Use of interaction matrices to formalise the development of conceptual models of contaminant transport in the biosphere and the translation of those conceptual models into mathematical models.

In developing models of the biosphere for use in assessing the impacts on human health and the environment of releases of contaminants from disposal facilities for solid radioactive wastes or from contaminated legacy sites, there is a need to demonstrate that the models adopted are both comprehensive and appropriate to the assessment context. To achieve this end, it is useful to develop a structured approach to conceptual model development and it is here proposed that interaction matrices (IMs) provide a suitable framework. This process can provide a conceptual model expressed in terms of either a single IM or a nested set of IMs. The focus of the work described herein is the development of a transparent approach to translating such a set of IMs into a mathematical model, which is typically expressed as a set of ordinary differential equations complemented by algebraic expressions. Some remarks are also made on appropriate approaches to obtaining numerical solutions of these equations in circumstances where simplifications of the general equations can be justified. Overall, the intent is to provide background and guidance by providing a formal basis for the process in generalised terms.

Multistate outbreak of Salmonella Paratyphi B variant L(+) tartrate(+) and Salmonella Weltevreden infections linked to imported frozen raw tuna: USA, March-July 2015.

Foodborne non-typhoidal salmonellosis causes approximately 1 million illnesses annually in the USA. In April 2015, we investigated a multistate outbreak of 65 Salmonella Paratyphi B variant L(+) tartrate(+) infections associated with frozen raw tuna imported from Indonesia, which was consumed raw in sushi. Forty-six (92%) of 50 case-patients interviewed ate sushi during the week before illness onset, and 44 (98%) of 45 who specified ate sushi containing raw tuna. Two outbreak strains were isolated from the samples of frozen raw tuna. Traceback identified a single importer as a common source of tuna consumed by case-patients; this importer issued three voluntary recalls of tuna sourced from one Indonesian processor. Four Salmonella Weltevreden infections were also linked to this outbreak. Whole-genome sequencing was useful in establishing a link between Salmonella isolated from ill people and tuna. This outbreak highlights the continuing foodborne illness risk associated with raw seafood consumption, the importance of processing seafood in a manner that minimises contamination with pathogenic microorganisms and the continuing need to ensure imported foods are safe to eat. People at higher risk for foodborne illness should not consume undercooked animal products, such as raw seafood.

Climate change and landscape development in post-closure safety assessment of solid radioactive waste disposal: Results of an initiative of the IAEA.

The International Atomic Energy Agency has coordinated an international project addressing climate change and landscape development in post-closure safety assessments of solid radioactive waste disposal. The work has been supported by results of parallel on-going research that has been published in a variety of reports and peer reviewed journal articles. The project is due to be described in detail in a forthcoming IAEA report. Noting the multi-disciplinary nature of post-closure safety assessments, here, an overview of the work is given to provide researchers in the broader fields of radioecology and radiological safety assessment with a review of the work that has been undertaken. It is hoped that such dissemination will support and promote integrated understanding and coherent treatment of climate change and landscape development within an overall assessment process. The key activities undertaken in the project were: identification of the key processes that drive environmental change (mainly those associated with climate and climate change), and description of how a relevant future may develop on a global scale; development of a methodology for characterising environmental change that is valid on a global scale, showing how modelled global changes in climate can be downscaled to provide information that may be needed for characterising environmental change in site-specific assessments, and illustrating different aspects of the methodology in a number of case studies that show the evolution of site characteristics and the implications for the dose assessment models. Overall, the study has shown that quantitative climate and landscape modelling has now developed to the stage that it can be used to define an envelope of climate and landscape change scenarios at specific sites and under specific greenhouse-gas emissions assumptions that is suitable for use in quantitative post-closure performance assessments. These scenarios are not predictions of the future, but are projections based on a well-established understanding of the important processes involved and their impacts on different types of landscape. Such projections support the understanding of, and selection of, plausible ranges of scenarios for use in post-closure safety assessments.

A Multistate Investigation of Antibiotic-Resistant Salmonella enterica Serotype I 4,[5],12:i:- Infections as Part of an International Outbreak Associated with Frozen Feeder Rodents.

While most human Salmonella infections result from exposure to contaminated foods, an estimated 11% of all Salmonella infections are attributed to animal exposures, including both direct animal handling and indirect exposures such as cleaning cages and handling contaminated pet food. This report describes the epidemiologic, environmental and laboratory investigations conducted in the United States as part of the response to an international outbreak of tetracycline-resistant Salmonella enterica serotype I 4,[5],12:i:- infections with over 500 illnesses occurring from 2008 to 2010. This investigation found that illness due to the outbreak strain was significantly associated with exposure to pet reptiles and frozen feeder rodents used as food for pet reptiles. Salmonella isolates indistinguishable from the outbreak strain were isolated from a frozen feeder mice-fed reptile owned by a case patient, as well as from frozen feeder mice and environmental samples collected from a rodent producing facility (Company A). An international voluntary recall of all Company A produced frozen feeder animals sold between May 2009 and July 2010 occurred. Only 13% of cases in our investigation were aware of the association between Salmonella infection and mice or rats. Consumers, the pet industry, healthcare providers and veterinarians need to be aware of the potential health risk posed by feeder rodents, whether live or frozen. Frozen feeder rodent producers, suppliers and distributors should follow the animal food labelling requirements as described in 21 CFR §501.5, and all packages of frozen feeder rodents should include safe handling instructions. Persons should wash their hands thoroughly with soap and water after handling live or frozen feeder rodents, as well as reptiles or anything in the area where the animals live. Continued opportunities exist for public health officials, the pet industry, veterinarians and consumers to work together to prevent salmonellosis associated with pet food, pets and other animals.

