ABSTRACT
The positive correlation of micronuclei quantity appearance and catepsin D activation in mice cells after immunization by alive influenza vaccine was demonstrated. Activation of proteinase precedes chromosomal aberrations. Catepsin D activation is supposed to lead to proteolysis in achromatic apparatus which results in micronuclei formation.
Subject(s)
Cathepsin D/analysis , Influenza Vaccines/administration & dosage , Lung/ultrastructure , Micronuclei, Chromosome-Defective , Ammonium Chloride/pharmacology , Animals , Chromosome Aberrations , Enzyme Activation , Hydrocortisone/pharmacology , Male , MiceABSTRACT
The mechanisms of hydrocortisone and adrenalin action on the structure and function of the lysosomal-vacuolar cell apparatus were studied in experiments on liver sections of Wistar rats. The sections were incubated in Krebs-Ringer bicarbonate buffer, pH 7.4 (95% O2 and 5% CO2) at 37 degrees C for 2 h. Hydrocortisone (10(-5) M) and adrenalin (10(-4) M), added to an incubation medium, were shown to produce a labilizing effect on lysosomal membranes, increasing free activity of acid phosphatase and cathepsin D and osmotic sensitivity of lysosomes. alpha-adrenergic blocker dihydroergotamine (3.4 x 10(-5) M) blocked an increase in free activity of acid phosphatase as a result of adrenalin action but did not eliminate hydrocortisone labilizing action. beta-adrenergic blocker propranolol (3 x 10(-4) M) lowered free activity indices and osmotic sensitivity of lysosomes to control values both in the presence of adrenalin and hydrocortisone. The labilization of lysosomal membranes in liver sections was also observed after adding dibutyril-cAMP (10(-8) M) or monobutyril-cGMP (10(-13)-10(-9) M) into the incubation medium.
Subject(s)
Epinephrine/pharmacology , Hydrocortisone/pharmacology , Liver/drug effects , Lysosomes/drug effects , Animals , Dihydroergotamine/pharmacology , Drug Interactions , Female , In Vitro Techniques , Liver/enzymology , Lysosomes/enzymology , Nucleotides, Cyclic/pharmacology , Osmosis/drug effects , Propranolol/pharmacology , Rats , Rats, Inbred StrainsABSTRACT
Antitumour drugs prospidine and spirobromine were shown to suppress the activity of the mouse liver lysosome enzymes in vivo and in experiments in vitro. The revealed effect was underlied by a decrease of lysosomal membrane permeability for the corresponding substrates and enzymes rather than a reduction of the catalytic activity of acid hydrolases.
Subject(s)
Antineoplastic Agents/pharmacology , Liver/drug effects , Lysosomes/drug effects , Piperazines/pharmacology , Prospidium/pharmacology , Spiro Compounds/pharmacology , Animals , Antineoplastic Agents/pharmacokinetics , Cell Membrane Permeability/drug effects , Detergents/pharmacokinetics , Detergents/pharmacology , Hydrolases/metabolism , Liver/enzymology , Lysosomes/enzymology , Male , Mice , Octoxynol , Polyethylene Glycols/pharmacokinetics , Polyethylene Glycols/pharmacology , Prospidium/pharmacokinetics , Spiro Compounds/pharmacokineticsABSTRACT
Changes of cardiac, sceletal and hepatic lysosomes effected by three different variants of balanced isocaloric diets: 1--basic, 2--protein-lipid and 3--carbohydrate, with ratios of proteins, lipids and carbohydrates in mass 1.0:0.35:2.6; 1.0:0.48:0.67; and 1.0:1.3:10.9, respectively, were studied. Activities of enzymes participating in degradation of carbohydrates (beta-glucosidase, beta-N-acetyl-glucosaminidase) were more increased in the animals fed with the carbohydrate diet. Activation of proteolytic enzymes (cathepsin D) was observed in tissues of rats taking the protein-lipid food. As a rule physical exercise exerts lysosomal enzymes activation and their membrane labilization in rats maintained on the basic diet. Reversial reactions of lysosomes to physical exercise is observed in animals receiving the carbohydrate diet for along time that is expressed in increasing the total activity of lysosomal hydrolases and stabilizing the lysosomal membranes. On the contrary, maintaining the animals on the protein-lipid diet is followed by a more pronounced activation of the lysosomal apparatus of tissues as compared to the basic diet.
Subject(s)
Animal Nutritional Physiological Phenomena , Lysosomes/enzymology , Physical Exertion , Animals , Diet , Liver/enzymology , Male , Muscles/enzymology , Myocardium/enzymology , Rats , Rats, Inbred Strains , RestABSTRACT
Administration to rabbits of hydrocortisone (8 mg/kg) and adrenaline (0.3 ml of 0.1% solution) during mild dithizone diabetes simulated hormonal reconstructions of the body exposed to stress. Activation of the cellular lysosomal apparatus occurs in such hormonal situation. In the liver this in manifested by an increase in the acid phosphatase, cathepside D, and beta-glucosidase free activity and by reduction in the resistance of lysosomal membranes to the effect of osmotic shock. Increase in the total acid hydrolase activity was detected in the cardiac and skeletal muscles.
Subject(s)
Diabetes Mellitus, Experimental/enzymology , Epinephrine/pharmacology , Hydrocortisone/pharmacology , Lysosomes/drug effects , Animals , Diabetes Mellitus, Experimental/chemically induced , Dithizone/pharmacology , Liver/enzymology , Lysosomes/enzymology , Muscles/enzymology , Myocardium/enzymology , Rabbits , Stimulation, ChemicalABSTRACT
Using surviving rat liver slices, the influence of blood serum lipoproteins on cellular lysosomes was studied. Lipoproteins were isolated by the preparative isopicnic ultracentrifugation method. Apoproteins were separated by means of delipidation of suitable lipoprotein fractions. After preincubation of liver slices in media with high density lipoproteins (HDLP), low and very low density lipoproteins (LDDLP, VLDLP), apo-HDLP and apo-VLDLP, the rise of free acid hydrolase activities was seen. The stability of lysosomes in hypotonic solutions turned out to be low, and the tendency to lysosomal aggregation increased. Typical peribiliary localization of lysosomes in the cell remained unchanged. Following preincubation of liver slices with hydrocortisone, adrenalin and VLDLP or LDLP, the rise of free lysosomal enzyme activity, greater aggregation and lysosome polymorphism and their less osmotic resistance took place. After liver slice preincubation with hormones and HDLP, besides all of these changes, the lysosomal translocation to the nuclei was seen. When apoproteins, separately or together with hormones, were added, the same type of alteration of lysosomal membranes occurred. Our results suggest an importance of lipo (apo) proteins in the control of structure and functions of cellular lysosomal apparatus.