating oestradiol levels.

Age difference between parents influences parity and number of sons.

OBJECTIVES: Among couples, women usually prefer slightly older men, and men tend to choose much younger partners. Age difference between partners has been shown to influence their parity; however, results of previous studies are inconsistent. This study analyzed relationships between husband and wife age difference and their total number of children, and number of daughters and sons in a contemporary, rural Polish population. METHODS: Demographic and reproductive data were collected from 384 postmenopausal women from rural Poland who were married only once. Regression models were used to evaluate the impact of the age gap between partners on total number of children and on number of daughters and sons. Women's age, age at marriage (as an indicator of reproductive value), and years of education were used in analyzes as potential confounders. RESULTS: There was an inverted U-shape association between parental age difference and number of children and also the number of sons. The highest number of children and sons was observed when men were approximately 6.5 years older than their wives. There was no significant relationship between parental age difference and number of daughters. CONCLUSIONS: Age difference between partners is important for reproductive success (with younger wives having higher reproductive potential) and is also related to number of sons. Older husbands might provide more resources for the family, thus facilitating production of well-nourished male offspring. Future research should evaluate not only number of children but also their biological condition, health, and lifetime achievements in relation to the age difference between their parents.

Costs of reproduction are reflected in women's faces: Post-menopausal women with fewer children are perceived as more attractive, healthier and younger than women with more children.

OBJECTIVES: The costs associated with reproduction (i.e., gestation, lactation, childcare) have long-term negative consequences by elevating risk of disease and reducing lifespan. We tested the hypotheses that high parity, and thus high reproductive costs bear by women, is perceived by other people when they evaluate facial appearance of health, attractiveness and age of mothers. MATERIALS AND METHODS: Using computer software we created average facial images based on real photographs of post-menopausal women with varying number of children; 3 parity categories were created (1-2, 4-5, and 7-9 children). Study participants (N = 571) were asked to choose the face they perceived as more attractive, younger and healthier via two-alternative forced choice questions asked in three randomized blocks. RESULTS: Women who had given birth to fewer children were judged both by men and women as more attractive, younger and healthier than women with more children. In each category the lowest scores were received by women from highest parity category (7-9 children). DISCUSSION: Mechanisms behind the observed variation in facial appearance are not known but higher levels of oxidative stress among women with high parity may explain their faster aging and lower attractiveness in older age. These results suggest that costs of reproduction might affect women's physical appearance.

Value of digit ratio 2D:4D, a biomarker of prenatal hormone exposure, is stable across the menstrual cycle.

Digit ratio (2D:4D) is used as a marker of prenatal hormone exposure and, consequently, as a predictor of many characteristics throughout a woman's lifespan. A previous study has suggested that values of 2D:4D vary across menstrual cycles and further questioned the reliability of a single measurement of 2D:4D among cycling women, while another study failed to confirm these results. However, these studies estimated the timing of cycle phases based on a date of menstruation reported by participants and also had small sample sizes. For our study, we evaluated potential changes in 2D:4D values across a menstrual cycle in a group of women among whom the phases of the menstrual cycle were determined by hormonal (luteinizing hormone based) ovulation tests. We studied 32 naturally cycling women aged 22-37 from rural Poland. Lengths of second and fourth digits were measured based on scans of both hands taken three times (i.e. in the follicular phase, peri-ovulatory phase and luteal phase of the cycle) for each participant. No differences in 2D:4D value across the menstrual cycle were detected either when right-hand, left-hand, and mean 2D:4D for both hands were analysed, nor when difference in the 2D:4D value between hands (Dleft-right) was evaluated. We documented that 2D:4D is independent of the phase of the menstrual cycle and these findings suggest that among naturally cycling women, a value of 2D:4D can be reliably obtained from measurements taken during any day of the menstrual cycle.

Women with more feminine digit ratio (2D:4D) have higher reproductive success.

OBJECTIVES: Prenatal development has a long-lasting influence on biological condition and health. Second-to-fourth digit ratio (2D:4D) is suggested as an indicator of sex hormone exposure during fetal development, and it is likely that women with a more feminine digit ratio were exposed to higher prenatal estrogen levels. Therefore, we tested if 2D:4D was related to a woman's reproductive characteristics. METHODS: We studied 319 women aged 46-92, who went through a natural menopause and whose husbands were alive at least until their menopause were studied. Women were recruited at the Mogielica Human Ecology Study Site located in rural Poland. RESULTS: Women with more feminine 2D:4D had a higher number of children (P = 0.002), gave birth to their last child at a later age (P = 0.02), and had a longer reproductive lifespan (P = 0.04) than women with more masculine 2D:4D. Age and number of years of education were included as potential confounders in the analyses. CONCLUSIONS: The results indicated that women with more feminine 2D:4D had higher reproductive success. While the exact mechanisms were not known, and the relationship between 2D:4D and adult sex hormone levels was questioned by previous studies, there might be other biological pathways explaining the observed results, that is, via behaviors that were indirectly related to fertility. Am J Phys Anthropol 160:549-553, 2016. © 2016 Wiley Periodicals, Inc.

Evidence for the Cost of Reproduction in Humans: High Lifetime Reproductive Effort Is Associated with Greater Oxidative Stress in Post-Menopausal Women.

Life history theory predicts trade-offs between reproductive effort and maternal survivorship in energy-restricted environments. However, empirical evidence for the positive association between maternal mortality and reproductive effort from energetically challenged human populations are mixed and physiological mechanisms that may underlie this association are poorly understood. We hypothesized that increases in aerobic metabolism during repeated periods of pregnancy and lactation result in increased oxidative stress that may contribute to somatic deterioration, vulnerability to illness, and accelerated aging. We therefore predicted that lifetime gravidity and parity would be related to levels of biomarkers of oxidative stress, as well as antioxidative defence enzymes in post-menopausal women. Our hypothesis was supported by positive linear associations between levels of 8-OHdG, a biomarker of DNA oxidative damage (ß = 0.21, p<0.05), levels of antioxidative defence enzyme Cu-Zn SOD (ß = 0.25, p<0.05), and number of lifetime pregnancies. Furthermore, independent of age and health status, post-menopausal women with higher gravidity and parity (> = 4 pregnancies per lifetime) had 20% higher levels of 8-OHdG and 60% higher levels of Cu-Zn SOD compared to women with lower gravidity and parity (<4 pregnancies per lifetime). Our results present the first evidence for oxidative stress as a possible cost of reproductive effort in humans.