Advanced spatio-temporal modelling in long-term radiological assessment models--radionuclides in the soil column.

Recent developments in the modelling of key radionuclides in long-timescale assessments of the safety of geological disposal of spent fuel and other radioactive wastes emphasise the influence of the redox conditions of the soil column. Models with higher spatial resolution than typically employed in standard modelling approaches have been shown to capture important features of experimental observations that are not otherwise manifested. Furthermore, models with monthly, rather than annually, averaged parameters and with dynamic transfers between soil and plant have been shown to lead to key differences compared with standard models employing soil-plant concentration ratios. This paper looks at the potential for the inclusion of a higher spatio-temporal resolution in models for long-timescale dose assessments and includes representations of measured plant-root distributions as well as the effects of bioturbation. Focusing here on the distribution and dynamics of radionuclides in the soil column, the effects of different spatial and temporal resolution are compared, together with an investigation of the way in which the hydrology of the soil column is represented. The approach has been successfully incorporated into a practical assessment-level model. Results indicate the potential importance of higher spatio-temporal resolution in modelling soil column dynamics, particularly of weakly sorbing radionuclides in long-timescale assessments featuring sudden transitions between ecosystem types.

Lung function in divers.

Correct lung function is indispensible to perform work underwater. Thus, spirometric tests of lung function remain an important element in the process of selecting candidates for professional diving. Studies conducted in the population of divers identified the phenomenon called 'large lungs', which is often associated with spirometric indices characteristic of obstructive impairment of lung function. This study investigated selected parameters of lung function in the population of divers and candidates for professional divers. Fifty two male subjects were examined as part of the selection process. Basic spirometric tests: forced expiratory volume in 1 s (FEV1; dm(3)), forced vital capacity (FVC; dm(3)), forced expiratory flow in the range 25-75 % of FVC (FEF25-75; dm(3) s(-1)), and FEV1/FVC (%) were compared with compared with the predicted reference values estimated by the European Coal and Steel Community. The results demonstrate differences in FVC and FEF25-75 in divers, which may correspond to functional hyperinflation. The effects of 'large lungs' observed in divers, if persisting for an extended period of time, may lead to lung ventilation impairment of the obstructive type.

Multistate outbreak of Listeria monocytogenes associated with Mexican-style cheese made from pasteurized milk among pregnant, Hispanic women.

Listeriosis is a severe infection caused by Listeria monocytogenes. Since 2004, the Centers for Disease Control and Prevention has requested that listeriosis patients be interviewed using a standardized Listeria Initiative (LI) questionnaire. In January 2009, states and the Centers for Disease Control and Prevention began investigating a multistate outbreak of listeriosis among pregnant, Hispanic women. We defined a case as an illness occurring between October 2008 and March 2009 with an L. monocytogenes isolate indistinguishable from the outbreak strain by pulsed-field gel electrophoresis. We conducted a multistate case-control study using controls that were selected from L. monocytogenes illnesses in non-outbreak-related pregnant, Hispanic women that were reported to the LI during 2004 to 2008. Eight cases in five states were identified. Seven of these were pregnant, Hispanic females aged 21 to 43 years, and one was a 3-year-old Hispanic girl, who was excluded from the study. Seven (100%) cases but only 26 (60%) of 43 controls had consumed Mexican-style cheese in the month before illness (odds ratio, 5.89; 95% confidence interval, 1.07 to ∞; P = 0.04). Cultures of asadero cheese made from pasteurized milk collected at a manufacturing facility during routine sampling by the Michigan Department of Agriculture on 23 February 2009 yielded the outbreak strain, leading to a recall of cheeses produced in the plant. Recalled product was traced to stores where at least three of the women had purchased cheese. This investigation highlights the usefulness of routine product sampling for identifying contaminated foods, of pulsed-field gel electrophoresis analysis to detect multistate outbreaks, and of the LI for providing timely exposure information for case-control analyses. Recalls of contaminated cheeses likely prevented additional illnesses.

Methods for conducting an economic opportunity survey in smallholder dairy farms.

A form was developed for on-farm use to collect data on dairy cow inventory, animals born, died, and sold, milk consumed and sold, and feedstuffs. Using these data, ten herd management indices are calculated in a spreadsheet. These data are gathered from multiple farms in an area and are summarized to develop herd target or benchmark values. Economic opportunities for achieving target performance at the individual farm level are calculated for five of the indices. This Economic Opportunity Survey has been used to develop regional performance benchmarks and to help individual farmers identify areas for improvement and opportunities for future intervention.