ABSTRACT
Morphological, functional signs of myocardium affection and tolerance to physical loading in animals with isoproterenol-induced affection of myocardium after transplantation of a nuclear-containing cells (NCC) of umbilical blood were analyzed in comparison with a natural course of the model. There was proved, that a transplantation of a NCC promotes acceleration of processes of regeneration and restoration of the damaged myocardium in experimental animals. There were estimated the changes in localization of the transplanted NCC in the zone of damage, and capacity of the peripheral blood NCC, while they were transplanted, using intravenous injection, to migrate into the damage zone. The experimental data obtained need further complex analysis and permit to wait a positive effect while performing clinical investigations in the patients, suffering chronic diseases of the heart.
Subject(s)
Cord Blood Stem Cell Transplantation , Fetal Blood/cytology , Myocardial Infarction/therapy , Myocardium/pathology , Myocytes, Cardiac/pathology , Animals , Cryopreservation , Female , Fetal Blood/physiology , Fetal Blood/transplantation , Humans , Isoproterenol , Mice , Myocardial Infarction/chemically induced , Myocardial Infarction/pathology , Regeneration/physiology , Transplantation, HeterologousABSTRACT
The detection of possibility differentiation towards adipogenic trend of the cells, which were obtained from adipose tissue, and their impact on the processes of adipose transplant resorption, in condition of its allogenic transplantation, have had constituted the objective of the investigation. Combined transplantation of adipose transplant and multipotent mesenchymal stem cells, obtained from allogenic adipose tissue, causes the destruction-inflammatory processes activation in the adipose transplant, as well as the adipocytes survival worsening in a meanwhile - a significant deficiency in the transplant mass.
Subject(s)
Adipocytes/pathology , Adipose Tissue/transplantation , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells/cytology , Multipotent Stem Cells/transplantation , Adipose Tissue/cytology , Animals , Cell Culture Techniques , Cell Differentiation , Cell Survival , Female , Humans , Mice , Mice, Transgenic , Multipotent Stem Cells/cytology , Transplantation, HomologousABSTRACT
There was conducted experimental investigation with objective to study up the influence of transplantation of a native allogenic multipotent mesenchymal stem cells on the processes of implantation and functioning of heterotopically transplanted autologous adipose tissue. In a cellular-tissue graft, in which native allogenic mesenchymal stem cells were applied as a cellular component, there was observed a worsening of the adipocytes survival indices as well as activation of destructive and inflammatory processes.
Subject(s)
Adipose Tissue/transplantation , Mesenchymal Stem Cell Transplantation/methods , Multipotent Stem Cells/transplantation , Adipocytes/pathology , Adipose Tissue/cytology , Animals , Cell Differentiation , Cell Survival , Female , Green Fluorescent Proteins/genetics , Mice , Mice, Transgenic , Microscopy, Fluorescence , Multipotent Stem Cells/cytology , Transplantation, Autologous , Transplantation, HomologousABSTRACT
Growth experiments of transplanted malignant tumors in the presence of water-soluble C60 fullerenes were performed on groups of mice. It was found that C60 fullerenes efficiently inhibit the growth of transplanted malignant tumors. This behavior can be explained through their high antioxidant activity and the blocking of the specific cell receptors (for example, endothelial growth factor). The findings demonstrate the possibility of using C60 fullerenes in anticancer therapy.
