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1.
Quantitation of bovine immunoglobulins in culture fluids by use of sandwich radioimmunoassay with monoclonal antibodies.
Srikumaran, S; Kluever, E A; Onisk, D V; Hariharan, K.
Am J Vet Res ; 52(2): 243-6, 1991 Feb.
Article in English | MEDLINE | ID: mdl-2012336

ABSTRACT

Bovine immunoglobulin isotype-specific murine monoclonal antibodies were used in sandwich radioimmunoassays to detect and quantitate bovine IgG1, IgG2, IgM, and IgA in culture fluids. The concentrations of bovine immunoglobulins in unknown samples were extrapolated from standard curves generated with bovine monoclonal immunoglobulins. The lowest detection limits for the bovine immunoglobulin isotypes ranged from 65 to 270 ng/ml.


Subject(s)
Antibodies, Monoclonal , Immunoglobulins/analysis , Radioimmunoassay/veterinary , Animals , Cattle , Culture Media/chemistry , Immunoglobulin A/analysis , Immunoglobulin G/analysis , Immunoglobulin M/analysis , Radioimmunoassay/methods
2.
In vitro immunosuppression mediated by an extracellular polysaccharide from Capnocytophaga ochracea. Influence of macrophages.
Bolton, R W; Kluever, E A; Dyer, J K.
J Periodontal Res ; 20(3): 251-9, 1985 May.
Article in English | MEDLINE | ID: mdl-3160840

Subject(s)
Capnocytophaga/immunology , Cytophagaceae/immunology , Immunity, Cellular , Immunosuppression Therapy , Macrophages/immunology , Polysaccharides, Bacterial/immunology , Animals , Cells, Cultured , Lymphocyte Activation , Mice , Mice, Inbred C57BL , Receptors, Concanavalin A/immunology , Spleen/cytology , T-Lymphocytes/immunology
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