[Vitamin E and its structural analogs in tuberculosis]. / Vitamin E i ego strukturnye analogi pri tuberkuleze.

Efficiency of tocopherol, its analog with a shortened side chain as well as their quinons for tuberculosis was determined. All the studied compounds inhibited peroxide-formation processes in the liver homogenate and mitochondria. The vitamin E amount in the blood is considerably decreased in case of tuberculosis. The studied analogs increased its content and possessed a weak tuberculostatic but expressed antiedemic and antiinflammatory action. Administration of vitamin E in combination with isoniazid to sick animals had a favourable effect on the processes of tissue respiration and oxidative phosphorylation. If taking a sum of characters, tocopherol acetate with a shortened side chain is the most efficient of all the analogs under study for the tuberculosis therapy.

[A new method of cultivating Mycobacterium tuberculosis]. / Novyi metod kul'tivirovaniia mikobakterii tuberkuleza.

The possibility is shown of microcultivation of Mycobacteria tuberculosis on semipermeable cellophane membranes covering solid nutrients. The new method of cultivation may be used for enhanced microbiological diagnosis of tuberculosis, for determination of drug resistance of the tuberculous pathogen as well as for assessment of the biological properties of Mycobacteria tuberculosis in live unstained culture.

[Bacteriostatic activity of blood in patients with pulmonary tuberculosis during polychemotherapy]. / Bakteriostaticheskaia aktivnost' krovi bol'nykh tuberkulezom legkikh pri polikhimioterapii.

Polychemotherapy comprising 4-6 antituberculosis drugs and prescribed either only daily, daily or given on an intermittent basis (every other day) or only on an intermittent basis was given for 4-8 months to 102 patients with destructive pulmonary tuberculosis (in those newly diagnosed, treated earlier and with a process recurrence). Four and, less frequently, three drugs were used per day. Bacteriostatic blood activity (BBA) was high in 90.2% of the patients, at the maximal concentrations, bacteriostasis was seen at 32-256-fold blood dilutions; in 76.4% of the patients BBA persisted at a high or moderate level during 24 hours (blood dilutions were within 1:256-1:8). The maximal and total BBA level was significantly higher in polychemotherapy than in a combination of 3 drugs. In polychemotherapy bacilli excretion was discontinued in 90% and caverns healed in 74.5% of the patients, while in the regimens comprising 3 drugs in the same contingent of patients, similar events occurred in 77% and 60% of the cases, respectively.

[Combined use of antitubercular drugs and metabolites of the tricarboxylic acid cycle in experimental tuberculosis]. / Sochetannoe primenenie protivotuberkuleznykh preparatov i metabolitov trikarbonovogo tsikla pri éksperimental'nom tuberkuleze.

The efficacy of a combined use of isoniazid together with polyzid prolonged tuberculostatic and tricarboxylic acid cycle metabolites was experimentally studied in guinea pigs. Mortality rates, pathomorphologic test data and the extent of tuberculous affection in the animals confirm a fairly high efficacy of prolonged polyzid in combination with succinate and malate as pathogenetic drugs.

[Investigation of pathogens in the foci of pulmonary and osteo-articular tuberculosis]. / Issledovanie vozbuditelia v ochagakh porazheniia pri legochnom i kostno-sustavnom tuberkuleza]

The tubercle bacilli detection rate was determined by direct bacterioscopy and the culture plate method immediately in the disease foci in 123 patients with pulmonary tuberculosis and 78 patients with tuberculosis of bones and joints. The culture plate method was shown to have significant advantages over bacterioscopy. However, in some cases with negative responses to the culture plate test, bacterioscopy was the only procedure that detected the pathogen in resected lung tissues. Parallel use of 2 different media in the culture plate test provided higher frequency of the pathogen isolation. Cultures isolated from the lung foci were showed drug resistance in 1/4 of the cases. Cultures from the bone foci were in all the cases but one sensitive to all the drugs used. By their biological properties, all the isolates belonged to human tubercle bacilli. The necessity of microbiological investigation of pathogenes in tuberculosis foci is grounded.

[Biological characteristics of rifampicin-resistant Mycobacterium tuberculosis]. / Biologicheskaia kharakteristika rezistentnykh k rifampitsinu mikobakterii tuberkuleza.

Eleven rifampicin resistant variants were obtained in vitro from rifampicin sensitive strains of M. tuberculosis. It was shown that the in-vitro development of the resistance to rifampicin was of the one-stage phenomenon type. The study on the morphological, tinctorial and cultural properties, catalase activity, cord factor and toxicity showed their identity in the initial rifampicin sensitive strains and the rifampicin resistant variants. The virulence of the rifampicin resistant variants for albino rats and rabbits did not significantly change either.